Characterization of post-mitotic DNA synthesis and its regulation in human cells
- 1Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland
- 2Molecular Life Sciences PhD Program, Life Science Zurich Graduate School, Zurich, Switzerland
Abstract
DNA replication stress promotes cancer development and is associated with DNA synthesis beyond S-phase. Replication stress-induced DNA lesions and under-replicated DNA can persist into G2 and mitosis, where they undergo mitotic DNA synthesis (MiDAS). After cell division, inherited DNA lesions are protected by genome caretaker proteins and show signs of post-mitotic DNA synthesis (post-MiDAS) in G1. The genomic regions that undergo post-MiDAS, the cellular factors involved, and the implications for genome integrity remain poorly understood. Here, we provide evidence for post-MiDAS activity at centromeres and telomeres in cancer cells employing alternative lengthening of telomeres (ALT) and present data suggesting that a subset of these fragile genomic regions can cluster during post-MiDAS in G1. We further show that post-MiDAS is promoted by local SUMOylation and BLM recruitment into ALT-associated PML bodies (APBs) and involves DNA damage tolerance factors and proteins associated with ALT and MiDAS. Consistent with G1-specific regulation, complementary quantitative proteomics and targeted siRNA screening reveal that APC/C, together with its G1 co-activator CDH1 but independent of its mitotic co-activator CDC20, restrains excessive post-MiDAS in G1. Finally, we show that CDH1 depletion selectively impairs the survival of ALT-positive cancer cell lines, indicating that post-MiDAS might represent a potential ALT-specific vulnerability.
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BibTeX
@article{vornberger2026characterization,
title = {{Characterization of post-mitotic DNA synthesis and its regulation in human cells}},
author = {Julia Vornberger and Ralph Imhof and Virginia Pasti and Beatrice Bianchi and Mingxuan Wei and Jeannette Alisha Straw and Sandhya Manohar and Matthias Altmeyer},
journal = {Nature Communications},
year = {2026},
month = sep,
doi = {10.1038/s41467-026-78035-5},
url = {https://doi.org/10.1038/s41467-026-78035-5},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Characterization of post-mitotic DNA synthesis and its regulation in human cells
AU - Julia Vornberger
AU - Ralph Imhof
AU - Virginia Pasti
AU - Beatrice Bianchi
AU - Mingxuan Wei
AU - Jeannette Alisha Straw
AU - Sandhya Manohar
AU - Matthias Altmeyer
PY - 2026
DA - 2026/09/28
JO - Nature Communications
DO - 10.1038/s41467-026-78035-5
UR - https://doi.org/10.1038/s41467-026-78035-5
AB - DNA replication stress promotes cancer development and is associated with DNA synthesis beyond S-phase. Replication stress-induced DNA lesions and under-replicated DNA can persist into G2 and mitosis, where they undergo mitotic DNA synthesis (MiDAS). After cell division, inherited DNA lesions are protected by genome caretaker proteins and show signs of post-mitotic DNA synthesis (post-MiDAS) in G1. The genomic regions that undergo post-MiDAS, the cellular factors involved, and the implications for genome integrity remain poorly understood. Here, we provide evidence for post-MiDAS activity at centromeres and telomeres in cancer cells employing alternative lengthening of telomeres (ALT) and present data suggesting that a subset of these fragile genomic regions can cluster during post-MiDAS in G1. We further show that post-MiDAS is promoted by local SUMOylation and BLM recruitment into ALT-associated PML bodies (APBs) and involves DNA damage tolerance factors and proteins associated with ALT and MiDAS. Consistent with G1-specific regulation, complementary quantitative proteomics and targeted siRNA screening reveal that APC/C, together with its G1 co-activator CDH1 but independent of its mitotic co-activator CDC20, restrains excessive post-MiDAS in G1. Finally, we show that CDH1 depletion selectively impairs the survival of ALT-positive cancer cell lines, indicating that post-MiDAS might represent a potential ALT-specific vulnerability.
SN - 2041-1723
ER -