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Peer-reviewedCC BY 4.0STAR Protocols

Protocol for visualizing microtubules in transzonal projections of mouse ovarian follicles

  • Takao Tsurumaki1
  • Akihiro Aikawa1
  • Masamitsu Sato1,2
  • Mika Toya1,3
  1. 1Laboratory of Cytoskeletal Logistics, Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan
  2. 2Institute for Medical-Oriented Structural Biology, Waseda University, Shinjuku-ku, Tokyo, Japan
  3. 3Laboratory for Histogenetic Dynamics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan
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Abstract

Visualizing microtubules within tissues remains technically challenging, limiting our understanding of their organization and function in vivo. Here, we present a protocol for visualizing microtubules in transzonal projections (TZPs), cellular projections that connect ovarian somatic cells and oocytes within mouse ovarian follicles. We describe steps and conditions for ovarian fixation, cryosection preparation, and immunostaining to preserve fine microtubule structures. We then detail procedures for super-resolution imaging to visualize fine microtubules within TZPs.

For complete details on the use and execution of this protocol, please refer to Aikawa et al.1

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Cite

BibTeXRIS
BibTeX
@article{tsurumaki2026protocol,
  title = {{Protocol for visualizing microtubules in transzonal projections of mouse ovarian follicles}},
  author = {Takao Tsurumaki and Akihiro Aikawa and Masamitsu Sato and Mika Toya},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104924},
  url = {https://doi.org/10.1016/j.xpro.2026.104924},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for visualizing microtubules in transzonal projections of mouse ovarian follicles
AU  - Takao Tsurumaki
AU  - Akihiro Aikawa
AU  - Masamitsu Sato
AU  - Mika Toya
PY  - 2026
DA  - 2026/10/28
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104924
UR  - https://doi.org/10.1016/j.xpro.2026.104924
AB  - Visualizing microtubules within tissues remains technically challenging, limiting our understanding of their organization and function in vivo. Here, we present a protocol for visualizing microtubules in transzonal projections (TZPs), cellular projections that connect ovarian somatic cells and oocytes within mouse ovarian follicles. We describe steps and conditions for ovarian fixation, cryosection preparation, and immunostaining to preserve fine microtubule structures. We then detail procedures for super-resolution imaging to visualize fine microtubules within TZPs.  For complete details on the use and execution of this protocol, please refer to Aikawa et al.1
SN  - 2666-1667
ER  -