Dereplication of Natural Products from an Endophytic Fungus from Ocotea diospyrifolia with Anti-Inflammatory Potential Through Dual Modulation of PGE2 and LTB4 Release
4 affiliations
- 1Department of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador 40170-115, BA, Brazil
- 2Institute of Chemistry, Federal University of Alfenas, Alfenas 37130-001, MG, Brazil
- 3Institute for Research in Pharmaceuticals and Medications, Federal University of Paraíba, Campus I, João Pessoa 58051-900, PB, Brazil
- 4Department of Chemistry, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil
Abstract
Endophytic fungi are significant sources of structurally diverse natural products with promising pharmacological and biological applications. In this study, endophytic fungi isolated from Ocotea diospyrifolia leaves were investigated as potential producers of anti-inflammatory natural products using an integrated workflow combining biological screening, comparative metabolomics, and high-resolution mass spectrometry-based dereplication. Crude extracts from five endophytic fungi (designated as OD1, OD5, OD6, OD7, and OD8) were screened for their ability to inhibit prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) release. Lentinus sp. OD5 extract displayed promising dual inhibitory activity, and its UHPLC-QTOF-HRMS-based metabolomic profiling enabled the annotation of two compounds. Mass spectrometry data analysis, combined with SIRIUS annotation, putatively identified rhodojaponin, a grayanane diterpene with reported anti-inflammatory activity, and tinocordiside, an immunomodulatory diterpenoid glycoside. These findings expand the chemical diversity currently associated with endophytic fungi and provide a strong foundation for the future isolation, structural confirmation, and pharmacological evaluation of anti-inflammatory metabolites with potential therapeutic applications.
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BibTeX
@article{oliveira2026dereplication,
title = {{Dereplication of Natural Products from an Endophytic Fungus from Ocotea diospyrifolia with Anti-Inflammatory Potential Through Dual Modulation of PGE2 and LTB4 Release}},
author = {Giovanni S. Oliveira and Marcus V. A. Marques and Ana L. U. Nascimento and Valmore H. P. Santos and Daniela A. Garcia and Lara P. D. M. Costa and Thiago A. M. Brito and Josean F. Tavares and Jorge M. David and Daniela A. Chagas-Paula and Eliane O. Silva},
journal = {Molecules (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/molecules31193499},
url = {https://europepmc.org/article/PMC/PMC13666672},
note = {CC BY},
}
RIS
TY - JOUR
TI - Dereplication of Natural Products from an Endophytic Fungus from Ocotea diospyrifolia with Anti-Inflammatory Potential Through Dual Modulation of PGE2 and LTB4 Release
AU - Giovanni S. Oliveira
AU - Marcus V. A. Marques
AU - Ana L. U. Nascimento
AU - Valmore H. P. Santos
AU - Daniela A. Garcia
AU - Lara P. D. M. Costa
AU - Thiago A. M. Brito
AU - Josean F. Tavares
AU - Jorge M. David
AU - Daniela A. Chagas-Paula
AU - Eliane O. Silva
PY - 2026
DA - 2026/10/01
JO - Molecules (Basel, Switzerland)
DO - 10.3390/molecules31193499
UR - https://europepmc.org/article/PMC/PMC13666672
AB - Endophytic fungi are significant sources of structurally diverse natural products with promising pharmacological and biological applications. In this study, endophytic fungi isolated from Ocotea diospyrifolia leaves were investigated as potential producers of anti-inflammatory natural products using an integrated workflow combining biological screening, comparative metabolomics, and high-resolution mass spectrometry-based dereplication. Crude extracts from five endophytic fungi (designated as OD1, OD5, OD6, OD7, and OD8) were screened for their ability to inhibit prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) release. Lentinus sp. OD5 extract displayed promising dual inhibitory activity, and its UHPLC-QTOF-HRMS-based metabolomic profiling enabled the annotation of two compounds. Mass spectrometry data analysis, combined with SIRIUS annotation, putatively identified rhodojaponin, a grayanane diterpene with reported anti-inflammatory activity, and tinocordiside, an immunomodulatory diterpenoid glycoside. These findings expand the chemical diversity currently associated with endophytic fungi and provide a strong foundation for the future isolation, structural confirmation, and pharmacological evaluation of anti-inflammatory metabolites with potential therapeutic applications.
SN - 1420-3049
ER -