A method for determining the content of coumarins in sweet clover seeds
- 1N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Abstract
Introduction. Melilotus Mill. is a valuable legume crop with a great phytomeliorative potential. The coumarins contained in plants help them to overcome adverse environmental conditions, however, phenolic compounds, which have a bitter taste and a specific odor, reduce the palatability of sweet clover feeds. One of the most important directions in sweet clover breeding is the creation of cultivars with a specific coumarin content, which determines their further use. Selection of initial material for this trait requires easily reproducible laboratory methods for determining coumarins in plant raw materials. Seeds of six Melilotus accesssions from the VIR collection were studied. A modified method of spectrophotometric determination of coumarins content in alcohol extraction from crushed seeds was applied. The quantitative determination of coumarins was made on a UV-3000 spectrophotometer (China) at a wavelength of 310 nm. The coumarins content was calculated on the basis of a calibration curve constructed using coumarin solutions of different concentrations. Conclusions. A method for isolating and quantifying total coumarins in the seeds of six Melilotus accessions belonging to five different species has been developed and tested.
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BibTeX
@article{zakharchevnyy2026method,
title = {{A method for determining the content of coumarins in sweet clover seeds}},
author = {I. G. Zakharchevnyy and V. S. Popov and O. V. Duk and L. L. Malyshev},
journal = {Биотехнология и селекция растений},
year = {2026},
month = sep,
doi = {10.30901/2658-6266-2026-3-o7},
url = {https://doi.org/10.30901/2658-6266-2026-3-o7},
note = {CC BY},
}
RIS
TY - JOUR
TI - A method for determining the content of coumarins in sweet clover seeds
AU - I. G. Zakharchevnyy
AU - V. S. Popov
AU - O. V. Duk
AU - L. L. Malyshev
PY - 2026
DA - 2026/09/01
JO - Биотехнология и селекция растений
DO - 10.30901/2658-6266-2026-3-o7
UR - https://doi.org/10.30901/2658-6266-2026-3-o7
AB - Introduction. Melilotus Mill. is a valuable legume crop with a great phytomeliorative potential. The coumarins contained in plants help them to overcome adverse environmental conditions, however, phenolic compounds, which have a bitter taste and a specific odor, reduce the palatability of sweet clover feeds. One of the most important directions in sweet clover breeding is the creation of cultivars with a specific coumarin content, which determines their further use. Selection of initial material for this trait requires easily reproducible laboratory methods for determining coumarins in plant raw materials. Seeds of six Melilotus accesssions from the VIR collection were studied. A modified method of spectrophotometric determination of coumarins content in alcohol extraction from crushed seeds was applied. The quantitative determination of coumarins was made on a UV-3000 spectrophotometer (China) at a wavelength of 310 nm. The coumarins content was calculated on the basis of a calibration curve constructed using coumarin solutions of different concentrations. Conclusions. A method for isolating and quantifying total coumarins in the seeds of six Melilotus accessions belonging to five different species has been developed and tested.
SN - 2658-6266
SN - 2658-6258
ER -