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Peer-reviewedCC BYSTAR protocols

Protocol for quantifying mitotic progression in adherent and suspension cells by live-cell imaging.

  • Siamak A Kamranvar1
  1. 1Division of Cancer and Genetics, School of Medicine, Cardiff University, CF14 4XN Cardiff, UK. Electronic address: [email protected].
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Abstract

Live-cell imaging is used to monitor mitotic progression in adherent cells; however, its application to suspension cells remains limited. Here, we present a protocol for quantifying mitotic progression in adherent and suspension cells by monitoring chromatin condensation using the live-cell DNA probe SPY-DNA. We describe steps for cell synchronization, cell plating, SPY-DNA labeling, and live-cell imaging. We then detail procedures for surface plot visualization and intensity profile analysis. This approach enables direct comparison of mitotic progression between adherent and suspension cells. For complete details on the use and execution of this protocol, please refer to Kamranvar et al.¹.

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Cite

BibTeXRIS
BibTeX
@article{kamranvar2026protocol,
  title = {{Protocol for quantifying mitotic progression in adherent and suspension cells by live-cell imaging.}},
  author = {Siamak A Kamranvar},
  journal = {STAR protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104899},
  url = {https://europepmc.org/article/MED/42867999},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Protocol for quantifying mitotic progression in adherent and suspension cells by live-cell imaging.
AU  - Siamak A Kamranvar
PY  - 2026
DA  - 2026/10/13
JO  - STAR protocols
DO  - 10.1016/j.xpro.2026.104899
UR  - https://europepmc.org/article/MED/42867999
AB  - Live-cell imaging is used to monitor mitotic progression in adherent cells; however, its application to suspension cells remains limited. Here, we present a protocol for quantifying mitotic progression in adherent and suspension cells by monitoring chromatin condensation using the live-cell DNA probe SPY-DNA. We describe steps for cell synchronization, cell plating, SPY-DNA labeling, and live-cell imaging. We then detail procedures for surface plot visualization and intensity profile analysis. This approach enables direct comparison of mitotic progression between adherent and suspension cells. For complete details on the use and execution of this protocol, please refer to Kamranvar et al.¹.
SN  - 2666-1667
ER  -