Skip to content
DraconiticResearch
Search Sources API
181,713 studies31 sourcesUpdated 54 min ago
Peer-reviewedCC BY 4.0Polymers

Interleukin Expression and Activation of Inflammatory/Apoptosis Pathways on Human Gingival Fibroblasts Exposed to Light-Cured Dental Composites

  • Edoardo Sorrentino1
  • Camillo D’Arcangelo2
  • Antonella Mazzone1
  • Luigia Fonticoli1
  • Alessio Frisone1
  • Ludovica Coppola2
  • Emanuela Mazzon1
  • Oriana Trubiani1
  • Jacopo Pizzicannella3
  • Guya Diletta Marconi1
  • Francesca Diomede1
  • Francesco De Angelis2
  1. 1Department of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
  2. 2Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
  3. 3Department of Neuroscience, Imaging and Clinical Sciences, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
Read full textPDFDOICite

Abstract

This in vitro study aimed to analyze the activation of the NFκB p65/MyD88/NALP3 inflammatory pathway and the expression of the BCL-2/BAX/Caspase-3 apoptosis pathway on human gingival fibroblasts (hGFs) after 24 h and 1-week exposure to the eluates of three light-cured commercial composites (GR: GrandioSO, VOCO; BF: Enamel Plus HRi Biofunction, Micerium; FU: Filtek universal restorative, 3M) and the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β). A total of 50 light-cured disk-shaped specimens for each composite group (GR, BF and FU) were produced. The tested materials differed in resin matrix composition; notably, GR contained Bis-GMA, whereas BF and FU contained urethane-based dimethacrylates. The hGFs were exposed for 24 h and 1 week to eluates of each group. Pathway activation was evaluated by cell metabolic activity, immunofluorescence analysis and real-time PCR. Inflammatory effects were assessed in hGF culture by measuring proinflammatory cytokine production (ELISA). GR, BF and FU showed a reduction in metabolic activity in a time-dependent manner. GR showed a significant decrease in terms of cell viability after 1 week of exposure and a greater expression of proapoptotic markers than BF and FU. High levels of proinflammatory cytokines were measured after 1 week of culture for GR. The inflammatory pathway was activated in a time-dependent manner. Pathway activation was material-dependent; among the tested composites, GR was found to be more cytotoxic than BF and FU. Despite the limitations of an in vitro study, these findings suggest that dental composite eluates cause time- and material-dependent cytotoxicity, inflammation, and apoptosis in hGFs. Exposure significantly impairs cellular metabolic activity from 24 h up to 1 week. Among the evaluated materials, the GR composite triggered a markedly severe inflammatory and apoptotic effect compared to BF or FU. Ultimately, these findings underscore the potential clinical importance of selecting materials with optimal biocompatibility to minimize adverse tissue reactions, although their direct translation into clinical practice would require further research.

Related

  1. Evaluation of Interleukin-8 (IL-8) Expression in Human Aortic Endothelial Cells (HAECs) and Antioxidant Activity of Isolated Compounds from the Endemic Hawaiian Plant Pipturus albidus (Māmaki)
    Pornphimon Meesakul, Kyung Ah Kim, Yaru Si +6
    Molecules (Basel, Switzerland)Peer-reviewedCC BYFull textGeneral chemistryEurope PMC
  2. Mechanism-driven nano-therapeutic platform integrating plant-derived compounds and membrane-active peptides reprograms inflammatory, cell cycle arrest, cGAS–STING, and apoptotic gene expression to quench colorectal cancer
    Doaa Ibrahim, Safaa I. Khater, Ahmed Abdelfattah-Hassan +9
    Frontiers in PharmacologyPeer-reviewedCC BY 4.0Full textMaterials & polymersCrossref
  3. Radiolabeling with fluorine-18 of a protein, interleukin-1 receptor antagonist
    C. Prenant, C. Cawthorne, M. Fairclough +2
    Applied Radiation and IsotopesPeer-reviewedLicence not stated9 citedInorganic & organometallic chemistryHAL
  4. Screening colorectal tumor-associated fibroblasts using dual-channel THz biosensor
    Hongji Xu, Ruiqi Gu, Arthur Georg Serville Torvund +5
    iSciencePeer-reviewedCC BY 4.0Full textMaterials & polymersCell PressCrossrefPMCEurope PMC
  5. Cerium Dioxide Nanoparticles Modulate Ribosomal DNA Transcriptional Activity in Cultured Human Skin Fibroblasts
    Natalia N. Veiko, Elizaveta S. Ershova, Madina M. Sozarukova +9
    International journal of molecular sciencesPeer-reviewedCC BYFull textMaterials & polymersEurope PMCCrossref

Cite

BibTeXRIS
BibTeX
@article{sorrentino2026interleukin,
  title = {{Interleukin Expression and Activation of Inflammatory/Apoptosis Pathways on Human Gingival Fibroblasts Exposed to Light-Cured Dental Composites}},
  author = {Edoardo Sorrentino and Camillo D’Arcangelo and Antonella Mazzone and Luigia Fonticoli and Alessio Frisone and Ludovica Coppola and Emanuela Mazzon and Oriana Trubiani and Jacopo Pizzicannella and Guya Diletta Marconi and Francesca Diomede and Francesco De Angelis},
  journal = {Polymers},
  year = {2026},
  month = oct,
  doi = {10.3390/polym18202484},
  url = {https://www.mdpi.com/2073-4360/18/20/2484},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Interleukin Expression and Activation of Inflammatory/Apoptosis Pathways on Human Gingival Fibroblasts Exposed to Light-Cured Dental Composites
AU  - Edoardo Sorrentino
AU  - Camillo D’Arcangelo
AU  - Antonella Mazzone
AU  - Luigia Fonticoli
AU  - Alessio Frisone
AU  - Ludovica Coppola
AU  - Emanuela Mazzon
AU  - Oriana Trubiani
AU  - Jacopo Pizzicannella
AU  - Guya Diletta Marconi
AU  - Francesca Diomede
AU  - Francesco De Angelis
PY  - 2026
DA  - 2026/10/11
JO  - Polymers
DO  - 10.3390/polym18202484
UR  - https://www.mdpi.com/2073-4360/18/20/2484
AB  - This in vitro study aimed to analyze the activation of the NFκB p65/MyD88/NALP3 inflammatory pathway and the expression of the BCL-2/BAX/Caspase-3 apoptosis pathway on human gingival fibroblasts (hGFs) after 24 h and 1-week exposure to the eluates of three light-cured commercial composites (GR: GrandioSO, VOCO; BF: Enamel Plus HRi Biofunction, Micerium; FU: Filtek universal restorative, 3M) and the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β). A total of 50 light-cured disk-shaped specimens for each composite group (GR, BF and FU) were produced. The tested materials differed in resin matrix composition; notably, GR contained Bis-GMA, whereas BF and FU contained urethane-based dimethacrylates. The hGFs were exposed for 24 h and 1 week to eluates of each group. Pathway activation was evaluated by cell metabolic activity, immunofluorescence analysis and real-time PCR. Inflammatory effects were assessed in hGF culture by measuring proinflammatory cytokine production (ELISA). GR, BF and FU showed a reduction in metabolic activity in a time-dependent manner. GR showed a significant decrease in terms of cell viability after 1 week of exposure and a greater expression of proapoptotic markers than BF and FU. High levels of proinflammatory cytokines were measured after 1 week of culture for GR. The inflammatory pathway was activated in a time-dependent manner. Pathway activation was material-dependent; among the tested composites, GR was found to be more cytotoxic than BF and FU. Despite the limitations of an in vitro study, these findings suggest that dental composite eluates cause time- and material-dependent cytotoxicity, inflammation, and apoptosis in hGFs. Exposure significantly impairs cellular metabolic activity from 24 h up to 1 week. Among the evaluated materials, the GR composite triggered a markedly severe inflammatory and apoptotic effect compared to BF or FU. Ultimately, these findings underscore the potential clinical importance of selecting materials with optimal biocompatibility to minimize adverse tissue reactions, although their direct translation into clinical practice would require further research.
SN  - 2073-4360
ER  -