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PreprintCC BY-NCbioRxiv

DECAPPING PROTEIN 5 regulates the circadian clock in Arabidopsis

  • D. A. Careno
  • S. Perez Santangelo
  • J. L. L. Mateos
  • L. Bianchimano
  • H. Pallares
  • D. Jacques-Vuarambon
  • M. S. Shalom
  • A. San Martin
  • M. J. de Leone
  • V. D. Aldas Bulos
  • A. Treichel
  • M. Lewinski
4 more authors
  • R. Siqueira Reis
  • D. Staiger
  • A. A. Bazzini
  • M. J. Yanovsky1
  1. 1Fundacion Instituto Leloir, Instituto de Investigaciones Bioquimicas de Buenos Aires (CONICET), Buenos Aires, Argentina
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Abstract

The Arabidopsis transcriptome must be continuously reshaped to integrate endogenous and environmental cues, including the circadian clock and ambient light. This reprogramming relies on the coordinated synthesis and degradation of mRNAs, yet the contribution of RNA decay factors to circadian regulation remains poorly characterized. Here, we show that nearly all core clock genes are high-amplitude clock-controlled genes (HA-CCGs). A meta-analysis of transcriptome-wide mRNA decay datasets revealed that HA-CCGs have consistently shorter half-lives across multiple studies. We further identify the mRNA decapping activator DECAPPING PROTEIN 5 (DCP5) as a key regulator of circadian RNA stability. Using iCLIP, we show that DCP5 binds preferentially to HA-CCG transcripts at AU-rich motifs in their 3' UTRs, thereby modulating their stability. Accordingly, the dcp5 mutant displays an extended circadian period at both physiological and molecular levels. Our findings establish mRNA decay as a post-transcriptional regulatory layer that fine-tunes the decay of multiple key transcripts within the circadian system.

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Cite

BibTeXRIS
BibTeX
@misc{careno2026decapping,
  title = {{DECAPPING PROTEIN 5 regulates the circadian clock in Arabidopsis}},
  author = {D. A. Careno and S. Perez Santangelo and J. L. L. Mateos and L. Bianchimano and H. Pallares and D. Jacques-Vuarambon and M. S. Shalom and A. San Martin and M. J. de Leone and V. D. Aldas Bulos and A. Treichel and M. Lewinski and R. Siqueira Reis and D. Staiger and A. A. Bazzini and M. J. Yanovsky},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.30.763074},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.30.763074v1},
  note = {CC BY-NC},
}
RIS
TY  - UNPB
TI  - DECAPPING PROTEIN 5 regulates the circadian clock in Arabidopsis
AU  - D. A. Careno
AU  - S. Perez Santangelo
AU  - J. L. L. Mateos
AU  - L. Bianchimano
AU  - H. Pallares
AU  - D. Jacques-Vuarambon
AU  - M. S. Shalom
AU  - A. San Martin
AU  - M. J. de Leone
AU  - V. D. Aldas Bulos
AU  - A. Treichel
AU  - M. Lewinski
AU  - R. Siqueira Reis
AU  - D. Staiger
AU  - A. A. Bazzini
AU  - M. J. Yanovsky
PY  - 2026
DA  - 2026/10/30
PB  - bioRxiv
DO  - 10.64898/2026.10.30.763074
UR  - https://www.biorxiv.org/content/10.64898/2026.10.30.763074v1
AB  - The Arabidopsis transcriptome must be continuously reshaped to integrate endogenous and environmental cues, including the circadian clock and ambient light. This reprogramming relies on the coordinated synthesis and degradation of mRNAs, yet the contribution of RNA decay factors to circadian regulation remains poorly characterized. Here, we show that nearly all core clock genes are high-amplitude clock-controlled genes (HA-CCGs). A meta-analysis of transcriptome-wide mRNA decay datasets revealed that HA-CCGs have consistently shorter half-lives across multiple studies. We further identify the mRNA decapping activator DECAPPING PROTEIN 5 (DCP5) as a key regulator of circadian RNA stability. Using iCLIP, we show that DCP5 binds preferentially to HA-CCG transcripts at AU-rich motifs in their 3' UTRs, thereby modulating their stability. Accordingly, the dcp5 mutant displays an extended circadian period at both physiological and molecular levels. Our findings establish mRNA decay as a post-transcriptional regulatory layer that fine-tunes the decay of multiple key transcripts within the circadian system.
ER  -