Skip to content
DraconiticResearch
Search Sources API
197,768 studies31 sourcesUpdated 44 min ago
Peer-reviewedCC BY-NC-NDJournal of Ginseng Research

Chemically profiled total saponins of

  • Mei Yang1
  • Jingkai Zhang2
  • Xiang Guo3
  • Yushen Chen2
  • Zhigang Mei4
  • Xiaoqiang Hou5
  • Zhitao Feng
5 affiliations
  1. 1Basic Medical College of China Three Gorges University, Yichang, Hubei, 443002, China; Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by the State Administration of Traditional Chinese Medicine, College of Medicine and Health Science of China Three Gorges University, Yichang, Hubei, 443002, China
  2. 2Third-Grade Pharmacological Laboratory on Chinese Medicine Approved by the State Administration of Traditional Chinese Medicine, College of Medicine and Health Science of China Three Gorges University, Yichang, Hubei, 443002, China
  3. 3The Second Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China
  4. 4The Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China
  5. 5Institute of Rheumatology, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei, 443003, China; Corresponding author. The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei, 443003, China.
Read full textPDFDOICite

Abstract

Background: Rheumatoid arthritis (RA)-associated bone erosion is largely driven by excessive osteoclast differentiation and activation. Total saponins of Panax japonicus C.A. Meyer (TSPJs), a ginseng-derived botanical fraction, show anti-inflammatory activity in RA-related settings, but their effects on osteoclast-mediated bone destruction remain unclear. Methods: Transcriptomic datasets from RA synovial tissues and RANKL-induced osteoclast differentiation were integrated to identify shared pathogenic signals. TSPJs were chemically profiled by HPLC and evaluated in collagen-induced arthritis mice and RANKL-stimulated RAW264.7 cells. Osteoclast formation and function were assessed by TRAP staining, F-actin ring analysis, gene expression, bone resorption assays, histopathology, radiography, and serological measurements. Network pharmacology, molecular docking, CETSA, immunofluorescence, Western blotting, and immunohistochemistry were used to explore candidate mechanisms. Results: Integrated bioinformatics highlighted NF-κB-related signaling as a shared pathway linking RA and osteoclastogenesis. HPLC identified four representative saponins: araloside A, chikusetsusaponin IVa, ginsenoside Rg2, and ginsenoside Ro. In CIA mice, TSPJs reduced arthritis severity, improved joint pathology, lowered osteoclast-associated serum markers, and attenuated radiographic and histological bone erosion. In vitro, TSPJs inhibited RANKL-induced osteoclast differentiation, disrupted F-actin rings, downregulated osteoclast-related genes, and reduced bone resorption. The overall evidence was consistent with modulation of SRC/TRAF6/NF-κB signaling, while docking and CETSA supported SRC/NFATc1/FOS-related nodes as candidates for further validation. Conclusion: TSPJs attenuated RA-associated bone erosion and restrained osteoclastogenesis, supporting further evaluation of this Panax japonicus -derived saponin preparation.

Related

  1. Total secondary saponins from Anemone raddeana suppress pancreatic cancer via Caspase-3/GSDME-mediated pyroptosis.
    Luanwei Han, Mingshu Li, Qiao Li +4
    Chinese herbal medicinesPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMCPMC
  2. Saponins induce lipid-dependent structural heterogeneity and modulate membrane organization in phospholipid bilayers
    Żaneta Polańska, Zuzanna Pietralik-Molińska, Adam Grzywaczyk +6
    Scientific ReportsPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationCrossref
  3. Molecular Mechanisms of Natural Saponins in Treating Acute Lung Injury: Systemic Regulatory Roles Involving Multiple Cell Types and Signaling Pathways
    Zicheng Mei, Yuji Pan, Changshun Chen +6
    Biological and Pharmaceutical BulletinPeer-reviewedCC BY-NCFull textMolecular biology & gene regulationJ-STAGE
  4. Microwave-assisted natural deep eutectic solvents extraction of saponins from the fibrous roots of American ginseng: process optimization, antioxidant activity, and metabolomics analysis
    Ping Yu, Haiyan Wang, Xin Wang +4
    NPJ Science of FoodPeer-reviewedCC BY-NC-ND 4.0Full text2 citedMolecular biology & gene regulationPMC
  5. Triterpenoid saponins from the aerial parts of Phytolacca americana and their anti-influenza A virus activity
    Shuo Tian, Jisu Park, You Jin Kim +4
    Royal Society Open SciencePeer-reviewedCC BY 4.0Full textChemical biologyMedicinal chemistry & drug discoveryCrossrefEurope PMCPMC

Cite

BibTeXRIS
BibTeX
@article{yang2026chemically,
  title = {{Chemically profiled total saponins of}},
  author = {Mei Yang and Jingkai Zhang and Xiang Guo and Yushen Chen and Zhigang Mei and Xiaoqiang Hou and Zhitao Feng},
  journal = {Journal of Ginseng Research},
  year = {2026},
  month = jul,
  doi = {10.1016/j.jgr.2026.101113},
  url = {https://doi.org/10.1016/j.jgr.2026.101113},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Chemically profiled total saponins of
AU  - Mei Yang
AU  - Jingkai Zhang
AU  - Xiang Guo
AU  - Yushen Chen
AU  - Zhigang Mei
AU  - Xiaoqiang Hou
AU  - Zhitao Feng
PY  - 2026
DA  - 2026/07/11
JO  - Journal of Ginseng Research
DO  - 10.1016/j.jgr.2026.101113
UR  - https://doi.org/10.1016/j.jgr.2026.101113
AB  - Background: Rheumatoid arthritis (RA)-associated bone erosion is largely driven by excessive osteoclast differentiation and activation. Total saponins of Panax japonicus C.A. Meyer (TSPJs), a ginseng-derived botanical fraction, show anti-inflammatory activity in RA-related settings, but their effects on osteoclast-mediated bone destruction remain unclear. Methods: Transcriptomic datasets from RA synovial tissues and RANKL-induced osteoclast differentiation were integrated to identify shared pathogenic signals. TSPJs were chemically profiled by HPLC and evaluated in collagen-induced arthritis mice and RANKL-stimulated RAW264.7 cells. Osteoclast formation and function were assessed by TRAP staining, F-actin ring analysis, gene expression, bone resorption assays, histopathology, radiography, and serological measurements. Network pharmacology, molecular docking, CETSA, immunofluorescence, Western blotting, and immunohistochemistry were used to explore candidate mechanisms. Results: Integrated bioinformatics highlighted NF-κB-related signaling as a shared pathway linking RA and osteoclastogenesis. HPLC identified four representative saponins: araloside A, chikusetsusaponin IVa, ginsenoside Rg2, and ginsenoside Ro. In CIA mice, TSPJs reduced arthritis severity, improved joint pathology, lowered osteoclast-associated serum markers, and attenuated radiographic and histological bone erosion. In vitro, TSPJs inhibited RANKL-induced osteoclast differentiation, disrupted F-actin rings, downregulated osteoclast-related genes, and reduced bone resorption. The overall evidence was consistent with modulation of SRC/TRAF6/NF-κB signaling, while docking and CETSA supported SRC/NFATc1/FOS-related nodes as candidates for further validation. Conclusion: TSPJs attenuated RA-associated bone erosion and restrained osteoclastogenesis, supporting further evaluation of this Panax japonicus -derived saponin preparation.
SN  - 1226-8453
SN  - 2093-4947
ER  -