ASSOCIATION BETWEEN INTERFERON-Γ LEVELS IN MOBILIZED HEMATOPOIETIC STEM CELL PRODUCTS AND LOSS OF RESPONSE AFTER AUTOLOGOUS STEM CELL TRANSPLANTATION IN MULTIPLE MYELOMA
- 1Universidade Estadual do Oeste do Paraná (Unioeste), Francisco Beltrão, PR, Brazil
Abstract
Introduction: Cytokine-mediated interactions within the bone marrow microenvironment play a central role in the pathogenesis of multiple myeloma (MM). Although several cytokines have been investigated in peripheral blood and bone marrow, little is known about the prognostic significance of cytokine levels in the leukapheresis product used for autologous stem cell transplantation and their relationship with post-transplant treatment response. Materials and methods: This retrospective cross-sectional study evaluated the concentrations of tumor necrosis factor alpha (TNF-α), transforming growth factor beta 1 (TGF-β1), and interferon gamma (IFN-γ) in peripheral hematopoietic stem cell (PHSC) leukapheresis products obtained from transplant-eligible MM patients. Cytokine levels were correlated with mobilized CD34+ cell yield, disease stage, treatment response, and clinical outcomes. Results: The median baseline IFN-γ concentration was 826.4 pg/mL. Patients who achieved complete response (CR) three months after autologous transplantation had significantly lower IFN-γ levels in the leukapheresis product than those with very good partial response (VGPR) (674.75 ± 80.32 pg/mL vs. 939.6 ± 106.8 pg/mL, p = 0.02). Moreover, patients who experienced loss of response depth at three months post-transplant exhibited markedly higher IFN-γ levels (median: 1,133 pg/mL) than those whose response improved after transplantation (median: 485 pg/mL), characterizing high- and low-IFN-γ expression profiles, respectively. No significant associations were observed between TNF-α, TGF-β1, or IFN-γ levels and overall survival or progression-free survival. Likewise, TNF-α and TGF-β1 concentrations were not associated with post-transplant response. Conclusion: Elevated IFN-γ levels in peripheral hematopoietic stem cell leukapheresis products are associated with early loss of response following autologous stem cell transplantation in patients with multiple myeloma. These findings suggest that IFN-γ may represent a promising biomarker for early post transplant risk stratification and therapeutic decision-making.
Related
- Real-world analysis of thromboprophylaxis use during autologous hematopoietic stem cell transplantation for multiple myeloma
- PREDICTIVE FACTORS FOR HEMATOPOIETIC STEM CELL MOBILIZATION IN AUTOLOGOUS TRANSPLANTATION: DEFINING A PERIPHERAL CD34+ CUTOFF FOR SINGLE-SESSION SUCCESS
- SERUM IL-12 PREDICTS MINIMAL RESIDUAL DISEASE NEGATIVITY AFTER AUTOLOGOUS HEMATOPOIETIC STEM CELL TRANSPLANTATION IN PATIENTS WITH MULTIPLE MYELOMA
- Efficacy and safety of air-flow beds for autologous hematopoietic stem cell transplantation in multiple myeloma: a retrospective cohort study
- Variability of Nutritional Status During Hematopoietic Stem Cell Transplantation and Its Association With Clinical Outcomes: An Ambispective Observational Study From India
BibTeX
@article{cardoso2026association,
title = {{ASSOCIATION BETWEEN INTERFERON-Γ LEVELS IN MOBILIZED HEMATOPOIETIC STEM CELL PRODUCTS AND LOSS OF RESPONSE AFTER AUTOLOGOUS STEM CELL TRANSPLANTATION IN MULTIPLE MYELOMA}},
author = {Laura Gabriela CARDOSO and Letícia NAVARRO GORDAN FERREIRA MARTINS and Ana Luiza GOULART STARCK and Gustavo SCHROLL DOS PRAZERES and Micael DANGUI MENEZES and Geise Ellen BROTO and Carolina PANIS},
journal = {Hematology, Transfusion and Cell Therapy},
year = {2026},
month = oct,
doi = {10.1016/j.htct.2026.106828},
url = {https://doi.org/10.1016/j.htct.2026.106828},
note = {CC BY},
}
RIS
TY - JOUR
TI - ASSOCIATION BETWEEN INTERFERON-Γ LEVELS IN MOBILIZED HEMATOPOIETIC STEM CELL PRODUCTS AND LOSS OF RESPONSE AFTER AUTOLOGOUS STEM CELL TRANSPLANTATION IN MULTIPLE MYELOMA
AU - Laura Gabriela CARDOSO
AU - Letícia NAVARRO GORDAN FERREIRA MARTINS
AU - Ana Luiza GOULART STARCK
AU - Gustavo SCHROLL DOS PRAZERES
AU - Micael DANGUI MENEZES
AU - Geise Ellen BROTO
AU - Carolina PANIS
PY - 2026
DA - 2026/10/23
JO - Hematology, Transfusion and Cell Therapy
DO - 10.1016/j.htct.2026.106828
UR - https://doi.org/10.1016/j.htct.2026.106828
AB - Introduction: Cytokine-mediated interactions within the bone marrow microenvironment play a central role in the pathogenesis of multiple myeloma (MM). Although several cytokines have been investigated in peripheral blood and bone marrow, little is known about the prognostic significance of cytokine levels in the leukapheresis product used for autologous stem cell transplantation and their relationship with post-transplant treatment response. Materials and methods: This retrospective cross-sectional study evaluated the concentrations of tumor necrosis factor alpha (TNF-α), transforming growth factor beta 1 (TGF-β1), and interferon gamma (IFN-γ) in peripheral hematopoietic stem cell (PHSC) leukapheresis products obtained from transplant-eligible MM patients. Cytokine levels were correlated with mobilized CD34+ cell yield, disease stage, treatment response, and clinical outcomes. Results: The median baseline IFN-γ concentration was 826.4 pg/mL. Patients who achieved complete response (CR) three months after autologous transplantation had significantly lower IFN-γ levels in the leukapheresis product than those with very good partial response (VGPR) (674.75 ± 80.32 pg/mL vs. 939.6 ± 106.8 pg/mL, p = 0.02). Moreover, patients who experienced loss of response depth at three months post-transplant exhibited markedly higher IFN-γ levels (median: 1,133 pg/mL) than those whose response improved after transplantation (median: 485 pg/mL), characterizing high- and low-IFN-γ expression profiles, respectively. No significant associations were observed between TNF-α, TGF-β1, or IFN-γ levels and overall survival or progression-free survival. Likewise, TNF-α and TGF-β1 concentrations were not associated with post-transplant response. Conclusion: Elevated IFN-γ levels in peripheral hematopoietic stem cell leukapheresis products are associated with early loss of response following autologous stem cell transplantation in patients with multiple myeloma. These findings suggest that IFN-γ may represent a promising biomarker for early post transplant risk stratification and therapeutic decision-making.
SN - 2531-1379
SN - 2531-1387
ER -