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Peer-reviewedCC BY 4.0Microbiology Spectrum

Protein polymers proliferate: polymerization of phosphatases and proteases

  • Ravi R Sonani
  • Iman Chouikha
  • Thijs Bodson
  • Silvia Hidalgo-Martinez
  • Filip J R Meysman
  • Han Remaut
  • Geneviève Ball
  • Edward H Egelman
  • Romé Voulhoux
  • Mike Sleutel
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Abstract

It has been known for more than half a century that enzymes can polymerize to either activate or repress their catalytic functions. Using cryo-EM, we serendipitously found that two commonly used enzymes in molecular microbiology research, alkaline phosphatase and proteinase K, can unexpectedly form filaments. While the physiological significance of fibrillation in alkaline phosphatase and proteinase K is unknown, the atomic models of these polymers show the ability of cryo-EM to discover and determine high resolution structures of macromolecular assemblies before they have been characterized or purified.

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Cite

BibTeXRIS
BibTeX
@article{sonani2026protein,
  title = {{Protein polymers proliferate: polymerization of phosphatases and proteases}},
  author = {Ravi R Sonani and Iman Chouikha and Thijs Bodson and Silvia Hidalgo-Martinez and Filip J R Meysman and Han Remaut and Geneviève Ball and Edward H Egelman and Romé Voulhoux and Mike Sleutel},
  journal = {Microbiology Spectrum},
  year = {2026},
  month = aug,
  doi = {10.1128/spectrum.02439-26},
  url = {https://hal.science/hal-05737098v1},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Protein polymers proliferate: polymerization of phosphatases and proteases
AU  - Ravi R Sonani
AU  - Iman Chouikha
AU  - Thijs Bodson
AU  - Silvia Hidalgo-Martinez
AU  - Filip J R Meysman
AU  - Han Remaut
AU  - Geneviève Ball
AU  - Edward H Egelman
AU  - Romé Voulhoux
AU  - Mike Sleutel
PY  - 2026
DA  - 2026/08/11
JO  - Microbiology Spectrum
DO  - 10.1128/spectrum.02439-26
UR  - https://hal.science/hal-05737098v1
AB  - It has been known for more than half a century that enzymes can polymerize to either activate or repress their catalytic functions. Using cryo-EM, we serendipitously found that two commonly used enzymes in molecular microbiology research, alkaline phosphatase and proteinase K, can unexpectedly form filaments. While the physiological significance of fibrillation in alkaline phosphatase and proteinase K is unknown, the atomic models of these polymers show the ability of cryo-EM to discover and determine high resolution structures of macromolecular assemblies before they have been characterized or purified.
SN  - 2165-0497
ER  -