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Peer-reviewedCC BY-NCScience Advances

A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling

  • Peng‐Tao Shi1,2
  • Ran Zhang3
  • Xuan-Jie Wu2
  • Zhe Zhang2
  • Binbin Li4
  • Si-Lin Wang5
  • Hao Chen6
  • Xiu-Ni Fan5
  • Wen-Ting Liu2
  • Jingjing Wen2
  • Kun‐Ming Chen2
6 affiliations
  1. 1Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Qinhuangdao 066600, Hebei, China
  2. 2State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
  3. 3College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  4. 4Institute of Quality Standards and Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, China
  5. 5Hanbin District Agricultural Technology Extension Station, Ankang 725000, Shaanxi, China
  6. 6Qinyeqing Agricultural Development Co. Ltd, Ankang 725000, Shaanxi, China
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Abstract

Respiratory burst oxidase homolog (RBOH)–based ROS production was widely verified playing vital roles in plant stress responses; however, its inhibitory mechanism remains largely unknown. Here, we demonstrate that a calmodulin-like (CML) protein OsCML27 functions as a flexible brake of OsRbohB-mediated ROS production to regulate rice drought tolerance via an OsMRLK63-OsCML27-OsRbohB module. OsCML27 negatively regulates drought tolerance of rice where the overexpression lines showed a faster water loss rate and a lower survival rate under drought, while the oscml27 mutants exhibited a slower dehydration rate and a slightly lower survival rate as compared to wild type. OsCML27 can interact with and be phosphorylated by a receptor-like kinase OsMRLK63 primarily at Ser 11 , and the phosphorylation level could be increased by drought. The phosphorylation by OsMRLK63 enhances OsCML27 stability, and OsCML27 inhibits ROS production by interacting with OsRbohB in the Ca 2+ -dependent manner. All these results reveal that OsCML27 integrates phosphorylation and Ca 2+ signals to regulate drought tolerance of rice by fine-tuning OsRbohB-mediated ROS signaling.

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Cite

BibTeXRIS
BibTeX
@article{shi2026rice,
  title = {{A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling}},
  author = {Peng‐Tao Shi and Ran Zhang and Xuan-Jie Wu and Zhe Zhang and Binbin Li and Si-Lin Wang and Hao Chen and Xiu-Ni Fan and Wen-Ting Liu and Jingjing Wen and Kun‐Ming Chen},
  journal = {Science Advances},
  year = {2026},
  month = jun,
  doi = {10.1126/sciadv.aee6303},
  url = {https://doi.org/10.1126/sciadv.aee6303},
  note = {CC BY-NC},
}
RIS
TY  - JOUR
TI  - A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling
AU  - Peng‐Tao Shi
AU  - Ran Zhang
AU  - Xuan-Jie Wu
AU  - Zhe Zhang
AU  - Binbin Li
AU  - Si-Lin Wang
AU  - Hao Chen
AU  - Xiu-Ni Fan
AU  - Wen-Ting Liu
AU  - Jingjing Wen
AU  - Kun‐Ming Chen
PY  - 2026
DA  - 2026/06/26
JO  - Science Advances
DO  - 10.1126/sciadv.aee6303
UR  - https://doi.org/10.1126/sciadv.aee6303
AB  - Respiratory burst oxidase homolog (RBOH)–based ROS production was widely verified playing vital roles in plant stress responses; however, its inhibitory mechanism remains largely unknown. Here, we demonstrate that a calmodulin-like (CML) protein OsCML27 functions as a flexible brake of OsRbohB-mediated ROS production to regulate rice drought tolerance via an OsMRLK63-OsCML27-OsRbohB module. OsCML27 negatively regulates drought tolerance of rice where the overexpression lines showed a faster water loss rate and a lower survival rate under drought, while the oscml27 mutants exhibited a slower dehydration rate and a slightly lower survival rate as compared to wild type. OsCML27 can interact with and be phosphorylated by a receptor-like kinase OsMRLK63 primarily at Ser 11 , and the phosphorylation level could be increased by drought. The phosphorylation by OsMRLK63 enhances OsCML27 stability, and OsCML27 inhibits ROS production by interacting with OsRbohB in the Ca 2+ -dependent manner. All these results reveal that OsCML27 integrates phosphorylation and Ca 2+ signals to regulate drought tolerance of rice by fine-tuning OsRbohB-mediated ROS signaling.
SN  - 2375-2548
ER  -