Protocol for generating human organoid tumor transplantation and immune-human organoid tumor transplantation models for glioblastoma research
- 1Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
- 2Department of Neurosurgery, UCLA Health, Los Angeles, CA 90095, USA
Abstract
Glioblastoma research requires human models that preserve tumor heterogeneity and microenvironmental interactions. Here, we present a protocol for generating the human organoid tumor transplantation (HOTT) model and its immunocompetent counterpart, the immune-HOTT (iHOTT) model. These platforms use direct-from-patient tumor cells engrafted into organoids to recapitulate the tumor microenvironment and, in the iHOTT system, autologous tumor-immune interactions. We describe steps for organoid generation, patient tumor dissociation, co-culture with autologous peripheral blood mononuclear cells, fluorescence-activated cell sorting, and downstream single-cell RNA sequencing.
For complete details on the use and execution of this protocol, please refer to Ge et al. and Baisiwala et al.1,2
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BibTeX
@article{li2026protocol,
title = {{Protocol for generating human organoid tumor transplantation and immune-human organoid tumor transplantation models for glioblastoma research}},
author = {Matthew X. Li and Weihong Ge and Elisa Fazzari and Aparna Bhaduri and Shivani Baisiwala},
journal = {STAR Protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2026.104925},
url = {https://doi.org/10.1016/j.xpro.2026.104925},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Protocol for generating human organoid tumor transplantation and immune-human organoid tumor transplantation models for glioblastoma research
AU - Matthew X. Li
AU - Weihong Ge
AU - Elisa Fazzari
AU - Aparna Bhaduri
AU - Shivani Baisiwala
PY - 2026
DA - 2026/10/30
JO - STAR Protocols
DO - 10.1016/j.xpro.2026.104925
UR - https://doi.org/10.1016/j.xpro.2026.104925
AB - Glioblastoma research requires human models that preserve tumor heterogeneity and microenvironmental interactions. Here, we present a protocol for generating the human organoid tumor transplantation (HOTT) model and its immunocompetent counterpart, the immune-HOTT (iHOTT) model. These platforms use direct-from-patient tumor cells engrafted into organoids to recapitulate the tumor microenvironment and, in the iHOTT system, autologous tumor-immune interactions. We describe steps for organoid generation, patient tumor dissociation, co-culture with autologous peripheral blood mononuclear cells, fluorescence-activated cell sorting, and downstream single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. and Baisiwala et al.1,2
SN - 2666-1667
ER -