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Peer-reviewedCC BY 4.0PLOS One

Development of shelf-stable reagents and assay kits for bioluminescence applications using the capillary-assisted vitrification platform stabilization technology

  • Shari Radford1
  • Yolanda Peris-Taverner2
  • Michael Dibble3
  • Kevin Corn4
  • Tian Zhu5
  • Shannon Martello
  • McKenzie Mayeaux
  • Amanda Ladd
  • Sankar Renu
  • Tejasvi Chunduri
  • Aniket Jadhav
  • Melanie Dart
3 more authors
  • Marjan Rafat
  • Mary Shank-Retzlaff
  • Laura L. Bronsart
5 affiliations
  1. 1Ambient Biosciences, Ann Arbor, Michigan, United States of America
  2. 2Promega Corp., Madison, Wisconsin, United States of America
  3. 3Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, United States of America
  4. 4Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, United States of America
  5. 5Department of Radiation Oncology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
Open at sourceDOICite

Abstract

Luminescence is a powerful method for detecting trace analytes and monitoring biological processes. However, most bioluminescence reagents, including luciferase and its substrates, are sensitive to temperature, limiting their usable shelf lives, and resulting in inconsistent performance. Enhancing the stability of these reagents could reduce signal variability between experiments, simplify workflows, and address cold chain storage issues. In this study, we demonstrate the application of the platform stabilization technology, capillary-assisted vitrification (CAV), as a tool to stabilize different luciferases and their substrates, and the application of the stabilized reagents in both in vitro and in vivo bioluminescent assays. We demonstrate that CAV-stabilized reagents can be stored and shipped ambiently, maintain consistent performance over time, and are suitable for use in cell viability quantification, tumor monitoring, in vivo imaging, microbial detection, and immunoassays. Additionally, different reagents can be co-formulated to make ready-to-use assay kits that can also be shipped and stored ambiently. Our results demonstrate that CAV stabilization is a viable alternative to traditional storage methods, with broad potential to improve bioluminescence workflows.

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Cite

BibTeXRIS
BibTeX
@article{radford2026development,
  title = {{Development of shelf-stable reagents and assay kits for bioluminescence applications using the capillary-assisted vitrification platform stabilization technology}},
  author = {Shari Radford and Yolanda Peris-Taverner and Michael Dibble and Kevin Corn and Tian Zhu and Shannon Martello and McKenzie Mayeaux and Amanda Ladd and Sankar Renu and Tejasvi Chunduri and Aniket Jadhav and Melanie Dart and Marjan Rafat and Mary Shank-Retzlaff and Laura L. Bronsart},
  journal = {PLOS One},
  year = {2026},
  month = nov,
  doi = {10.1371/journal.pone.0354533},
  url = {https://doi.org/10.1371/journal.pone.0354533},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Development of shelf-stable reagents and assay kits for bioluminescence applications using the capillary-assisted vitrification platform stabilization technology
AU  - Shari Radford
AU  - Yolanda Peris-Taverner
AU  - Michael Dibble
AU  - Kevin Corn
AU  - Tian Zhu
AU  - Shannon Martello
AU  - McKenzie Mayeaux
AU  - Amanda Ladd
AU  - Sankar Renu
AU  - Tejasvi Chunduri
AU  - Aniket Jadhav
AU  - Melanie Dart
AU  - Marjan Rafat
AU  - Mary Shank-Retzlaff
AU  - Laura L. Bronsart
PY  - 2026
DA  - 2026/11/05
JO  - PLOS One
DO  - 10.1371/journal.pone.0354533
UR  - https://doi.org/10.1371/journal.pone.0354533
AB  - Luminescence is a powerful method for detecting trace analytes and monitoring biological processes. However, most bioluminescence reagents, including luciferase and its substrates, are sensitive to temperature, limiting their usable shelf lives, and resulting in inconsistent performance. Enhancing the stability of these reagents could reduce signal variability between experiments, simplify workflows, and address cold chain storage issues. In this study, we demonstrate the application of the platform stabilization technology, capillary-assisted vitrification (CAV), as a tool to stabilize different luciferases and their substrates, and the application of the stabilized reagents in both in vitro and in vivo bioluminescent assays. We demonstrate that CAV-stabilized reagents can be stored and shipped ambiently, maintain consistent performance over time, and are suitable for use in cell viability quantification, tumor monitoring, in vivo imaging, microbial detection, and immunoassays. Additionally, different reagents can be co-formulated to make ready-to-use assay kits that can also be shipped and stored ambiently. Our results demonstrate that CAV stabilization is a viable alternative to traditional storage methods, with broad potential to improve bioluminescence workflows.
SN  - 1932-6203
ER  -