GelMA ‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
Abstract
Myeloid sarcoma (MS) is a rare extramedullary manifestation of acute myeloid leukemia (AML). Conventional two-dimensional cultures fail to replicate the constraints of the MS microenvironment. We demonstrated that 5% gelatin methacrylate (GelMA) hydrogels provide a tunable three-dimensional matrix for in vitro modeling of MS. GelMA hydrogels exhibited tissue-like stiffness and oxygen diffusion properties, supporting the formation of viable spheroids from myeloid leukemia cell lines. The 3D context induced G1 cell cycle arrest and apoptotic progression, reflecting dormancy at extramedullary sites. RNA sequencing revealed transcriptional reprogramming in GelMA-embedded spheroids, with an enrichment in ECM remodeling and metabolic pathways. Furthermore, transcriptomic profiles from 3D-cultured cells aligned with patient-derived MS samples, validating the model's relevance to MS. Thus, we present a GelMA-based system which provides a clinically relevant platform for investigating MS biology and screening potential therapeutic strategies.
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BibTeX
@article{germain2026gelma,
title = {{GelMA ‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma}},
author = {Nicolas Germain and Elise Buret and Le Hoang Thanh Nguyen and Marion Cocheteux and Nicolas Touya and Mélanie Dhayer and Romain Dubois and Frank Lafont and Sebastien Janel and Patrice Maboudou and Steve Lancel and Philippe Marchetti},
journal = {FEBS Open Bio},
year = {2026},
month = aug,
doi = {10.1002/2211-5463.70321},
url = {https://hal.science/hal-05764581v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - GelMA ‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
AU - Nicolas Germain
AU - Elise Buret
AU - Le Hoang Thanh Nguyen
AU - Marion Cocheteux
AU - Nicolas Touya
AU - Mélanie Dhayer
AU - Romain Dubois
AU - Frank Lafont
AU - Sebastien Janel
AU - Patrice Maboudou
AU - Steve Lancel
AU - Philippe Marchetti
PY - 2026
DA - 2026/08/13
JO - FEBS Open Bio
DO - 10.1002/2211-5463.70321
UR - https://hal.science/hal-05764581v1
AB - Myeloid sarcoma (MS) is a rare extramedullary manifestation of acute myeloid leukemia (AML). Conventional two-dimensional cultures fail to replicate the constraints of the MS microenvironment. We demonstrated that 5% gelatin methacrylate (GelMA) hydrogels provide a tunable three-dimensional matrix for in vitro modeling of MS. GelMA hydrogels exhibited tissue-like stiffness and oxygen diffusion properties, supporting the formation of viable spheroids from myeloid leukemia cell lines. The 3D context induced G1 cell cycle arrest and apoptotic progression, reflecting dormancy at extramedullary sites. RNA sequencing revealed transcriptional reprogramming in GelMA-embedded spheroids, with an enrichment in ECM remodeling and metabolic pathways. Furthermore, transcriptomic profiles from 3D-cultured cells aligned with patient-derived MS samples, validating the model's relevance to MS. Thus, we present a GelMA-based system which provides a clinically relevant platform for investigating MS biology and screening potential therapeutic strategies.
SN - 2211-5463
SN - 2211-5463
ER -