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Peer-reviewedCC BYMicroPublication biology

The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions.

  • Jade Tt Palmer1
  • Monica L Gerth1
  1. 1School of Biological Sciences, Victoria University of Wellington, New Zealand.
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Abstract

Surveillance of Phytophthora agathidicida , the causal agent of kauri dieback, has included soil baiting followed by loop-mediated isothermal amplification (LAMP)-based detection. This approved assay was reported to detect 1 fg of DNA using OptiGene Isothermal Master Mix on a BioRanger instrument. We evaluated its analytical robustness using the same master mix on an alternative platform (QuantStudio 3), and separately tested an alternative NEB master mix. Both assays yielded a reliable detection limit of 1,000 fg, with variable detection at 100 fg and none below. Diagnostic laboratories should verify assay performance under their operational conditions prior to implementation.

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Cite

BibTeXRIS
BibTeX
@article{palmer2026limit,
  title = {{The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions.}},
  author = {Jade Tt Palmer and Monica L Gerth},
  journal = {MicroPublication biology},
  year = {2026},
  month = sep,
  doi = {10.17912/micropub.biology.002163},
  url = {https://europepmc.org/article/MED/42871916},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions.
AU  - Jade Tt Palmer
AU  - Monica L Gerth
PY  - 2026
DA  - 2026/09/29
JO  - MicroPublication biology
DO  - 10.17912/micropub.biology.002163
UR  - https://europepmc.org/article/MED/42871916
AB  - Surveillance of Phytophthora agathidicida , the causal agent of kauri dieback, has included soil baiting followed by loop-mediated isothermal amplification (LAMP)-based detection. This approved assay was reported to detect 1 fg of DNA using OptiGene Isothermal Master Mix on a BioRanger instrument. We evaluated its analytical robustness using the same master mix on an alternative platform (QuantStudio 3), and separately tested an alternative NEB master mix. Both assays yielded a reliable detection limit of 1,000 fg, with variable detection at 100 fg and none below. Diagnostic laboratories should verify assay performance under their operational conditions prior to implementation.
SN  - 2578-9430
ER  -