Antifungal susceptibility and genomic profile associated with biosynthetic gene clusters of bovine mastitis-derived Aspergillus fumigatus DAH1043FM
- 1Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University , ,
- 2Gujarat Biotechnology Research Center, Department of Science and Technology , ,
- 3Office of Deputy Director of Animal Husbandry, FMD Typing Scheme, Pashupalan Sankul , ,
Abstract
The most frequently isolated fungus from mycotic mastitis in cattle is Aspergillus fumigatus , which can cause infection by germinating on the animal's surface to form hyphae that penetrate epithelial and endothelial barriers and initiate mastitis. In this study, the fungal strain DAH1043FM was isolated from the milk of a cow with mastitis and identified as Aspergillus fumigatus by internal transcribed spacer (ITS) region sequencing. Species identity was further confirmed by whole-genome Average Nucleotide Identity (ANI) analysis. A. fumigatus DAH1043FM showed minimum inhibitory concentration (MIC) values of 0.5 μg/mL for amphotericin B and itraconazole, 0.12 μg/mL for posaconazole and voriconazole, 128 μg/mL for fluconazole, and >8 μg/mL for caspofungin, micafungin, and anidulafungin. Comparative Whole-genome sequencing (WGS) with two reference genomes of A. fumigatus , Af293 and A1163, was used to identify genetic variants, such as single nucleotide polymorphisms (SNPs) and other mutations, in genes associated with antifungal resistance, including those involved in drug efflux, ergosterol biosynthesis, echinocandin target pathways, and other resistance-related processes. Furthermore, genome mining identified biosynthetic gene clusters (BGCs) showing substantial similarity to characterized pathways associated with fumiquinazoline (100%), gliotoxin (84%), fumagillin (80%), and trypacidin (71%). These results demonstrate that strain DAH1043FM carries predicted genetic variants in target pathways while exhibiting wild-type susceptibility to azoles alongside off-scale endpoints for echinocandins. Genome analysis of strain DAH1043FM identified predicted secondary metabolite BGCs, highlighting the value of genome-level surveillance for fungal isolates recovered from bovine milk.
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BibTeX
@article{prajapati2026antifungal,
title = {{Antifungal susceptibility and genomic profile associated with biosynthetic gene clusters of bovine mastitis-derived Aspergillus fumigatus DAH1043FM}},
author = {Abhishek Prajapati and Pooja Parmar and Monica Chavan and Vartika Chandra and Satyamitra Shekh and Ashish Patel and Pritesh Sabara and Amit Kanani and Snehal Bagatharia and Chaitanya Kumar Jha},
journal = {Frontiers in microbiology},
year = {2026},
month = sep,
doi = {10.3389/fmicb.2026.1928624},
url = {https://europepmc.org/article/PMC/PMC13661572},
note = {CC BY},
}
RIS
TY - JOUR
TI - Antifungal susceptibility and genomic profile associated with biosynthetic gene clusters of bovine mastitis-derived Aspergillus fumigatus DAH1043FM
AU - Abhishek Prajapati
AU - Pooja Parmar
AU - Monica Chavan
AU - Vartika Chandra
AU - Satyamitra Shekh
AU - Ashish Patel
AU - Pritesh Sabara
AU - Amit Kanani
AU - Snehal Bagatharia
AU - Chaitanya Kumar Jha
PY - 2026
DA - 2026/09/30
JO - Frontiers in microbiology
DO - 10.3389/fmicb.2026.1928624
UR - https://europepmc.org/article/PMC/PMC13661572
AB - The most frequently isolated fungus from mycotic mastitis in cattle is Aspergillus fumigatus , which can cause infection by germinating on the animal's surface to form hyphae that penetrate epithelial and endothelial barriers and initiate mastitis. In this study, the fungal strain DAH1043FM was isolated from the milk of a cow with mastitis and identified as Aspergillus fumigatus by internal transcribed spacer (ITS) region sequencing. Species identity was further confirmed by whole-genome Average Nucleotide Identity (ANI) analysis. A. fumigatus DAH1043FM showed minimum inhibitory concentration (MIC) values of 0.5 μg/mL for amphotericin B and itraconazole, 0.12 μg/mL for posaconazole and voriconazole, 128 μg/mL for fluconazole, and >8 μg/mL for caspofungin, micafungin, and anidulafungin. Comparative Whole-genome sequencing (WGS) with two reference genomes of A. fumigatus , Af293 and A1163, was used to identify genetic variants, such as single nucleotide polymorphisms (SNPs) and other mutations, in genes associated with antifungal resistance, including those involved in drug efflux, ergosterol biosynthesis, echinocandin target pathways, and other resistance-related processes. Furthermore, genome mining identified biosynthetic gene clusters (BGCs) showing substantial similarity to characterized pathways associated with fumiquinazoline (100%), gliotoxin (84%), fumagillin (80%), and trypacidin (71%). These results demonstrate that strain DAH1043FM carries predicted genetic variants in target pathways while exhibiting wild-type susceptibility to azoles alongside off-scale endpoints for echinocandins. Genome analysis of strain DAH1043FM identified predicted secondary metabolite BGCs, highlighting the value of genome-level surveillance for fungal isolates recovered from bovine milk.
SN - 1664-302X
ER -