Peritumoral SULF1 Transcript Signal Is Associated with Disease-Specific Survival in Oral Squamous Cell Carcinoma
- 1Georgetown University Medical Center
Abstract
The prognostic relevance of peritumoral SULF1 transcript signal in oral squamous cell carcinoma (OSCC) is unclear. We assembled a retrospective cohort of 133 patients with 218 primary-site tissue-microarray cores; 131 patients had evaluable boundary features. Multiplex fluorescence imaging combined RNAscope SULF1, CK-pan, and DAPI. Tumor-stroma boundaries were annotated with the SULF1 channel hidden. We screened 50 exported boundary features across cumulative stromal bands of 35-300 m against recurrence-free survival (RFS), overall survival (OS), and disease-specific survival (DSS), with false-discovery-rate correction. The strongest DSS association was signal SD within 150 m (n = 107; 23 disease-specific deaths; HR = 2.20 per SD; 95% CI, 1.64-2.94; q = 2.1 x 10-). In 50 repeated 5-fold cross-validations with feature selection repeated within training folds, adding a boundary feature to AJCC stage increased mean held-out DSS c-index from 0.613 to 0.754 ({Delta}C = +0.141; 2.5th-97.5th percentiles across repeats, +0.105 to +0.192). These percentiles describe split variability, not confidence intervals. The 150-m SD feature was selected in 39.2% of DSS training folds. The DSS association persisted after adjustment for mean signal, although the measurements were strongly correlated. Peritumoral SULF1 signal dispersion was associated with outcome beyond an ordinal AJCC-stage model; independent validation is required.
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BibTeX
@misc{furletti2026peritumoral,
title = {{Peritumoral SULF1 Transcript Signal Is Associated with Disease-Specific Survival in Oral Squamous Cell Carcinoma}},
author = {G. Furletti and T. C. Guerrero and E. Schrom and B. Davidson and B. Kallakury and R. Goldman},
howpublished = {medRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.09.26364914},
url = {https://www.medrxiv.org/content/10.64898/2026.10.09.26364914v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Peritumoral SULF1 Transcript Signal Is Associated with Disease-Specific Survival in Oral Squamous Cell Carcinoma
AU - G. Furletti
AU - T. C. Guerrero
AU - E. Schrom
AU - B. Davidson
AU - B. Kallakury
AU - R. Goldman
PY - 2026
DA - 2026/10/09
PB - medRxiv
DO - 10.64898/2026.10.09.26364914
UR - https://www.medrxiv.org/content/10.64898/2026.10.09.26364914v1
AB - The prognostic relevance of peritumoral SULF1 transcript signal in oral squamous cell carcinoma (OSCC) is unclear. We assembled a retrospective cohort of 133 patients with 218 primary-site tissue-microarray cores; 131 patients had evaluable boundary features. Multiplex fluorescence imaging combined RNAscope SULF1, CK-pan, and DAPI. Tumor-stroma boundaries were annotated with the SULF1 channel hidden. We screened 50 exported boundary features across cumulative stromal bands of 35-300 m against recurrence-free survival (RFS), overall survival (OS), and disease-specific survival (DSS), with false-discovery-rate correction. The strongest DSS association was signal SD within 150 m (n = 107; 23 disease-specific deaths; HR = 2.20 per SD; 95% CI, 1.64-2.94; q = 2.1 x 10-). In 50 repeated 5-fold cross-validations with feature selection repeated within training folds, adding a boundary feature to AJCC stage increased mean held-out DSS c-index from 0.613 to 0.754 ({Delta}C = +0.141; 2.5th-97.5th percentiles across repeats, +0.105 to +0.192). These percentiles describe split variability, not confidence intervals. The 150-m SD feature was selected in 39.2% of DSS training folds. The DSS association persisted after adjustment for mean signal, although the measurements were strongly correlated. Peritumoral SULF1 signal dispersion was associated with outcome beyond an ordinal AJCC-stage model; independent validation is required.
ER -