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Peer-reviewedCC BY 4.0Nature Methods

Continuous mass photometry by single-molecule trapping

  • Dan Loewenthal1,2
  • Roi Asor1,2
  • Stephen Thorpe1,2
  • Jack S. Peters1,2
  • Konstantin C. Zouboulis1,2
  • Merve Kaplan1,2,3
  • Jan Christoph Thiele1,2
  • Jack Bardzil1,2
  • Seham Helmi1,2
  • Sean A. Burnap1,2,3
  • Tiong Kit Tan4,5
  • Weston B. Struwe2,3
2 more authors
  • Justin L. P. Benesch1,2
  • Philipp Kukura1,2
5 affiliations
  1. 1Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK
  2. 2Biochemistry Building, Kavli Institute for Nanoscience Discovery, Oxford, UK
  3. 3Department of Biochemistry, University of Oxford, Oxford, UK
  4. 4MRC Translational Immune Discovery Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
  5. 5CAMS Oxford Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, University of Oxford, Oxford, UK
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Abstract

The dynamic choreography of biomolecular interactions underpins the processes of life, but its direct observation remains challenging. Here we introduce continuous mass photometry, enabling hour-long, mass-resolved observation of individual biomolecules tethered to supported lipid bilayers, their complexes and interactions, with up to sub-kilodalton mass precision and millisecond temporal resolution. Our approach represents a quantitative time-resolved single-molecule measurement modality for studying complex biomolecular mechanisms in action.

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Cite

BibTeXRIS
BibTeX
@article{loewenthal2026continuous,
  title = {{Continuous mass photometry by single-molecule trapping}},
  author = {Dan Loewenthal and Roi Asor and Stephen Thorpe and Jack S. Peters and Konstantin C. Zouboulis and Merve Kaplan and Jan Christoph Thiele and Jack Bardzil and Seham Helmi and Sean A. Burnap and Tiong Kit Tan and Weston B. Struwe and Justin L. P. Benesch and Philipp Kukura},
  journal = {Nature Methods},
  year = {2026},
  month = oct,
  doi = {10.1038/s41592-026-03252-1},
  url = {https://doi.org/10.1038/s41592-026-03252-1},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Continuous mass photometry by single-molecule trapping
AU  - Dan Loewenthal
AU  - Roi Asor
AU  - Stephen Thorpe
AU  - Jack S. Peters
AU  - Konstantin C. Zouboulis
AU  - Merve Kaplan
AU  - Jan Christoph Thiele
AU  - Jack Bardzil
AU  - Seham Helmi
AU  - Sean A. Burnap
AU  - Tiong Kit Tan
AU  - Weston B. Struwe
AU  - Justin L. P. Benesch
AU  - Philipp Kukura
PY  - 2026
DA  - 2026/10/26
JO  - Nature Methods
DO  - 10.1038/s41592-026-03252-1
UR  - https://doi.org/10.1038/s41592-026-03252-1
AB  - The dynamic choreography of biomolecular interactions underpins the processes of life, but its direct observation remains challenging. Here we introduce continuous mass photometry, enabling hour-long, mass-resolved observation of individual biomolecules tethered to supported lipid bilayers, their complexes and interactions, with up to sub-kilodalton mass precision and millisecond temporal resolution. Our approach represents a quantitative time-resolved single-molecule measurement modality for studying complex biomolecular mechanisms in action.
SN  - 1548-7091
SN  - 1548-7105
ER  -