Temporal and spatial atlas of eosinophil specialization across tissues
Abstract
Eosinophils are multifunctional granulocytes involved in immune regulation, metabolism and tissue repair; however, the extent of their heterogeneity across tissues and the principles governing their specialization are not well defined. Here, we present a single-cell transcriptomic and proteomic atlas of mouse eosinophils spanning immune, barrier and metabolic sites. By integrating transcriptional profiling, high-dimensional surface proteomics and in vivo fate mapping, we show that eosinophil identity is shaped by both tissue-derived cues and the duration of local residency. Long-lived eosinophils in the small intestine diversify into transcriptionally and phenotypically distinct subsets, whereas short-lived and intermediate-lived populations in the lungs and colon, respectively, remain comparatively uniform. We identify trajectory-associated surface markers that stratify eosinophil maturation from bone-marrow progenitors to long-term tissue-resident subsets. This atlas establishes a unified framework in which tissue-specific residency time drives eosinophil maturation and diversification, providing a molecular toolkit for resolving eosinophil states in health and disease.
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BibTeX
@article{hu2026temporal,
title = {{Temporal and spatial atlas of eosinophil specialization across tissues}},
author = {Yanan Hu and Lianfu Wu and Sihao Qu and Wan Ting Kong and Xiaotong Yu and Jiaqian Xu and Ziyi Xu and Jialing He and Chong Wang and Zhaoyuan Liu and Liming Lu and Lai Guan Ng and Camille Blériot and Bing Su and Florent Ginhoux and Ziyi Li and Svetoslav Chakarov},
journal = {Nature Immunology},
year = {2026},
month = oct,
doi = {10.1038/s41590-025-02382-6},
url = {https://hal.science/hal-05762940v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Temporal and spatial atlas of eosinophil specialization across tissues
AU - Yanan Hu
AU - Lianfu Wu
AU - Sihao Qu
AU - Wan Ting Kong
AU - Xiaotong Yu
AU - Jiaqian Xu
AU - Ziyi Xu
AU - Jialing He
AU - Chong Wang
AU - Zhaoyuan Liu
AU - Liming Lu
AU - Lai Guan Ng
AU - Camille Blériot
AU - Bing Su
AU - Florent Ginhoux
AU - Ziyi Li
AU - Svetoslav Chakarov
PY - 2026
DA - 2026/10/26
JO - Nature Immunology
DO - 10.1038/s41590-025-02382-6
UR - https://hal.science/hal-05762940v1
AB - Eosinophils are multifunctional granulocytes involved in immune regulation, metabolism and tissue repair; however, the extent of their heterogeneity across tissues and the principles governing their specialization are not well defined. Here, we present a single-cell transcriptomic and proteomic atlas of mouse eosinophils spanning immune, barrier and metabolic sites. By integrating transcriptional profiling, high-dimensional surface proteomics and in vivo fate mapping, we show that eosinophil identity is shaped by both tissue-derived cues and the duration of local residency. Long-lived eosinophils in the small intestine diversify into transcriptionally and phenotypically distinct subsets, whereas short-lived and intermediate-lived populations in the lungs and colon, respectively, remain comparatively uniform. We identify trajectory-associated surface markers that stratify eosinophil maturation from bone-marrow progenitors to long-term tissue-resident subsets. This atlas establishes a unified framework in which tissue-specific residency time drives eosinophil maturation and diversification, providing a molecular toolkit for resolving eosinophil states in health and disease.
SN - 1529-2908
SN - 1529-2916
ER -