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PreprintCC BYbioRxiv

High-performance xenograft read-sorting for DNA methylation analysis

  • S. Altendorfer
  • J. Zentgraf
  • J. E. Schmitz
  • C. Plass
  • C. Gerhauser
  • S. Rahmann1
  1. 1Saarland University
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Abstract

Xenograft sorting tools separate sequenced DNA reads by species. We have enhanced the computational method and tool Xengsort to enable efficient epigenetic profiling of patient-derived xenograft models; it now supports cytosine-converted whole genome or reduced-representation data. Benchmarking Xengsort against several established methods (ICRG, bamcmp, XenofilteR) revealed consistently high accuracy across tools, but Xengsort outperformed the other tools in computational efficiency due to its alignment-free design.

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Cite

BibTeXRIS
BibTeX
@misc{altendorfer2026highperformance,
  title = {{High-performance xenograft read-sorting for DNA methylation analysis}},
  author = {S. Altendorfer and J. Zentgraf and J. E. Schmitz and C. Plass and C. Gerhauser and S. Rahmann},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.06.756952},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.06.756952v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - High-performance xenograft read-sorting for DNA methylation analysis
AU  - S. Altendorfer
AU  - J. Zentgraf
AU  - J. E. Schmitz
AU  - C. Plass
AU  - C. Gerhauser
AU  - S. Rahmann
PY  - 2026
DA  - 2026/10/06
PB  - bioRxiv
DO  - 10.64898/2026.10.06.756952
UR  - https://www.biorxiv.org/content/10.64898/2026.10.06.756952v1
AB  - Xenograft sorting tools separate sequenced DNA reads by species. We have enhanced the computational method and tool Xengsort to enable efficient epigenetic profiling of patient-derived xenograft models; it now supports cytosine-converted whole genome or reduced-representation data. Benchmarking Xengsort against several established methods (ICRG, bamcmp, XenofilteR) revealed consistently high accuracy across tools, but Xengsort outperformed the other tools in computational efficiency due to its alignment-free design.
ER  -