Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors
Abstract
Endocrine disrupting chemicals (EDCs) play a part in the modern burst of diseases and interfere with the steroid hormone axis. Bisphenol A (BPA), one of the most active and widely used EDCs, affects ameloblast functions, leading to an enamel hypomineralization pattern similar to that of Molar Incisor Hypomineralization (MIH). In order to explore the molecular pathways stimulated by BPA during amelogenesis, we thoroughly investigated the receptors known to directly or indirectly mediate the effects of BPA. The expression patterns of high affinity BPA receptors (ERRγ, GPR30), of ketosteroid receptors (ERs, AR, PGR, GR, MR), of the retinoid receptor RXRα and PPARγ were established using RT-qPCR analysis of RNAs extracted from microdissected enamel organ of adult rats. Their expression was dependent on the stage of ameloblast differentiation, except that of ERβ and PPARγ which remained undetectable. An additional large scale microarray analysis revealed three main groups of receptors according to their level of expression in maturation stage ameloblasts. The expression level of RXRα was the highest, similar to the vitamin D receptor (VDR), whereas the others were 13 to 612 fold lower, with AR and GR being intermediate. Immunohistological analysis of VDR and AR confirmed their presence mainly in maturation- stage ameloblasts. All These data provide further evidence the fact that ameloblasts express a specific combination of hormonal receptors depending on their developmental stage. This study represents the first step towards understanding dental endocrinology as well as some of the effects of EDCs on the pathophysiology of amelogenesis.
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BibTeX
@article{houari2026expression,
title = {{Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors}},
author = {Sophia Houari and Sophia Loiodice and Katia Jedeon and Ariane Berdal and Sylvie Babajko},
journal = {Frontiers in Physiology},
year = {2026},
month = sep,
doi = {10.3389/fphys.2016.00503},
url = {https://hal.science/hal-04199213v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors
AU - Sophia Houari
AU - Sophia Loiodice
AU - Katia Jedeon
AU - Ariane Berdal
AU - Sylvie Babajko
PY - 2026
DA - 2026/09/24
JO - Frontiers in Physiology
DO - 10.3389/fphys.2016.00503
UR - https://hal.science/hal-04199213v1
AB - Endocrine disrupting chemicals (EDCs) play a part in the modern burst of diseases and interfere with the steroid hormone axis. Bisphenol A (BPA), one of the most active and widely used EDCs, affects ameloblast functions, leading to an enamel hypomineralization pattern similar to that of Molar Incisor Hypomineralization (MIH). In order to explore the molecular pathways stimulated by BPA during amelogenesis, we thoroughly investigated the receptors known to directly or indirectly mediate the effects of BPA. The expression patterns of high affinity BPA receptors (ERRγ, GPR30), of ketosteroid receptors (ERs, AR, PGR, GR, MR), of the retinoid receptor RXRα and PPARγ were established using RT-qPCR analysis of RNAs extracted from microdissected enamel organ of adult rats. Their expression was dependent on the stage of ameloblast differentiation, except that of ERβ and PPARγ which remained undetectable. An additional large scale microarray analysis revealed three main groups of receptors according to their level of expression in maturation stage ameloblasts. The expression level of RXRα was the highest, similar to the vitamin D receptor (VDR), whereas the others were 13 to 612 fold lower, with AR and GR being intermediate. Immunohistological analysis of VDR and AR confirmed their presence mainly in maturation- stage ameloblasts. All These data provide further evidence the fact that ameloblasts express a specific combination of hormonal receptors depending on their developmental stage. This study represents the first step towards understanding dental endocrinology as well as some of the effects of EDCs on the pathophysiology of amelogenesis.
SN - 1664-042X
ER -