Peer-reviewedCC BY-NCHayati Journal of Biosciences30 Oct 2026

Synthesis, Characterization, and Antibacterial Efficacy of Cu-ZIF-8: In Vitro Study of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria

Nibras Saleh Ghafil Al Tameemi¹
Iman Fadhil Abdul-Husin²
Raid Razzaq Ojaimi³
Zainab Zaidan Mutashar²

¹Ministry of Education, Karbala Education Directorate, First Motafawiqaat Secondary School
²Department of Applied Biotechnology, College of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq
³Department of Medical Biotechnology, College of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq

Abstract

Recently, Staphylococcus aureus and Pseudomonas aeruginosa have become multidrug-resistant (MDR) and produce many virulence factors, posing a health risk. Therefore, addressing knowledge gaps and finding solutions to the challenge caused by bacteria's increased resistance to multiple antibiotics is an urgent need. To address this risk, we aim to synthesize new nanoparticles with long-lasting, safe effects and low toxicity. Metal–organic frameworks (MOFs) are promising materials with high efficiency in inhibiting or killing MDR bacteria. In this investigation, we successfully synthesized copper-doped ZIF-8 (Cu-ZIF-8) nanocomposites using the solvothermal method. Its characteristics were studied using techniques like SEM, FTIR, EDX, and XRD. The Cu-ZIF-8-MOF exhibited high antibacterial efficacy against S. aureus (Gram-positive) and P. aeruginosa (Gram-negative), with concentrations of 12.5, 25, 50, and 100 μg/mL showing high efficiency against P. aeruginosa. In contrast, it was less effective against S. aureus. These results open up wide prospects for the application of the Cu-ZIF-8-MOF antimicrobial agent.

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Cite

BibTeX RIS

BibTeX

@article{tameemi2026synthesis,
  title = {{Synthesis, Characterization, and Antibacterial Efficacy of Cu-ZIF-8: In Vitro Study of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria}},
  author = {Nibras Saleh Ghafil Al Tameemi and Iman Fadhil Abdul-Husin and Raid Razzaq Ojaimi and Zainab Zaidan Mutashar},
  journal = {Hayati Journal of Biosciences},
  year = {2026},
  month = oct,
  doi = {10.4308/hjb.33.6.1541-1551},
  url = {https://doi.org/10.4308/hjb.33.6.1541-1551},
  note = {CC BY-NC},
}

RIS

TY  - JOUR
TI  - Synthesis, Characterization, and Antibacterial Efficacy of Cu-ZIF-8: In Vitro Study of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria
AU  - Nibras Saleh Ghafil Al Tameemi
AU  - Iman Fadhil Abdul-Husin
AU  - Raid Razzaq Ojaimi
AU  - Zainab Zaidan Mutashar
PY  - 2026
DA  - 2026/10/30
JO  - Hayati Journal of Biosciences
DO  - 10.4308/hjb.33.6.1541-1551
UR  - https://doi.org/10.4308/hjb.33.6.1541-1551
AB  - Recently, Staphylococcus aureus and Pseudomonas aeruginosa have become multidrug-resistant (MDR) and produce many virulence factors, posing a health risk. Therefore, addressing knowledge gaps and finding solutions to the challenge caused by bacteria's increased resistance to multiple antibiotics is an urgent need. To address this risk, we aim to synthesize new nanoparticles with long-lasting, safe effects and low toxicity. Metal–organic frameworks (MOFs) are promising materials with high efficiency in inhibiting or killing MDR bacteria. In this investigation, we successfully synthesized copper-doped ZIF-8 (Cu-ZIF-8) nanocomposites using the solvothermal method. Its characteristics were studied using techniques like SEM, FTIR, EDX, and XRD. The Cu-ZIF-8-MOF exhibited high antibacterial efficacy against S. aureus (Gram-positive) and P. aeruginosa (Gram-negative), with concentrations of 12.5, 25, 50, and 100 μg/mL showing high efficiency against P. aeruginosa. In contrast, it was less effective against S. aureus. These results open up wide prospects for the application of the Cu-ZIF-8-MOF antimicrobial agent.
SN  - 1978-3019
SN  - 2086-4094
ER  -

Abstract

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