Neuromedin B Regulates Cell Proliferation, Apoptosis and Steroidogenesis of Porcine Ovarian Granulosa Cells via NMBR.
- 1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
- 2Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
- 3Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Abstract
Granulosa cells (GCs) critically govern follicular development through the coordinated regulation of proliferation, apoptosis, and steroidogenesis. Neuromedin B (NMB), a local regulatory peptide, has been implicated in female reproductive processes. However, its precise effects on porcine GCs remain to be elucidated. In this study, we characterized the ovarian distribution of NMBR, investigated the effects of NMB on GC function, and further explored the involvement of NMBR in these effects using the NMBR antagonist PD168368. The results showed that higher NMBR expression was observed in follicles of larger diameter. NMB treatment differentially regulated the functional activities of porcine ovarian GCs. Specifically, certain concentrations of NMB significantly increased cell viability and altered the expression levels of the proliferation-related markers Cyclin B1 and PCNA; reduced the cell apoptosis rate and modulated the expression levels of apoptosis-related genes and proteins (Bax, Bcl-2, and Caspase-3); and affected steroidogenic responses, including increased E₂ concentrations at selected concentrations while altering expression of STAR, CYP11A1, HSD3B1, and CYP19A1. Importantly, several of these effects were attenuated by PD168368 treatment. Collectively, these findings suggest that NMB may regulate multiple functions of porcine with the involvement of NMBR. These findings provide a theoretical basis for elucidating the neuroendocrine mechanisms underlying porcine follicular development and for further investigating the potential role of NMB/NMBR signaling in porcine follicular physiology.
Related
- Cell-intrinsic steroidogenesis regulates mast cell function
- lncRNA-5583 Modulates Porcine Granulosa Cell Proliferation Through a miR-193a-5p/ACVR1-Associated Mechanism.
- Cell size asymmetry regulates neural stem cell proliferation and lineage expansion in the Drosophila brain
- Selenium-Enriched Rapeseed Extract Synergizes with Chemotherapy Drug Cisplatin in Inhibiting Proliferation and Promoting Apoptosis of Colorectal Cancer Cells
- LncRNA PARD3-AS1 regulates the proliferation and migration of human umbilical vein endothelial cells in atherosclerosis by targeting miR-668-3p
BibTeX
@article{ma2026neuromedin,
title = {{Neuromedin B Regulates Cell Proliferation, Apoptosis and Steroidogenesis of Porcine Ovarian Granulosa Cells via NMBR.}},
author = {Zhiyu Ma and Hongxia Li and Guangzhen Chen and Jia Li and Dandan Wang and Yiwen Wang and Sheng Yang and Qi Liu and Fenglei Chen and Shifeng Pan},
journal = {Veterinary sciences},
year = {2026},
month = oct,
doi = {10.3390/vetsci13101088},
url = {https://europepmc.org/article/MED/42944182},
note = {CC BY},
}
RIS
TY - JOUR
TI - Neuromedin B Regulates Cell Proliferation, Apoptosis and Steroidogenesis of Porcine Ovarian Granulosa Cells via NMBR.
AU - Zhiyu Ma
AU - Hongxia Li
AU - Guangzhen Chen
AU - Jia Li
AU - Dandan Wang
AU - Yiwen Wang
AU - Sheng Yang
AU - Qi Liu
AU - Fenglei Chen
AU - Shifeng Pan
PY - 2026
DA - 2026/10/14
JO - Veterinary sciences
DO - 10.3390/vetsci13101088
UR - https://europepmc.org/article/MED/42944182
AB - Granulosa cells (GCs) critically govern follicular development through the coordinated regulation of proliferation, apoptosis, and steroidogenesis. Neuromedin B (NMB), a local regulatory peptide, has been implicated in female reproductive processes. However, its precise effects on porcine GCs remain to be elucidated. In this study, we characterized the ovarian distribution of NMBR, investigated the effects of NMB on GC function, and further explored the involvement of NMBR in these effects using the NMBR antagonist PD168368. The results showed that higher NMBR expression was observed in follicles of larger diameter. NMB treatment differentially regulated the functional activities of porcine ovarian GCs. Specifically, certain concentrations of NMB significantly increased cell viability and altered the expression levels of the proliferation-related markers Cyclin B1 and PCNA; reduced the cell apoptosis rate and modulated the expression levels of apoptosis-related genes and proteins (Bax, Bcl-2, and Caspase-3); and affected steroidogenic responses, including increased E₂ concentrations at selected concentrations while altering expression of STAR, CYP11A1, HSD3B1, and CYP19A1. Importantly, several of these effects were attenuated by PD168368 treatment. Collectively, these findings suggest that NMB may regulate multiple functions of porcine with the involvement of NMBR. These findings provide a theoretical basis for elucidating the neuroendocrine mechanisms underlying porcine follicular development and for further investigating the potential role of NMB/NMBR signaling in porcine follicular physiology.
SN - 2306-7381
ER -