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Peer-reviewedCC BY-NC-ND 4.0Translational Vision Science & Technology

In Vivo Wide-Field OCT Imaging Reveals and Quantifies a Hyporeflective Band beneath Bowman's Layer

  • Marco Ruggeri1,2
  • Yasufumi Tomioka3,4
  • Ngoc Lan Vy Truong1,2
  • Carolina Parra Rosas1,4
  • Elena Veronica Perozzo1,4
  • Francesco Pozzo Giuffrida1,4
  • Florence Cabot1,4
  • Jean-Marie Parel1,5
  • Chie Sotozono3
  • Shigeru Kinoshita6
  • Victor L. Perez4
  • Arthur Ho1,5,7
2 more authors
  • Mohamed Abou-Shousha4
  • Fabrice Manns1,2
7 affiliations
  1. 1Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA
  2. 2Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, FL, USA
  3. 3Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  4. 4Laboratory of Ocular Immunology & Transplantation, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA
  5. 5Brien Holden Vision Institute, Sydney, NSW, Australia
  6. 6Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  7. 7School of Optometry & Vision Science, University of New South Wales, Sydney, NSW, Australia
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Abstract

Purpose: To investigate the anterior corneal region immediately beneath Bowman's layer using wide-field self-referenced optical coherence tomography (OCT) and to identify and characterize a distinct hyporeflective band at the Bowman's layer–anterior stroma interface.

Methods: Seventeen eyes from 17 healthy subjects were analyzed with a custom wide-field spectral-domain OCT system in a self-referenced common-path configuration. Eighteen radial scans over a 9-mm corneal diameter were acquired and processed. A segmentation framework was developed to identify the boundaries of the epithelium, Bowman's layer, hyporeflective band, and total cornea. Thickness maps were generated and analyzed across four anatomical zones. Repeatability was assessed using repeated segmentations.

Results: A thin, continuous hyporeflective band (HB) immediately beneath Bowman's layer was consistently visualized across the corneal field in all 17 eyes analyzed. Mean HB thickness was approximately 8 µm, with slightly greater central than peripheral values. However, zonal differences remained within measurement repeatability. Thickness of the epithelium and Bowman's layer showed minimal spatial variation within measurement precision.

Conclusions: Wide-field self-referenced OCT enables in vivo visualization and quantitative assessment of a distinct hyporeflective band at the Bowman's layer–anterior stroma interface, providing a measurable optical signature of the anterior cornea.

Translational Relevance: Wide-field self-referenced OCT provides noninvasive, in vivo access to a corneal region previously characterized mainly by ex vivo or highly localized methods. This capability enables future studies examining whether the hyporeflective band is altered in corneal disease.

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Cite

BibTeXRIS
BibTeX
@article{ruggeri2026vivo,
  title = {{In Vivo Wide-Field OCT Imaging Reveals and Quantifies a Hyporeflective Band beneath Bowman's Layer}},
  author = {Marco Ruggeri and Yasufumi Tomioka and Ngoc Lan Vy Truong and Carolina Parra Rosas and Elena Veronica Perozzo and Francesco Pozzo Giuffrida and Florence Cabot and Jean-Marie Parel and Chie Sotozono and Shigeru Kinoshita and Victor L. Perez and Arthur Ho and Mohamed Abou-Shousha and Fabrice Manns},
  journal = {Translational Vision Science \& Technology},
  year = {2026},
  month = oct,
  doi = {10.1167/tvst.15.10.33},
  url = {https://doi.org/10.1167/tvst.15.10.33},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - In Vivo Wide-Field OCT Imaging Reveals and Quantifies a Hyporeflective Band beneath Bowman's Layer
AU  - Marco Ruggeri
AU  - Yasufumi Tomioka
AU  - Ngoc Lan Vy Truong
AU  - Carolina Parra Rosas
AU  - Elena Veronica Perozzo
AU  - Francesco Pozzo Giuffrida
AU  - Florence Cabot
AU  - Jean-Marie Parel
AU  - Chie Sotozono
AU  - Shigeru Kinoshita
AU  - Victor L. Perez
AU  - Arthur Ho
AU  - Mohamed Abou-Shousha
AU  - Fabrice Manns
PY  - 2026
DA  - 2026/10/22
JO  - Translational Vision Science & Technology
DO  - 10.1167/tvst.15.10.33
UR  - https://doi.org/10.1167/tvst.15.10.33
AB  - Purpose: To investigate the anterior corneal region immediately beneath Bowman's layer using wide-field self-referenced optical coherence tomography (OCT) and to identify and characterize a distinct hyporeflective band at the Bowman's layer–anterior stroma interface.  Methods: Seventeen eyes from 17 healthy subjects were analyzed with a custom wide-field spectral-domain OCT system in a self-referenced common-path configuration. Eighteen radial scans over a 9-mm corneal diameter were acquired and processed. A segmentation framework was developed to identify the boundaries of the epithelium, Bowman's layer, hyporeflective band, and total cornea. Thickness maps were generated and analyzed across four anatomical zones. Repeatability was assessed using repeated segmentations.  Results: A thin, continuous hyporeflective band (HB) immediately beneath Bowman's layer was consistently visualized across the corneal field in all 17 eyes analyzed. Mean HB thickness was approximately 8 µm, with slightly greater central than peripheral values. However, zonal differences remained within measurement repeatability. Thickness of the epithelium and Bowman's layer showed minimal spatial variation within measurement precision.  Conclusions: Wide-field self-referenced OCT enables in vivo visualization and quantitative assessment of a distinct hyporeflective band at the Bowman's layer–anterior stroma interface, providing a measurable optical signature of the anterior cornea.  Translational Relevance: Wide-field self-referenced OCT provides noninvasive, in vivo access to a corneal region previously characterized mainly by ex vivo or highly localized methods. This capability enables future studies examining whether the hyporeflective band is altered in corneal disease.
SN  - 2164-2591
ER  -