Skip to content
DraconiticResearch
Search Sources API
186,625 studies31 sourcesUpdated 15 min ago
PreprintCC BY-NC-NDChemRxiv

Classical Nucleation Induced Intrafibrillar Mineralization of Collagen

  • Shiwen Cui1,2
  • Shasha Yao3,4
  • Keyan Liu1,2
  • Chengkun Xing1,2
  • Mingxing Liu1,2
  • Haiqin Du1,2
  • Pilan Zhang1,2
  • Zhao Jiang5,6
  • Jiawei Li1,2
  • Guangrong Zhou1,2
  • Man Zhang1,2
  • Xiaoyue Hu1,2
1 more authors
  • Yifei Xu1,2
6 affiliations
  1. 1Department of Macromolecular Science
  2. 2Fudan University
  3. 3Department of Orthopaedic Surgery
  4. 4Sir Run Run Shaw Hospital School of Medicine, Zhejiang University
  5. 5Department of Chemistry
  6. 6Durham University
Open at sourcePDFDOICite

Abstract

Collagen mineralization is fundamental to bone formation, and this process is widely believed to proceed via an amorphous calcium phosphate (ACP)-mediated non-classical nucleation pathway. Here we investigated this paradigm using cryogenic electron tomography. The results reveal that the intrafibrillar mineralization of collagen does not rely on the infiltration of ACP, but occurs through a classical heterogeneous nucleation mechanism, which can be accelerated both in vitro and in vivo by increasing the density of the collagen template. The finding not only updates our understanding of collagen mineralization mechanisms, but also provides critical insights for the design of bone regeneration materials.

Related

  1. Hemp Seed-Derived Vesicle-like Nanoparticles Modulate Dermal Collagen Homeostasis and Attenuate UVB-Induced Photoaging-Related Responses
    Hannah S. Park, Sehyun Shin
    Molecules (Basel, Switzerland)Peer-reviewedCC BYFull textGeneral chemistryEurope PMC
  2. Substrate-induced crystallisation in polymers: Exceptional impact of prefreezing versus heterogeneous nucleation on kinetics and morphology
    Marthinus van Niekerk, Muhammad Tariq, Oleksandr Dolynchuk
    arXivPreprintCC BY 4.0Full textPhysical chemistryCondensed matterarXiv
  3. A Collagen-Derived Peptide Fraction CCAP1 from Colla Corii Asini Attenuates UVB-Induced Skin Photoaging via an AMPK-Dependent Nrf2/NF-κB Axis
    Fen Du, Zhuang Miao, Wanxiu Cao +10
    MoleculesPeer-reviewedCC BY 4.0General chemistryCrossref
  4. Active control of crystal nucleation and growth using operando X-ray imaging
    Eslam M. Elbakry, Emma A. Pappas, Joshua C. Gibson +1
    ChemRxivPreprintLicence not statedGeneral chemistryChemRxiv
  5. How Metal-to-Linker Ratio and Concentration Govern the Nucleation Mechanism of MOF-5-inspired Crystals: Insights from Multiscale Modeling
    Katherine Ardila, Alexander Pak, Diego Gomez-Gualdron
    ChemRxivPreprintLicence not statedGeneral chemistryChemRxiv

Cite

BibTeXRIS
BibTeX
@misc{cui2026classical,
  title = {{Classical Nucleation Induced Intrafibrillar Mineralization of Collagen}},
  author = {Shiwen Cui and Shasha Yao and Keyan Liu and Chengkun Xing and Mingxing Liu and Haiqin Du and Pilan Zhang and Zhao Jiang and Jiawei Li and Guangrong Zhou and Man Zhang and Xiaoyue Hu and Yifei Xu},
  howpublished = {ChemRxiv},
  year = {2026},
  month = oct,
  doi = {10.26434/chemrxiv.15010320/v1},
  url = {https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010320/v1},
  note = {CC BY-NC-ND},
}
RIS
TY  - UNPB
TI  - Classical Nucleation Induced Intrafibrillar Mineralization of Collagen
AU  - Shiwen Cui
AU  - Shasha Yao
AU  - Keyan Liu
AU  - Chengkun Xing
AU  - Mingxing Liu
AU  - Haiqin Du
AU  - Pilan Zhang
AU  - Zhao Jiang
AU  - Jiawei Li
AU  - Guangrong Zhou
AU  - Man Zhang
AU  - Xiaoyue Hu
AU  - Yifei Xu
PY  - 2026
DA  - 2026/10/12
PB  - ChemRxiv
DO  - 10.26434/chemrxiv.15010320/v1
UR  - https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010320/v1
AB  - Collagen mineralization is fundamental to bone formation, and this process is widely believed to proceed via an amorphous calcium phosphate (ACP)-mediated non-classical nucleation pathway. Here we investigated this paradigm using cryogenic electron tomography. The results reveal that the intrafibrillar mineralization of collagen does not rely on the infiltration of ACP, but occurs through a classical heterogeneous nucleation mechanism, which can be accelerated both in vitro and in vivo by increasing the density of the collagen template. The finding not only updates our understanding of collagen mineralization mechanisms, but also provides critical insights for the design of bone regeneration materials.
ER  -