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Peer-reviewedCC BY 4.0Animal Bioscience

Putative proteomic signatures associated with post-thaw semen quality in Peranakan Etawah and Saanen goats: an exploratory study

  • Muhammad Ihsan Andi Dagong1
  • Muhammad Yusuf1
  • Athhar Manabi Diansyah1
  • Procula Rudlof Matitaputty2
  • Paskah Partogi Agung3
  • Indra Wahyudi Syarif4
  • Muhammad Fajar Amrullah3,5
5 affiliations
  1. 1Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
  2. 2Research Center for Animal Husbandry, Research Organization for Agriculture and Food, Bogor, Indonesia
  3. 3Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, Indonesia
  4. 4Graduate Student of Animal Production and Technology Science, Graduate School, IPB University, Bogor, Indonesia
  5. 5Graduate Student of Animal Biomedical Science Study Program, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, Indonesia
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Abstract

Objective: The present study aimed to investigate post-thaw semen quality and sperm kinematics between Peranakan Etawah (PE) and Saanen goats, and to identify breed-associated proteomic markers related to semen cryotolerance.

Methods: Frozen semen straws were obtained from BIB Singosari, Indonesia, and grouped by breed. Post-thaw evaluations included cellular viability, morphology/abnormality, plasma membrane integrity (HOST), and computer-assisted sperm analysis for motility and kinematic parameters. Sperm proteomes were profiled via UHPLC–Orbitrap LC–MS/MS. Data were processed in Proteome Discoverer and analyzed using multivariate models (principal component analysis [PCA], partial least squares–discriminant analysis [PLS-DA]), differential abundance testing (q-values), functional enrichment (g:Profiler), biomarker selection (variable importance in projection>1; area under the curve≥0.70), and correlation with semen traits (MetaboAnalyst).

Results: PE and Saanen differed significantly in survival-related traits, including viability, abnormalities, and plasma membrane integrity (p<0.05), while total motility and other kinematic parameters were similar between breeds (p>0.05). Proteomic patterns showed partial separation by PCA and more distinct differentiation through verified PLS-DA. Multiple proteins exhibited variable abundance between the breeds (q<0.05) and clustered reliably by breed. Enrichment analysis highlighted mitochondrion-associated functions, particularly fatty acid β-oxidation. Identified discriminative proteins included ACADVL, ETFB, GPD2, CYLC1, and LOC102188264, which were significantly associated with semen quality traits within breeds.

Conclusion: Differences across breeds in frozen–thawed goat semen were mostly evident in post-thaw viability traits and in proteomic signatures predominantly influenced by mitochondrial energy metabolism. Integrating proteomics with conventional semen assessment may elucidate semen cryotolerance and support biomarker identification.

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Cite

BibTeXRIS
BibTeX
@article{dagong2026putative,
  title = {{Putative proteomic signatures associated with post-thaw semen quality in Peranakan Etawah and Saanen goats: an exploratory study}},
  author = {Muhammad Ihsan Andi Dagong and Muhammad Yusuf and Athhar Manabi Diansyah and Procula Rudlof Matitaputty and Paskah Partogi Agung and Indra Wahyudi Syarif and Muhammad Fajar Amrullah},
  journal = {Animal Bioscience},
  year = {2026},
  month = jul,
  doi = {10.5713/ab.260268},
  url = {https://doi.org/10.5713/ab.260268},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Putative proteomic signatures associated with post-thaw semen quality in Peranakan Etawah and Saanen goats: an exploratory study
AU  - Muhammad Ihsan Andi Dagong
AU  - Muhammad Yusuf
AU  - Athhar Manabi Diansyah
AU  - Procula Rudlof Matitaputty
AU  - Paskah Partogi Agung
AU  - Indra Wahyudi Syarif
AU  - Muhammad Fajar Amrullah
PY  - 2026
DA  - 2026/07/27
JO  - Animal Bioscience
DO  - 10.5713/ab.260268
UR  - https://doi.org/10.5713/ab.260268
AB  - Objective: The present study aimed to investigate post-thaw semen quality and sperm kinematics between Peranakan Etawah (PE) and Saanen goats, and to identify breed-associated proteomic markers related to semen cryotolerance.  Methods: Frozen semen straws were obtained from BIB Singosari, Indonesia, and grouped by breed. Post-thaw evaluations included cellular viability, morphology/abnormality, plasma membrane integrity (HOST), and computer-assisted sperm analysis for motility and kinematic parameters. Sperm proteomes were profiled via UHPLC–Orbitrap LC–MS/MS. Data were processed in Proteome Discoverer and analyzed using multivariate models (principal component analysis [PCA], partial least squares–discriminant analysis [PLS-DA]), differential abundance testing (q-values), functional enrichment (g:Profiler), biomarker selection (variable importance in projection>1; area under the curve≥0.70), and correlation with semen traits (MetaboAnalyst).  Results: PE and Saanen differed significantly in survival-related traits, including viability, abnormalities, and plasma membrane integrity (p<0.05), while total motility and other kinematic parameters were similar between breeds (p>0.05). Proteomic patterns showed partial separation by PCA and more distinct differentiation through verified PLS-DA. Multiple proteins exhibited variable abundance between the breeds (q<0.05) and clustered reliably by breed. Enrichment analysis highlighted mitochondrion-associated functions, particularly fatty acid β-oxidation. Identified discriminative proteins included ACADVL, ETFB, GPD2, CYLC1, and LOC102188264, which were significantly associated with semen quality traits within breeds.  Conclusion: Differences across breeds in frozen–thawed goat semen were mostly evident in post-thaw viability traits and in proteomic signatures predominantly influenced by mitochondrial energy metabolism. Integrating proteomics with conventional semen assessment may elucidate semen cryotolerance and support biomarker identification.
SN  - 2765-0189
SN  - 2765-0235
ER  -