Beyond Seizure Suppression: Caffeic Acid Phenethyl Ester and 10-Hydroxy-2-Decenoic Acid Improve Anxiety-like and Cognitive Behaviors in PTZ-Challenged Zebrafish.
7 affiliations
- 1Department of Aquaculture, Faculty of Fisheries, Fırat University, Elazig 23200, Türkiye.
- 2Department of Basic Sciences, Faculty of Fisheries, Fırat University, Elazig 23200, Türkiye.
- 3Department of Aquaculture, Faculty of Agriculture, Malatya Turgut Özal University, Malatya 44210, Türkiye.
- 4Department of Agricultural Biotechnology, Faculty of Agriculture, Erciyes University, Kayseri 38039, Türkiye.
- 5Nutral Therapy Co., Erciyes Technopark, Kayseri 38039, Türkiye.
- 6Department of Child and Adolescent Mental Health and Diseases, Children's Hospital, Faculty of Medicine, Ege University, Izmir 35100, Türkiye.
- 7Department of Animal Nutrition, Faculty of Veterinary Medicine, Fırat University, Elazig 23200, Türkiye.
Abstract
Epilepsy is associated with recurrent seizures and behavioral and cognitive disturbances, highlighting the importance of assessing behavioral outcomes alongside seizure severity. Caffeic acid phenethyl ester (CAPE) has shown neuroprotective activity, whereas evidence for the neurobehavioral effects of 10-hydroxy-2-decenoic acid (10-HDA) remains limited. This study investigated their individual and combined dietary effects on seizure-like behavior and behavioral test performance in adult zebrafish (Danio rerio) challenged with pentylenetetrazol (PTZ). Fish received experimental diets for 8 weeks before exposure to 15 mM PTZ. A dietary valproic acid group was included as a comparator. Outcomes included behavioral seizure scores, the area under the seizure score-time curve (AUC), and performance in the novel tank diving and Y-maze tests. Compared with PTZ alone, CAPE, 10-HDA, and their combination reduced seizure scores and AUC values and increased upper-zone and novel-arm exploration (p < 0.05). The combined group spent more time in the novel arm than either single-compound group (p < 0.05), although its advantages varied across endpoints and pharmacological synergy was not established. Overall, dietary CAPE and 10-HDA were associated with lower behavioral seizure scores and improved test performance following acute PTZ challenge. Further studies are needed to distinguish these responses from independent effects on baseline behavior and the influence of seizure severity and motor function.
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BibTeX
@article{yonar2026beyond,
title = {{Beyond Seizure Suppression: Caffeic Acid Phenethyl Ester and 10-Hydroxy-2-Decenoic Acid Improve Anxiety-like and Cognitive Behaviors in PTZ-Challenged Zebrafish.}},
author = {Muhammet Enis Yonar and Mücahit Eroğlu and Ünal İspir and Sibel Silici and Seda Özcan and Feyza Karahüseyinoğlu and Tuba Çakmak Duran and Cemal Orhan and Serpil Mişe Yonar},
journal = {Veterinary sciences},
year = {2026},
month = oct,
doi = {10.3390/vetsci13101050},
url = {https://europepmc.org/article/MED/42944146},
note = {CC BY},
}
RIS
TY - JOUR
TI - Beyond Seizure Suppression: Caffeic Acid Phenethyl Ester and 10-Hydroxy-2-Decenoic Acid Improve Anxiety-like and Cognitive Behaviors in PTZ-Challenged Zebrafish.
AU - Muhammet Enis Yonar
AU - Mücahit Eroğlu
AU - Ünal İspir
AU - Sibel Silici
AU - Seda Özcan
AU - Feyza Karahüseyinoğlu
AU - Tuba Çakmak Duran
AU - Cemal Orhan
AU - Serpil Mişe Yonar
PY - 2026
DA - 2026/10/07
JO - Veterinary sciences
DO - 10.3390/vetsci13101050
UR - https://europepmc.org/article/MED/42944146
AB - Epilepsy is associated with recurrent seizures and behavioral and cognitive disturbances, highlighting the importance of assessing behavioral outcomes alongside seizure severity. Caffeic acid phenethyl ester (CAPE) has shown neuroprotective activity, whereas evidence for the neurobehavioral effects of 10-hydroxy-2-decenoic acid (10-HDA) remains limited. This study investigated their individual and combined dietary effects on seizure-like behavior and behavioral test performance in adult zebrafish (Danio rerio) challenged with pentylenetetrazol (PTZ). Fish received experimental diets for 8 weeks before exposure to 15 mM PTZ. A dietary valproic acid group was included as a comparator. Outcomes included behavioral seizure scores, the area under the seizure score-time curve (AUC), and performance in the novel tank diving and Y-maze tests. Compared with PTZ alone, CAPE, 10-HDA, and their combination reduced seizure scores and AUC values and increased upper-zone and novel-arm exploration (p < 0.05). The combined group spent more time in the novel arm than either single-compound group (p < 0.05), although its advantages varied across endpoints and pharmacological synergy was not established. Overall, dietary CAPE and 10-HDA were associated with lower behavioral seizure scores and improved test performance following acute PTZ challenge. Further studies are needed to distinguish these responses from independent effects on baseline behavior and the influence of seizure severity and motor function.
SN - 2306-7381
ER -