Lipid Extract from Ulva ohnoi Algae: Chemical Characterization, Antibacterial Potential, and Cytotoxicity in the Search for New Agents Against MDR Infections.

@article{jara2026lipid,
  title = {{Lipid Extract from Ulva ohnoi Algae: Chemical Characterization, Antibacterial Potential, and Cytotoxicity in the Search for New Agents Against MDR Infections.}},
  author = {Marisa Castro Jara and Diego Serrasol do Amaral and Leonardo Werner and Eduardo Bastos and Luana de Azevedo Aimi and Nathalia Stark Pedra and Vithor Parada Garcia and Matheus Pereira de Albuquerque and Clarissa Piccinin Frizzo and Milena de Nardin and Wellington da Rocha da Silva and Silvia de Oliveira Hübner and Rodrigo de Almeida Vaucher and Cláudio Martins Pereira de Pereira and Patrícia da Silva Nascente},
  journal = {Marine biotechnology (New York, N.Y.)},
  year = {2026},
  month = oct,
  doi = {10.1007/s10126-026-10717-z},
  url = {https://europepmc.org/article/MED/42891715},
  note = {CC BY},
}

TY  - JOUR
TI  - Lipid Extract from Ulva ohnoi Algae: Chemical Characterization, Antibacterial Potential, and Cytotoxicity in the Search for New Agents Against MDR Infections.
AU  - Marisa Castro Jara
AU  - Diego Serrasol do Amaral
AU  - Leonardo Werner
AU  - Eduardo Bastos
AU  - Luana de Azevedo Aimi
AU  - Nathalia Stark Pedra
AU  - Vithor Parada Garcia
AU  - Matheus Pereira de Albuquerque
AU  - Clarissa Piccinin Frizzo
AU  - Milena de Nardin
AU  - Wellington da Rocha da Silva
AU  - Silvia de Oliveira Hübner
AU  - Rodrigo de Almeida Vaucher
AU  - Cláudio Martins Pereira de Pereira
AU  - Patrícia da Silva Nascente
PY  - 2026
DA  - 2026/10/17
JO  - Marine biotechnology (New York, N.Y.)
DO  - 10.1007/s10126-026-10717-z
UR  - https://europepmc.org/article/MED/42891715
AB  - Antimicrobial resistance is a critical global concern. This study explored the lipid extract (LE) of Ulva ohnoi regarding its chemical composition, antibacterial activity, and cytotoxicity against multidrug-resistant (MDR) microorganisms. The LE was characterized by GC-MS, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Antibacterial efficacy was determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against MDR isolates: Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and coagulase-negative Staphylococci (CoNS). Cytotoxicity was evaluated in murine fibroblast (L929) and bovine renal fibroblast (MDBK) cells using MTT, Neutral Red (NR), Lactate Dehydrogenase (LDH), and hemolytic activity (HA) assays. Lipid characterization revealed palmitic acid (63.62%) and oleic acid (21.01%) as major components. TGA indicated 88% mass loss, and DSC showed no amorphous/crystalline domains. LE showed favorable MIC values against S. aureus and CoNS (1.33 mg/mL) and against A. baumannii and E. coli (2 mg/mL), indicating moderate antibacterial activity typical of an unfractionated crude extract. MBC results indicated bacteriostatic activity (1-8 mg/mL). The extract was cytotoxic to L929 and MDBK cells at concentrations close to its MIC (IC₅₀ 0.25-1.95 mg/mL) but well tolerated by blood cells (HA IC₅₀ 4.705 mg/mL). U. ohnoi LE extends, rather than establishes, the previously reported antibacterial potential of Ulva lipids through comprehensive chemical characterization and MDR-isolate evaluation. However, low selectivity indices (SI < 10) indicate that the LE does not yet present an adequate therapeutic window, reinforcing the need for bioassay-guided purification. Nevertheless, it is a starting point for developing new antibacterial agents.
SN  - 1436-2228
SN  - 1436-2236
ER  -