The DNA methylation landscape of prostate cancer is associated with the lipid composition of periprostatic adipose tissue
Abstract
DNA methylation, a major epigenetic event associated with prostate cancer (PCa) progression, has been suggested to be influenced by the lipid microenvironment. We aimed to analyze the link between DNA methylation in PCa tissues and the lipid composition of peri-prostatic adipose tissue (PPAT), together with markers of PCa aggressiveness. Global DNA and gene-specific methylation events were evaluated using qMSRE analyses and immunohistochemistry, in cancer tissues from 75 patients treated by prostatectomy for PCa, together with the characterization of the lipid composition of PPAT. The effects of fatty acids (FA) on DNA methylation were measured in PCa cell lines. High 5mC levels correlated with features of PCa aggressiveness. 5hmC levels were associated with cholesterol content in PPAT, and PD-L1 methylation was positively linked to linoleic acid content. Moreover, decreased 5mC level and low GSTP1 methylation were associated with high n-3 FA content in PPAT. Cell lines experiments suggested a causal relationship between FA exposure and methylation events, mediated by DNMT recruitment. We identified a link between lipid content in PPAT and methylation events in PCa tissues, supported by cell lines experiments, that suggest that FA could influence PCa aggressiveness.
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BibTeX
@article{espibastien2026methylation,
title = {{The DNA methylation landscape of prostate cancer is associated with the lipid composition of periprostatic adipose tissue}},
author = {Dorian Espi-Bastien and Nathanael Fort and Cyrille Guimaraes and Arulraj Nadaradjane and Frank Bruyere and Christophe Vandier and Pierre-Francois Cartron and Gaelle Fromont},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-65221-0},
url = {https://nantes-universite.hal.science/hal-05754181v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - The DNA methylation landscape of prostate cancer is associated with the lipid composition of periprostatic adipose tissue
AU - Dorian Espi-Bastien
AU - Nathanael Fort
AU - Cyrille Guimaraes
AU - Arulraj Nadaradjane
AU - Frank Bruyere
AU - Christophe Vandier
AU - Pierre-Francois Cartron
AU - Gaelle Fromont
PY - 2026
DA - 2026/07/31
JO - Scientific Reports
DO - 10.1038/s41598-026-65221-0
UR - https://nantes-universite.hal.science/hal-05754181v1
AB - DNA methylation, a major epigenetic event associated with prostate cancer (PCa) progression, has been suggested to be influenced by the lipid microenvironment. We aimed to analyze the link between DNA methylation in PCa tissues and the lipid composition of peri-prostatic adipose tissue (PPAT), together with markers of PCa aggressiveness. Global DNA and gene-specific methylation events were evaluated using qMSRE analyses and immunohistochemistry, in cancer tissues from 75 patients treated by prostatectomy for PCa, together with the characterization of the lipid composition of PPAT. The effects of fatty acids (FA) on DNA methylation were measured in PCa cell lines. High 5mC levels correlated with features of PCa aggressiveness. 5hmC levels were associated with cholesterol content in PPAT, and PD-L1 methylation was positively linked to linoleic acid content. Moreover, decreased 5mC level and low GSTP1 methylation were associated with high n-3 FA content in PPAT. Cell lines experiments suggested a causal relationship between FA exposure and methylation events, mediated by DNMT recruitment. We identified a link between lipid content in PPAT and methylation events in PCa tissues, supported by cell lines experiments, that suggest that FA could influence PCa aggressiveness.
SN - 2045-2322
ER -