Integrated genomic and phenotypic analysis of intra-clade variation in Candida auris from a New York City tertiary hospital
4 affiliations
- 1Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York, USA
- 2Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, Texas, USA
- 3Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA
- 4Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA
Abstract
Candida (Candidozyma) auris (C. auris) poses an urgent public health threat due to its widespread emergence, antimicrobial resistance, and ability to cause severe infections and outbreaks associated with high mortality rates. In this study, we phenotypically and genotypically characterized antifungal susceptibility profiles and used whole-genome sequencing (WGS) to assess overall genetic diversity and to identify clonal clusters. From July 2020 to December 2025, a total of 118 C. auris isolates from 97 patients were collected at a tertiary hospital in New York City. Antifungal susceptibility testing and WGS were performed on all clinical isolates to characterize their clade-specific antifungal resistance genes and intra-clade genetic variations. Ninety-nine percentage of the C. auris isolates demonstrated phenotypic fluconazole resistance. Resistance to echinocandins was observed in 7.6% (9/118) of isolates, and resistance to amphotericin B was found in 54% (64/118). WGS confirmed that all 118 isolates belonged to the CDC-defined Clade Ic. Azole resistance mutations in the ERG11 and CDR1 genes were detected in 100% (118/118) of the isolates, and mutations in the TAC1B gene were present in 92.4% (109/118) of the isolates, including one case with a unique set of mutations (ERG11-Y132F, CDR1-E709D, TAC1B-A583S). Mutations in the FKS1 gene were identified in 5.9% (7/118) of isolates though these were not consistently linked to echinocandin resistance. Genomic analysis also revealed that individual patients frequently carried the same strain throughout the study. Furthermore, multiple strains emerged simultaneously across the hospital each year. This suggests that numerous patients were likely already colonized upon admission, rather than acquiring the pathogen through intra-hospital transmission. The presence of multiple C. auris strains with varying resistance patterns poses significant therapeutic challenges. Genomic typing can help hospitals understand the diversity of their C. auris isolates to track their spread, identify potential outbreaks, and ensure the effectiveness of infection control measures.
IMPORTANCE: Candida (Candidozyma) auris is an emerging, multidrug-resistant fungal pathogen designated by the Centers for Disease Control and Prevention (CDC) as an urgent public threat. Traditionally, C. auris infections have been considered predominantly nosocomial, with outbreaks typically attributed to a single clonal lineage spreading within healthcare facilities. However, the genomic analysis performed in our study identified multiple distinct clusters of genetic variations within a single clade, suggesting that infections were not derived from a single source. This genomic diversity indicates that many patients may have acquired C. auris prior to hospital admission. Nevertheless, intra-hospital transmission was evident, as patient-to-patient spread facilitated the dissemination of various clonal lineages within the healthcare environment. These findings underscore the complex epidemiology of C. auris and highlight the urgent need for stringent infection control measures both within and beyond hospital settings.
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BibTeX
@article{satia2026integrated,
title = {{Integrated genomic and phenotypic analysis of intra-clade variation in Candida auris from a New York City tertiary hospital}},
author = {Shivani Satia and Jennifer Pfeiffer and Abdullah Kilic and E. Yoko Furuya and Marie C. Smithgall and Mahesh Mansukhani and Susan Hsiao and Jun Shi and Rinki Kumar and Daniel A. Green and Gregory J. Berry and Fann Wu},
journal = {Microbiology Spectrum},
year = {2026},
month = sep,
doi = {10.1128/spectrum.01695-26},
url = {https://doi.org/10.1128/spectrum.01695-26},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Integrated genomic and phenotypic analysis of intra-clade variation in Candida auris from a New York City tertiary hospital
AU - Shivani Satia
AU - Jennifer Pfeiffer
AU - Abdullah Kilic
AU - E. Yoko Furuya
AU - Marie C. Smithgall
AU - Mahesh Mansukhani
AU - Susan Hsiao
AU - Jun Shi
AU - Rinki Kumar
AU - Daniel A. Green
AU - Gregory J. Berry
AU - Fann Wu
PY - 2026
DA - 2026/09/24
JO - Microbiology Spectrum
DO - 10.1128/spectrum.01695-26
UR - https://doi.org/10.1128/spectrum.01695-26
AB - Candida (Candidozyma) auris (C. auris) poses an urgent public health threat due to its widespread emergence, antimicrobial resistance, and ability to cause severe infections and outbreaks associated with high mortality rates. In this study, we phenotypically and genotypically characterized antifungal susceptibility profiles and used whole-genome sequencing (WGS) to assess overall genetic diversity and to identify clonal clusters. From July 2020 to December 2025, a total of 118 C. auris isolates from 97 patients were collected at a tertiary hospital in New York City. Antifungal susceptibility testing and WGS were performed on all clinical isolates to characterize their clade-specific antifungal resistance genes and intra-clade genetic variations. Ninety-nine percentage of the C. auris isolates demonstrated phenotypic fluconazole resistance. Resistance to echinocandins was observed in 7.6% (9/118) of isolates, and resistance to amphotericin B was found in 54% (64/118). WGS confirmed that all 118 isolates belonged to the CDC-defined Clade Ic. Azole resistance mutations in the ERG11 and CDR1 genes were detected in 100% (118/118) of the isolates, and mutations in the TAC1B gene were present in 92.4% (109/118) of the isolates, including one case with a unique set of mutations (ERG11-Y132F, CDR1-E709D, TAC1B-A583S). Mutations in the FKS1 gene were identified in 5.9% (7/118) of isolates though these were not consistently linked to echinocandin resistance. Genomic analysis also revealed that individual patients frequently carried the same strain throughout the study. Furthermore, multiple strains emerged simultaneously across the hospital each year. This suggests that numerous patients were likely already colonized upon admission, rather than acquiring the pathogen through intra-hospital transmission. The presence of multiple C. auris strains with varying resistance patterns poses significant therapeutic challenges. Genomic typing can help hospitals understand the diversity of their C. auris isolates to track their spread, identify potential outbreaks, and ensure the effectiveness of infection control measures. IMPORTANCE: Candida (Candidozyma) auris is an emerging, multidrug-resistant fungal pathogen designated by the Centers for Disease Control and Prevention (CDC) as an urgent public threat. Traditionally, C. auris infections have been considered predominantly nosocomial, with outbreaks typically attributed to a single clonal lineage spreading within healthcare facilities. However, the genomic analysis performed in our study identified multiple distinct clusters of genetic variations within a single clade, suggesting that infections were not derived from a single source. This genomic diversity indicates that many patients may have acquired C. auris prior to hospital admission. Nevertheless, intra-hospital transmission was evident, as patient-to-patient spread facilitated the dissemination of various clonal lineages within the healthcare environment. These findings underscore the complex epidemiology of C. auris and highlight the urgent need for stringent infection control measures both within and beyond hospital settings.
SN - 2165-0497
ER -