Transcriptomic and Epigenetic Reprogramming in Solanum tuberosum L. Micropropagation: A Systematic Review of Elicitor-Driven Acclimatization and Scalable Biofactories
- 1Grupo de Investigación Agricultura Sostenible (GIAS), Carrera de Agropecuaria, Facultad de Industrias Agropecuarias y Ciencias Ambientales, Universidad Politécnica Estatal del Carchi, Tulcán 040102, Ecuador
- 2Carrera de Alimentos, Facultad de Industrias Agropecuarias y Ciencias Ambientales, Universidad Politécnica Estatal Del Carchi, Tulcán 040102, Ecuador
- 3Grupo de Investigación en Fruticultura y Técnicas de Producción, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Universidad Miguel Hernández, Carretera de Beniel, Km 3.2, 03312 Orihuela, Spain
Abstract
Solanum tuberosum L. relies heavily on in vitro propagation for pathogen-free seed tuber production; however, the transition to ex vitro environments causes severe plantlet mortality due to transplant shock. Exogenous elicitors have emerged as pivotal preconditioning tools, but their underlying molecular mechanisms remain fragmented. This systematic review aims to elucidate the translational architecture of elicitor-driven preconditioning in Solanum tuberosum, systematically organized across a tripartite continuum—(1) epigenetic chromatin plasticity, (2) transcriptomic defense reprogramming, and (3) scalable biofactory platforms (temporary immersion bioreactors and sensor-actuated aeroponics)—required to validate ex vitro physiological competence. Adhering to PRISMA 2020 guidelines for systematic reviews, we conducted a structured search across Scopus, Web of Science, and ScienceDirect (January 2010 to August 2026). Inclusion criteria targeted original research evaluating transcriptomic or epigenetic responses to in vitro elicitation in S. tuberosum. Following quality appraisal, data from 58 high-quality studies were synthesized using a critical narrative approach. The evidence is clearly stratified into our three core layers. (1) Transcriptomic Reprogramming: Exogenous elicitors systematically reactivate dormant defense networks prior to ex vitro transfer, converging strictly on salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) signaling pathways alongside reactive oxygen species (ROS) detoxification. (2) Epigenetic Plasticity: Concurrently, morphological adaptation is heavily modulated by reversible chromatin remodeling; specifically, transient DNA hypomethylation and histone acetylation ensure in vitro cellular totipotency and stress memory. (3) Translational Scalability: Automated bioreactors and aeroponic platforms provide the essential physical microenvironment to validate these molecular outcomes and reverse vitrotype disorders at commercial scales. Elicitors drive an integrated tripartite preconditioning continuum that mitigates transplant shock and enables scalable hardening. We delineate a comprehensive translational framework to the transition from empirical micropropagation to targeted, genomics-informed biofactory platforms.
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BibTeX
@article{sarchi2026transcriptomic,
title = {{Transcriptomic and Epigenetic Reprogramming in Solanum tuberosum L. Micropropagation: A Systematic Review of Elicitor-Driven Acclimatization and Scalable Biofactories}},
author = {Guillermo Alexander Jácome Sarchi and Stalin Aldair De La Cruz Sarchi and Jorge Ivan Mina Ortega and Francisca Hernández and Rafael Todos Santos Martínez},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209288},
url = {https://www.mdpi.com/1422-0067/27/20/9288},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Transcriptomic and Epigenetic Reprogramming in Solanum tuberosum L. Micropropagation: A Systematic Review of Elicitor-Driven Acclimatization and Scalable Biofactories
AU - Guillermo Alexander Jácome Sarchi
AU - Stalin Aldair De La Cruz Sarchi
AU - Jorge Ivan Mina Ortega
AU - Francisca Hernández
AU - Rafael Todos Santos Martínez
PY - 2026
DA - 2026/10/19
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209288
UR - https://www.mdpi.com/1422-0067/27/20/9288
AB - Solanum tuberosum L. relies heavily on in vitro propagation for pathogen-free seed tuber production; however, the transition to ex vitro environments causes severe plantlet mortality due to transplant shock. Exogenous elicitors have emerged as pivotal preconditioning tools, but their underlying molecular mechanisms remain fragmented. This systematic review aims to elucidate the translational architecture of elicitor-driven preconditioning in Solanum tuberosum, systematically organized across a tripartite continuum—(1) epigenetic chromatin plasticity, (2) transcriptomic defense reprogramming, and (3) scalable biofactory platforms (temporary immersion bioreactors and sensor-actuated aeroponics)—required to validate ex vitro physiological competence. Adhering to PRISMA 2020 guidelines for systematic reviews, we conducted a structured search across Scopus, Web of Science, and ScienceDirect (January 2010 to August 2026). Inclusion criteria targeted original research evaluating transcriptomic or epigenetic responses to in vitro elicitation in S. tuberosum. Following quality appraisal, data from 58 high-quality studies were synthesized using a critical narrative approach. The evidence is clearly stratified into our three core layers. (1) Transcriptomic Reprogramming: Exogenous elicitors systematically reactivate dormant defense networks prior to ex vitro transfer, converging strictly on salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) signaling pathways alongside reactive oxygen species (ROS) detoxification. (2) Epigenetic Plasticity: Concurrently, morphological adaptation is heavily modulated by reversible chromatin remodeling; specifically, transient DNA hypomethylation and histone acetylation ensure in vitro cellular totipotency and stress memory. (3) Translational Scalability: Automated bioreactors and aeroponic platforms provide the essential physical microenvironment to validate these molecular outcomes and reverse vitrotype disorders at commercial scales. Elicitors drive an integrated tripartite preconditioning continuum that mitigates transplant shock and enables scalable hardening. We delineate a comprehensive translational framework to the transition from empirical micropropagation to targeted, genomics-informed biofactory platforms.
SN - 1422-0067
ER -