Low MATR3 Expression Is Associated with Poor Prognosis in Endometrial Carcinoma: An Integrated Immunohistochemical and Transcriptomic Analysis
- 1Department of Clinical Pathomorphology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-094 Bydgoszcz, Poland
- 2Department of Histology and Embryology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-092 Bydgoszcz, Poland
Abstract
Endometrial carcinoma (EC) is the most common malignancy of the female genital tract, yet reliable prognostic biomarkers remain limited. Matrin-3 (MATR3), a nuclear matrix protein involved in genome stability and RNA processing, has been implicated in various cancers, but its role in EC has not been described. Here, we performed an integrated analysis combining immunohistochemical evaluation of MATR3 protein expression in a retrospective institutional cohort of 112 patients with EC and in silico analysis of MATR3 mRNA expression in 172 TCGA cases. Immunohistochemical analysis revealed nuclear MATR3 expression in both EC and normal endometrial tissues, with significantly lower protein levels in EC compared to control tissues (p < 0.0001). MATR3 mRNA was significantly reduced in EC tumour tissues compared to adjacent normal tissues (p < 0.0001). Low MATR3 protein expression was associated with shorter OS after adjustment for age, histological grade, pT, pN and pM in an LVSI-stratified Cox model (HR = 2.296; 95% CI: 1.284–4.104; p = 0.0050). Low MATR3 mRNA expression was associated with improved overall survival in TCGA (median OS = 110.1 months; HR = 2.174; 95% CI: 1.066–4.43; p = 0.0303). Co-expression analysis identified BCLAF1 and TOP2B as key positive correlates, while TRAPPC5 and FASTK showed the strongest negative correlations. Genes positively correlated with MATR3 mRNA expression were enriched for nuclear transcription and RNA-processing annotations, whereas negatively correlated genes were enriched for mitochondrial processes. These associations do not establish a mechanistic link between MATR3 and pathway activity. Our findings support further investigation of MATR3 as a candidate prognostic marker in EC, with independent validation and functional studies required.
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BibTeX
@article{podemski2026matr3,
title = {{Low MATR3 Expression Is Associated with Poor Prognosis in Endometrial Carcinoma: An Integrated Immunohistochemical and Transcriptomic Analysis}},
author = {Jonasz Podemski and Dariusz Grzanka and Edyta Podemska and Natalia Skoczylas-Makowska and Dominika Jerka and Maciej Gagat and Paulina Antosik},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27219708},
url = {https://www.mdpi.com/1422-0067/27/21/9708},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Low MATR3 Expression Is Associated with Poor Prognosis in Endometrial Carcinoma: An Integrated Immunohistochemical and Transcriptomic Analysis
AU - Jonasz Podemski
AU - Dariusz Grzanka
AU - Edyta Podemska
AU - Natalia Skoczylas-Makowska
AU - Dominika Jerka
AU - Maciej Gagat
AU - Paulina Antosik
PY - 2026
DA - 2026/10/29
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219708
UR - https://www.mdpi.com/1422-0067/27/21/9708
AB - Endometrial carcinoma (EC) is the most common malignancy of the female genital tract, yet reliable prognostic biomarkers remain limited. Matrin-3 (MATR3), a nuclear matrix protein involved in genome stability and RNA processing, has been implicated in various cancers, but its role in EC has not been described. Here, we performed an integrated analysis combining immunohistochemical evaluation of MATR3 protein expression in a retrospective institutional cohort of 112 patients with EC and in silico analysis of MATR3 mRNA expression in 172 TCGA cases. Immunohistochemical analysis revealed nuclear MATR3 expression in both EC and normal endometrial tissues, with significantly lower protein levels in EC compared to control tissues (p < 0.0001). MATR3 mRNA was significantly reduced in EC tumour tissues compared to adjacent normal tissues (p < 0.0001). Low MATR3 protein expression was associated with shorter OS after adjustment for age, histological grade, pT, pN and pM in an LVSI-stratified Cox model (HR = 2.296; 95% CI: 1.284–4.104; p = 0.0050). Low MATR3 mRNA expression was associated with improved overall survival in TCGA (median OS = 110.1 months; HR = 2.174; 95% CI: 1.066–4.43; p = 0.0303). Co-expression analysis identified BCLAF1 and TOP2B as key positive correlates, while TRAPPC5 and FASTK showed the strongest negative correlations. Genes positively correlated with MATR3 mRNA expression were enriched for nuclear transcription and RNA-processing annotations, whereas negatively correlated genes were enriched for mitochondrial processes. These associations do not establish a mechanistic link between MATR3 and pathway activity. Our findings support further investigation of MATR3 as a candidate prognostic marker in EC, with independent validation and functional studies required.
SN - 1422-0067
ER -