Skip to content
DraconiticResearch
Search Sources API
206,698 studies31 sourcesUpdated 31 min ago
Peer-reviewedCC BYScience (New York, N.Y.)

Mechanism of membrane perforation in rotavirus cell entry

  • Marilina de Sautu1,2
  • Conny Leistner3
  • Tomas Kirchhausen4,5,6
  • Simon Jenni1
  • Stephen C. Harrison1,2,7
7 affiliations
  1. 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School; Boston, Massachusetts, United States of America
  2. 2Laboratory of Molecular Medicine, Boston Children’s Hospital; Boston, Massachusetts, United States of America
  3. 3Harvard cryo-EM Center for Structural Biology, Harvard Medical School; Boston, Massachusetts, United States of America
  4. 4Department of Cell Biology, Harvard Medical School, Boston, Massachusetts; United States of America
  5. 5Department of Pediatrics, Harvard Medical School, Boston, Massachusetts; United States of America
  6. 6Program in Cellular and Molecular Medicine Boston Children’s Hospital; Boston, Massachusetts, United States of America
  7. 7Howard Hughes Medical Institute, Harvard Medical School; Boston, Massachusetts, United States of America
Read full textDOICite

Abstract

Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid particle that does not uncoat further. Instead, it extrudes capped viral mRNA by virtue of polymerase and capping activities within it. As described here, we used cryogenic electron tomography to visualize the full course of rhesus rotavirus entry, from cell attachment and virion uptake to release of the subviral particle. The cryo-tomograms and subtomogram averaging of classified subparticles link high-resolution structures of the virion and its components with time series from live-cell fluorescence microscopy. We outline the mechanism of each step in the entry process, including the membrane perforation step that transfers a subviral particle into the cytosol.

Related

  1. Secretory Phospholipase A2–Albumin–Membrane Interactions: A Proposed Mechanism of Internal Cell and Tissue Injury in Systemic Inflammation
    Francis H. C. Tsao, Keith C. Meyer
    BiomoleculesPeer-reviewedCC BY 4.0Epidemiology & public healthCrossref
  2. Quantification of Calcium Entry at the T-Tubules and Surface Membrane in Rat Ventricular Myocytes
    F. Brette, L. Sallé, C.H. Orchard
    Biophysical JournalPeer-reviewedLicence not stated85 citedCardiovascular medicineHAL
  3. Cellular and Tissue Mechanisms of Laser-Assisted Regeneration of Tympanic Membrane Perforation
    Ekaterina Valerievna Blinova, Igor Vyacheslavovich Lunyov, Andrey Igorevich Osipyants +10
    International journal of molecular sciencesPeer-reviewedCC BYFull textMedical imaging & radiologyEurope PMCCrossref
  4. Recurrent Corneal Erosion Syndrome in Epithelial Basement Membrane Dystrophy: Current Treatments and Mechanism-Guided Therapeutic Opportunities
    Sujin Kang, Alfonso L. Sabater, Sotiria Palioura +1
    CellsPeer-reviewedCC BY 4.0Full textMedical imaging & radiologyCrossrefEurope PMC
  5. Calendar-Period Variation in the Association Between Documented Complete Rotavirus Vaccination and Laboratory-Confirmed Gastroenteritis: An 11-Year Test-Negative Study
    Yunus Nas, Basri Çakıroğlu
    Pediatric reportsPeer-reviewedCC BYFull textGeneral medicineEurope PMC

Cite

BibTeXRIS
BibTeX
@article{sautu2026mechanism,
  title = {{Mechanism of membrane perforation in rotavirus cell entry}},
  author = {Marilina de Sautu and Conny Leistner and Tomas Kirchhausen and Simon Jenni and Stephen C. Harrison},
  journal = {Science (New York, N.Y.)},
  year = {2026},
  month = sep,
  doi = {10.1126/science.aeg4851},
  url = {https://doi.org/10.1126/science.aeg4851},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Mechanism of membrane perforation in rotavirus cell entry
AU  - Marilina de Sautu
AU  - Conny Leistner
AU  - Tomas Kirchhausen
AU  - Simon Jenni
AU  - Stephen C. Harrison
PY  - 2026
DA  - 2026/09/10
JO  - Science (New York, N.Y.)
DO  - 10.1126/science.aeg4851
UR  - https://doi.org/10.1126/science.aeg4851
AB  - Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid particle that does not uncoat further. Instead, it extrudes capped viral mRNA by virtue of polymerase and capping activities within it. As described here, we used cryogenic electron tomography to visualize the full course of rhesus rotavirus entry, from cell attachment and virion uptake to release of the subviral particle. The cryo-tomograms and subtomogram averaging of classified subparticles link high-resolution structures of the virion and its components with time series from live-cell fluorescence microscopy. We outline the mechanism of each step in the entry process, including the membrane perforation step that transfers a subviral particle into the cytosol.
SN  - 0036-8075
SN  - 1095-9203
ER  -