Capsaicin Modulates DNA Damage Related Gene Expressions in Cisplatin-Induced Ototoxicity.
- 1Department of Basic Oncology Dokuz Eylül University Institute of Oncology Izmir Turkey.
- 2Department of Otorhinolaryngology Dokuz Eylül University Faculty of Medicine İzmir Turkey.
Abstract
Objectives
Cisplatin (CDDP) is a potent anticancer agent with significant side effects, including ototoxicity. Capsaicin (CAPS), the active compound in chili peppers, has shown protective effects against CDDP-induced toxicities. This study aimed to investigate the role of capsaicin in CDDP-induced ototoxicity via DNA damage and apoptotic cell death pathways in House Ear Institute Organ of Corti 1 (HEI-OC1) cells and to evaluate its effects on neuroblastoma (KELLY) cells under the same conditions.Methods
HEI-OC1 and KELLY neuroblastoma cells were treated with CDDP (100 μM), CAPS (5 μM), and CAPS+CDDP combinations for 24 h. Cell viability was assessed using the WST-1 assay, and apoptosis was evaluated by Annexin V/PI flow cytometry. DNA damage-related gene expressions were analyzed in HEI-OC1 cells using a real-time PCR array. CAPS alone did not alter the viability of HEI-OC1 or KELLY cells. CDDP significantly reduced the viability of both.Results
CAPS + CDDP combination significantly decreased KELLY cell viability, while only slightly affecting HEI-OC1 cells. Both CDDP alone and CAPS + CDDP induced similar levels of apoptosis in HEI-OC1 cells. In KELLY cells, the combination induced greater apoptosis than CDDP alone. CDDP treatment upregulated DNA damage-related genes and downregulated DNA repair genes in HEI-OC1 cells. CAPS + CDDP treatment altered expression profiles, suggesting a role in DNA repair modulation.Conclusion
Capsaicin may serve as a moderate protective agent against cisplatin-induced ototoxicity without compromising cisplatin's cytotoxic effects on neuroblastoma cells. Changes in DNA repair-related gene expression support capsaicin's involvement in modulating the cellular response to cisplatin.
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BibTeX
@article{altun2026capsaicin,
title = {{Capsaicin Modulates DNA Damage Related Gene Expressions in Cisplatin-Induced Ototoxicity.}},
author = {Yavuz Kerem Altun and Yüksel Olgun and Ayça Pamukoğlu Kaynar and Safiye Aktaş and Zekiye Altun},
journal = {Laryngoscope investigative otolaryngology},
year = {2026},
month = oct,
doi = {10.1002/lio2.70598},
url = {https://europepmc.org/article/MED/42910052},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Capsaicin Modulates DNA Damage Related Gene Expressions in Cisplatin-Induced Ototoxicity.
AU - Yavuz Kerem Altun
AU - Yüksel Olgun
AU - Ayça Pamukoğlu Kaynar
AU - Safiye Aktaş
AU - Zekiye Altun
PY - 2026
DA - 2026/10/21
JO - Laryngoscope investigative otolaryngology
DO - 10.1002/lio2.70598
UR - https://europepmc.org/article/MED/42910052
AB - Objectives Cisplatin (CDDP) is a potent anticancer agent with significant side effects, including ototoxicity. Capsaicin (CAPS), the active compound in chili peppers, has shown protective effects against CDDP-induced toxicities. This study aimed to investigate the role of capsaicin in CDDP-induced ototoxicity via DNA damage and apoptotic cell death pathways in House Ear Institute Organ of Corti 1 (HEI-OC1) cells and to evaluate its effects on neuroblastoma (KELLY) cells under the same conditions.Methods HEI-OC1 and KELLY neuroblastoma cells were treated with CDDP (100 μM), CAPS (5 μM), and CAPS+CDDP combinations for 24 h. Cell viability was assessed using the WST-1 assay, and apoptosis was evaluated by Annexin V/PI flow cytometry. DNA damage-related gene expressions were analyzed in HEI-OC1 cells using a real-time PCR array. CAPS alone did not alter the viability of HEI-OC1 or KELLY cells. CDDP significantly reduced the viability of both.Results CAPS + CDDP combination significantly decreased KELLY cell viability, while only slightly affecting HEI-OC1 cells. Both CDDP alone and CAPS + CDDP induced similar levels of apoptosis in HEI-OC1 cells. In KELLY cells, the combination induced greater apoptosis than CDDP alone. CDDP treatment upregulated DNA damage-related genes and downregulated DNA repair genes in HEI-OC1 cells. CAPS + CDDP treatment altered expression profiles, suggesting a role in DNA repair modulation.Conclusion Capsaicin may serve as a moderate protective agent against cisplatin-induced ototoxicity without compromising cisplatin's cytotoxic effects on neuroblastoma cells. Changes in DNA repair-related gene expression support capsaicin's involvement in modulating the cellular response to cisplatin.
SN - 2378-8038
ER -