Green synthesis of copper oxide nanoparticles ST@CuONPs by Streptomyces thinghirensis: current treatment path against multidrug-resistant bacteria
6 affiliations
- 1Laboratoire de Lutte Contre les Maladies Infectieuses, Faculté de Médecine et de Pharmacie, Université Cadi Ayyad
- 2Université de Reims Champagne-Ardenne, INRAE, RIBP UMR 1488
- 3Molecular Chemistry Institute of Reims (ICMR), UMR CNRS 7312, University of Reims Champagne Ardenne
- 4Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide/CSIC/Junta de Andalucía
- 5Departamento de Biología Molecular e Ingeniería Bioquímica, Universidad Pablo de Olavide
- 6Laboratoire de Microbiologie, CHU Mohammed VI
Abstract
Background: Antibiotic resistance is currently a global concern, leading to serious complications in both clinical and non-clinical settings, and is particularly dreaded in developing countries. In the search for alternative treatments against bacterial infections, nanotechnology has opened up unexpected new horizons in the scientific world, thanks to the great variety of available nanomaterials that have potential antimicrobial activity. Some of the interest in this type of nanomaterials relies in their exceptional properties and usages in many fields. The investigation focused on the synthesis of a new copper oxide nanoparticles ST@CuONPs as a promising alternative to conventional antibiotics using Streptomyces thinghirensis (DSM 41919 T ) as a novel, bacteria-mediated synthesis pathway.
Methods: The ST@CuONPs were confirmed and characterized by UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, particle-size analyzer, scanning electron microscopy (SEM), X-ray diffraction (XRD) and transmission electron microscopy (TEM). The antimicrobial and antibiofilm activities of ST@CuONPs were evaluated against Vancomycin-resistant Enterococcus faecium VRE ATCC 700221vanA, Methicillin-resistant Staphylococcus aureus NCTC 12493 and Ceftazidime-Resistant Pseudomonas aeruginosa ATCC BAA-2108, as well as Vancomycin-sensitive Enterococcus faecalis ATCC 29212, Methicillin-sensitive Staphylococcus aureus ATCC 29213 and Ceftazidime-sensitive P. aeruginosa ATCC 27853.
Results and Discussion: TEM analysis revealed a spherical shape distribution of ST@CuONPs with an average size of ∼9.8 ± 0.27 nm. The ST@CuONPs demonstrated broad-spectrum antibacterial and antibiofilm activities to ward Multidrug-resistant bacteria. In addition, among the tested multi-drug resistant (MDR) pathogens, the highest antibacterial and antibiofilm efficacies were observed against the Gram-positive strain E. faecium VREATCC 700221vanA. The bacterial biofilm was reduced by 64% ± 0.58% at 1/4 × MIC, 83% ± 3% at 1/2 × MIC and 89% ± 1.15% at 1×MIC of ST@CuONP.
Conclusion: The synthesis of ST@CuONPs using S. thinghirensis is an eco-friendly and economical method that avoids the use of toxic reagents. The resulting nanoparticles exhibit a potential to be explored as an alternative to conventional antibiotics.
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BibTeX
@article{loqman2026green,
title = {{Green synthesis of copper oxide nanoparticles ST@CuONPs by Streptomyces thinghirensis: current treatment path against multidrug-resistant bacteria}},
author = {Amal Loqman and Qassim Esmaeel and Stéphanie Sayen and Simon Remy and Jean-Hugues Renault and Younes Smani and Souad Loqman},
journal = {Frontiers in Pharmacology},
year = {2026},
month = oct,
doi = {10.3389/fphar.2026.1904965},
url = {https://www.frontiersin.org/articles/10.3389/fphar.2026.1904965/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Green synthesis of copper oxide nanoparticles ST@CuONPs by Streptomyces thinghirensis: current treatment path against multidrug-resistant bacteria
AU - Amal Loqman
AU - Qassim Esmaeel
AU - Stéphanie Sayen
AU - Simon Remy
AU - Jean-Hugues Renault
AU - Younes Smani
AU - Souad Loqman
PY - 2026
DA - 2026/10/15
JO - Frontiers in Pharmacology
DO - 10.3389/fphar.2026.1904965
UR - https://www.frontiersin.org/articles/10.3389/fphar.2026.1904965/full
AB - Background: Antibiotic resistance is currently a global concern, leading to serious complications in both clinical and non-clinical settings, and is particularly dreaded in developing countries. In the search for alternative treatments against bacterial infections, nanotechnology has opened up unexpected new horizons in the scientific world, thanks to the great variety of available nanomaterials that have potential antimicrobial activity. Some of the interest in this type of nanomaterials relies in their exceptional properties and usages in many fields. The investigation focused on the synthesis of a new copper oxide nanoparticles ST@CuONPs as a promising alternative to conventional antibiotics using Streptomyces thinghirensis (DSM 41919 T ) as a novel, bacteria-mediated synthesis pathway. Methods: The ST@CuONPs were confirmed and characterized by UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, particle-size analyzer, scanning electron microscopy (SEM), X-ray diffraction (XRD) and transmission electron microscopy (TEM). The antimicrobial and antibiofilm activities of ST@CuONPs were evaluated against Vancomycin-resistant Enterococcus faecium VRE ATCC 700221vanA, Methicillin-resistant Staphylococcus aureus NCTC 12493 and Ceftazidime-Resistant Pseudomonas aeruginosa ATCC BAA-2108, as well as Vancomycin-sensitive Enterococcus faecalis ATCC 29212, Methicillin-sensitive Staphylococcus aureus ATCC 29213 and Ceftazidime-sensitive P. aeruginosa ATCC 27853. Results and Discussion: TEM analysis revealed a spherical shape distribution of ST@CuONPs with an average size of ∼9.8 ± 0.27 nm. The ST@CuONPs demonstrated broad-spectrum antibacterial and antibiofilm activities to ward Multidrug-resistant bacteria. In addition, among the tested multi-drug resistant (MDR) pathogens, the highest antibacterial and antibiofilm efficacies were observed against the Gram-positive strain E. faecium VREATCC 700221vanA. The bacterial biofilm was reduced by 64% ± 0.58% at 1/4 × MIC, 83% ± 3% at 1/2 × MIC and 89% ± 1.15% at 1×MIC of ST@CuONP. Conclusion: The synthesis of ST@CuONPs using S. thinghirensis is an eco-friendly and economical method that avoids the use of toxic reagents. The resulting nanoparticles exhibit a potential to be explored as an alternative to conventional antibiotics.
SN - 1663-9812
ER -