Enhancing Germination and Assessing the Longevity of Native Plants' Seeds with Dormancy through Different Priming Techniques
- 1Kuwait Institute for Scientific Research
Abstract
Vachellia pachyceras and Convolvulus oxyphyllus are native species of arid and semi-arid desert regions, including Kuwait. They are inherently adapted to extreme conditions characterized by high temperature, prolonged drought and limited rainfall. They contribute significantly, to restore the ecosystem by providing food and shelter for wildlife. Despite their ecological importance, seed germination in both species is constrained by dormancy, and natural recruitment of seedlings is limited by narrow and unpredictable rainfall windows, severely restricting opportunities for establishment. In this context, seed priming is an effective pre-sowing technique that enables uniform and rapid germination within the short window of favorable conditions. This study aims to identify the optimal seed-priming techniques for these two keystone native species and to assess the effect of priming on seed longevity. The seeds were scarified to remove the physical dormancy prior to priming treatments. Relative seed longevity was measured for primed seeds through artificial ageing and analyzed using probit analysis and analysis of variance. Results showed that hormonal priming [(mechanical scarification + 100 ppm gibberellic acid (GA3)] for six hours and hydropriming (mechanical scarification + soaking in water) for 8 hours (h) were effective treatments among the tested priming treatments for V. pachyceras. The estimated p50 value (i.e., number of days to lose seed viability to 50%) was 22 days for hormonal priming and 21 days for hydropriming. Mechanical scarification followed by one and half hour of hydropriming was the most effective in C. oxyphyllus (p50 = 74 days). Based on these results, the longevity of V. pachyceras and C. oxyphyllus could be considered medium. Further studies are needed to determine the effectiveness of seed priming on additional ecologically and economically important native species to achieve success in revegetation programs.
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BibTeX
@misc{suleiman2026enhancing,
title = {{Enhancing Germination and Assessing the Longevity of Native Plants' Seeds with Dormancy through Different Priming Techniques}},
author = {M. K. Suleiman and S. A. Shahid and N. R. Bhat and S. Jacob and M. T. Sivadasan and R. R. Thomas and M. A. Islam},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.30.763245},
url = {https://www.biorxiv.org/content/10.64898/2026.10.30.763245v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Enhancing Germination and Assessing the Longevity of Native Plants' Seeds with Dormancy through Different Priming Techniques
AU - M. K. Suleiman
AU - S. A. Shahid
AU - N. R. Bhat
AU - S. Jacob
AU - M. T. Sivadasan
AU - R. R. Thomas
AU - M. A. Islam
PY - 2026
DA - 2026/10/30
PB - bioRxiv
DO - 10.64898/2026.10.30.763245
UR - https://www.biorxiv.org/content/10.64898/2026.10.30.763245v1
AB - Vachellia pachyceras and Convolvulus oxyphyllus are native species of arid and semi-arid desert regions, including Kuwait. They are inherently adapted to extreme conditions characterized by high temperature, prolonged drought and limited rainfall. They contribute significantly, to restore the ecosystem by providing food and shelter for wildlife. Despite their ecological importance, seed germination in both species is constrained by dormancy, and natural recruitment of seedlings is limited by narrow and unpredictable rainfall windows, severely restricting opportunities for establishment. In this context, seed priming is an effective pre-sowing technique that enables uniform and rapid germination within the short window of favorable conditions. This study aims to identify the optimal seed-priming techniques for these two keystone native species and to assess the effect of priming on seed longevity. The seeds were scarified to remove the physical dormancy prior to priming treatments. Relative seed longevity was measured for primed seeds through artificial ageing and analyzed using probit analysis and analysis of variance. Results showed that hormonal priming [(mechanical scarification + 100 ppm gibberellic acid (GA3)] for six hours and hydropriming (mechanical scarification + soaking in water) for 8 hours (h) were effective treatments among the tested priming treatments for V. pachyceras. The estimated p50 value (i.e., number of days to lose seed viability to 50%) was 22 days for hormonal priming and 21 days for hydropriming. Mechanical scarification followed by one and half hour of hydropriming was the most effective in C. oxyphyllus (p50 = 74 days). Based on these results, the longevity of V. pachyceras and C. oxyphyllus could be considered medium. Further studies are needed to determine the effectiveness of seed priming on additional ecologically and economically important native species to achieve success in revegetation programs.
ER -