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Peer-reviewedCC BY-NC-NDSTAR Protocols

A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

  • Nurulain Ho
  • Wei Sheng Yap
  • Guillaume Thibault
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Abstract

The endoplasmic reticulum (ER) stress is defined by the accumulation of unfolded proteins at the ER and perturbation at the ER membrane, known as lipid bilayer stress (LBS). In turn, ER stress triggers the unfolded protein response (UPR) to restore ER homeostasis. Here, we provide a modified protocol based on the synthetic genetic array analysis in Saccharomyces cerevisiae to identify genetic perturbations that induce the UPR by LBS. This method is adaptable to other canonical stress pathways. For complete details on the use and execution of this protocol, please refer to Ho et al. (2020), Jonikas et al. (2009) and Baryshnikova et al. (2010).

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Cite

BibTeXRIS
BibTeX
@article{ho2026highthroughput,
  title = {{A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae}},
  author = {Nurulain Ho and Wei Sheng Yap and Guillaume Thibault},
  journal = {STAR Protocols},
  year = {2026},
  month = sep,
  doi = {10.1016/j.xpro.2021.100868},
  url = {https://hal.science/hal-05770384v1},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae
AU  - Nurulain Ho
AU  - Wei Sheng Yap
AU  - Guillaume Thibault
PY  - 2026
DA  - 2026/09/30
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2021.100868
UR  - https://hal.science/hal-05770384v1
AB  - The endoplasmic reticulum (ER) stress is defined by the accumulation of unfolded proteins at the ER and perturbation at the ER membrane, known as lipid bilayer stress (LBS). In turn, ER stress triggers the unfolded protein response (UPR) to restore ER homeostasis. Here, we provide a modified protocol based on the synthetic genetic array analysis in Saccharomyces cerevisiae to identify genetic perturbations that induce the UPR by LBS. This method is adaptable to other canonical stress pathways. For complete details on the use and execution of this protocol, please refer to Ho et al. (2020), Jonikas et al. (2009) and Baryshnikova et al. (2010).
SN  - 2666-1667
ER  -