Nanostructured hybrid materials for bone-tooth unit regeneration

Abstract

As a part of regenerative medicine, biomaterials are largely used in this field of nanotechnology and tissue engineering research. We have recently developed a new scaffold using electrospun nanofibers of Poly (ε-caprolactone), PCL which is able to mimic the collagen extracellular matrix of cells. The aim of this study was to engineer a biological and implantable structure leading the regeneration of the tooth-bone unit. For this aim, we have cultured mouse osteoblasts embedded in a collagen gel on the nanofibrous membrane and coupled this structure with an embryonic dental germ before implantation. To follow bone and tooth regeneration, we have performed RT-PCR, histology and immunofluorescence analysis. We showed here that this leaving implantable structure represents an accurate strategy for bone-tooth unit regeneration. We report here the first demonstration of bone-tooth unit regeneration by using a strategy based on a synthetic nanostructured membrane. This electrospun membrane is manufactured by using an FDA approved polymer, PCL and functionalized with osteoblasts before incorporation of the tooth germs at ED14 (the first lower molars) to generate bone-tooth unit in vivo after implantation in mice. Our technology represents an excellent platform on which other sophisticated products could be based.

Coupled FE Modeling of Superelastic NiTi Arch Wire and Bone Remodeling to Simulate 3D Orthodontic Tooth Movement During Leveling Phase
Mouhamad Abou Hamdan, Delphine Wagner, Hamdi Jmal +1
International Journal for Numerical Methods in Biomedical Engineering29 Sep 2026Peer-reviewedCC BY 4.0Full textSystems & synthetic biologyHAL
A neurovascularized bone regeneration strategy for mandibular and alveolar bone defects based on elastin-like biomaterials
Nadia Mahmoudi, Romane Lesieur, Sylvie Rey +5
Regenerative Biomaterials10 Sep 2026Peer-reviewedCC BY 4.0Full textGeneral biologyHAL
An aged human bone marrow mesenchymal niche causes impaired function of human hematopoietic stem cells under regeneration-induced stress
A. Grigoryan, R. Abdelrahman, W. S. Seo +10
bioRxiv27 Oct 2026PreprintCC BYFull textCell biologybioRxiv
Personalised prediction of anomalous bone regeneration in multiple myeloma patients
F. Gibson, R. E. Andrews, M. A. Lawson +10
bioRxiv26 Oct 2026PreprintCC BYFull textSystems & synthetic biologybioRxiv
Biomaterial-based local delivery of anti-inflammatory drugs, cytokines, and growth factors for bone regeneration
Jiaming Shi, Jiaming Shi, Jiahe Liu +6
Frontiers in Bioengineering and Biotechnology23 Oct 2026Peer-reviewedCC BYFull textSystems & synthetic biologyDOAJ

Cite

BibTeX RIS

BibTeX

@article{kuchlerbopp2026nanostructured,
  title = {{Nanostructured hybrid materials for bone-tooth unit regeneration}},
  author = {Sabine Kuchler-Bopp and Thibault Bécavin and Tunay Kökten and Florence Fioretti and Etienne Deveaux and Nadia Benkirane-Jessel and Laetitia Keller},
  journal = {Open Journal of Regenerative Medicine},
  year = {2026},
  month = sep,
  doi = {10.4236/ojrm.2013.23008},
  url = {https://hal.univ-lorraine.fr/hal-05753344v1},
  note = {CC BY},
}

RIS

TY  - JOUR
TI  - Nanostructured hybrid materials for bone-tooth unit regeneration
AU  - Sabine Kuchler-Bopp
AU  - Thibault Bécavin
AU  - Tunay Kökten
AU  - Florence Fioretti
AU  - Etienne Deveaux
AU  - Nadia Benkirane-Jessel
AU  - Laetitia Keller
PY  - 2026
DA  - 2026/09/17
JO  - Open Journal of Regenerative Medicine
DO  - 10.4236/ojrm.2013.23008
UR  - https://hal.univ-lorraine.fr/hal-05753344v1
AB  - As a part of regenerative medicine, biomaterials are largely used in this field of nanotechnology and tissue engineering research. We have recently developed a new scaffold using electrospun nanofibers of Poly (ε-caprolactone), PCL which is able to mimic the collagen extracellular matrix of cells. The aim of this study was to engineer a biological and implantable structure leading the regeneration of the tooth-bone unit. For this aim, we have cultured mouse osteoblasts embedded in a collagen gel on the nanofibrous membrane and coupled this structure with an embryonic dental germ before implantation. To follow bone and tooth regeneration, we have performed RT-PCR, histology and immunofluorescence analysis. We showed here that this leaving implantable structure represents an accurate strategy for bone-tooth unit regeneration. We report here the first demonstration of bone-tooth unit regeneration by using a strategy based on a synthetic nanostructured membrane. This electrospun membrane is manufactured by using an FDA approved polymer, PCL and functionalized with osteoblasts before incorporation of the tooth germs at ED14 (the first lower molars) to generate bone-tooth unit in vivo after implantation in mice. Our technology represents an excellent platform on which other sophisticated products could be based.
SN  - 2169-2513
SN  - 2169-2521
ER  -

Abstract

Related

Cite