PREDICTIVE FACTORS FOR HEMATOPOIETIC STEM CELL MOBILIZATION IN AUTOLOGOUS TRANSPLANTATION: DEFINING A PERIPHERAL CD34+ CUTOFF FOR SINGLE-SESSION SUCCESS
- 1Rede Américas, São Paulo, SP, Brazil
- 2Grupo Pulsa São Paulo, São Paulo, SP, Brazil
Abstract
Introduction: Autologous hematopoietic stem cell transplantation is a standard treatment for lymphomas and multiple myeloma. Efficient mobilization and collection of CD34+ cells are essential to reduce costs, procedure time, and patient burden. Identifying predictors of successful collection in a single leukapheresis session is particularly relevant in resource-limited settings. Casuistic: This study included 103 adult patients undergoing stem cell mobilization and collection between 2019 and 2023 at a tertiary center. The cohort comprised 54.4% men, with a mean age of 54.6 years. Indications were lymphomas (48.5%), multiple myeloma (47.6%), and other plasma cell disorders (3.9%). Methodology: A retrospective, single-center study was conducted using clinical, laboratory, and procedural data. Mobilization was performed with G-CSF alone or combined with plerixafor. The primary endpoint was successful collection in a single leukapheresis session, defined as ≥ 2 × 10⁶ CD34+ cells/kg. ROC curve analysis determined the optimal peripheral CD34+ cutoff. Factors associated with the outcome were assessed using univariate and multivariate analyses. Results: A total of 149 leukapheresis procedures were performed. Single-session success was achieved in 61.2% of patients. The mean peripheral CD34+ count on the first collection day was 39 cells/mm³. A cutoff > 33 cells/mm³ predicted successful single-session collection. In multivariate analysis, the use of G-CSF plus plerixafor (OR 2.48; p = 0.045) and higher leukocyte count on the collection day (OR 2.48; p = 0.030) were independently associated with success. The mean CD34+ yield on the first day was 4.4 × 10⁶ cells/kg, with viability > 99%. The mean engraftment time was 11.4 days. Conclusions: A peripheral CD34+ count > 33 cells/mm³ is a robust predictor of successful single session leukapheresis. The addition of plerixafor and higher leukocyte counts improve mobilization outcomes. These findings support more efficient and individualized mobilization strategies, optimizing resource utilization and improving patient outcomes.
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BibTeX
@article{reichert2026predictive,
title = {{PREDICTIVE FACTORS FOR HEMATOPOIETIC STEM CELL MOBILIZATION IN AUTOLOGOUS TRANSPLANTATION: DEFINING A PERIPHERAL CD34+ CUTOFF FOR SINGLE-SESSION SUCCESS}},
author = {Breno Aires Cadiele Oliana REICHERT and Celso ARRAIS and Raquel Valverde SOUSA and Renata Priscila GOMES and Waldely Veneziani CALHEIROS and Erica Mioto Braz MERCHAN and Mirianceli Coelho MENDONÇA and Cesar De Almeida NETO},
journal = {Hematology, Transfusion and Cell Therapy},
year = {2026},
month = oct,
doi = {10.1016/j.htct.2026.106900},
url = {https://doi.org/10.1016/j.htct.2026.106900},
note = {CC BY},
}
RIS
TY - JOUR
TI - PREDICTIVE FACTORS FOR HEMATOPOIETIC STEM CELL MOBILIZATION IN AUTOLOGOUS TRANSPLANTATION: DEFINING A PERIPHERAL CD34+ CUTOFF FOR SINGLE-SESSION SUCCESS
AU - Breno Aires Cadiele Oliana REICHERT
AU - Celso ARRAIS
AU - Raquel Valverde SOUSA
AU - Renata Priscila GOMES
AU - Waldely Veneziani CALHEIROS
AU - Erica Mioto Braz MERCHAN
AU - Mirianceli Coelho MENDONÇA
AU - Cesar De Almeida NETO
PY - 2026
DA - 2026/10/23
JO - Hematology, Transfusion and Cell Therapy
DO - 10.1016/j.htct.2026.106900
UR - https://doi.org/10.1016/j.htct.2026.106900
AB - Introduction: Autologous hematopoietic stem cell transplantation is a standard treatment for lymphomas and multiple myeloma. Efficient mobilization and collection of CD34+ cells are essential to reduce costs, procedure time, and patient burden. Identifying predictors of successful collection in a single leukapheresis session is particularly relevant in resource-limited settings. Casuistic: This study included 103 adult patients undergoing stem cell mobilization and collection between 2019 and 2023 at a tertiary center. The cohort comprised 54.4% men, with a mean age of 54.6 years. Indications were lymphomas (48.5%), multiple myeloma (47.6%), and other plasma cell disorders (3.9%). Methodology: A retrospective, single-center study was conducted using clinical, laboratory, and procedural data. Mobilization was performed with G-CSF alone or combined with plerixafor. The primary endpoint was successful collection in a single leukapheresis session, defined as ≥ 2 × 10⁶ CD34+ cells/kg. ROC curve analysis determined the optimal peripheral CD34+ cutoff. Factors associated with the outcome were assessed using univariate and multivariate analyses. Results: A total of 149 leukapheresis procedures were performed. Single-session success was achieved in 61.2% of patients. The mean peripheral CD34+ count on the first collection day was 39 cells/mm³. A cutoff > 33 cells/mm³ predicted successful single-session collection. In multivariate analysis, the use of G-CSF plus plerixafor (OR 2.48; p = 0.045) and higher leukocyte count on the collection day (OR 2.48; p = 0.030) were independently associated with success. The mean CD34+ yield on the first day was 4.4 × 10⁶ cells/kg, with viability > 99%. The mean engraftment time was 11.4 days. Conclusions: A peripheral CD34+ count > 33 cells/mm³ is a robust predictor of successful single session leukapheresis. The addition of plerixafor and higher leukocyte counts improve mobilization outcomes. These findings support more efficient and individualized mobilization strategies, optimizing resource utilization and improving patient outcomes.
SN - 2531-1379
SN - 2531-1387
ER -