Prime-pull immunisation with yeast-based vaccine modifies cell trafficking and T-cell immunity in bovine mammary gland
Abstract
Mastitis remains a major challenge for the dairy industry and the limited efficacy of commercially available vaccines is attributed to their inability to induce strong local cell-mediated immunity. As alternatives, yeast shows great promise, acting as antigen vectors, production platforms and natural adjuvants inducing robust cellular responses. We have previously shown that strategies to counterbalance the inherent immunogenicity of yeast-based vaccines are necessary to enhance host response to vaccinal antigens. In this study, we vaccinated cows with an improved bivalent Saccharomyces cerevisiae-based formulation using a prime-pull regimen and employed multiple approaches to provide deep insight into the impact of immunisation on the establishment of immune mechanisms in the mammary gland. We provide evidence that our vaccination strategy modulates cell trafficking and promotes memory T-cell responses in the mammary gland. Our results also suggest that immunising all quarters may not be necessary to elicit immune responses at the whole mammary gland.
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BibTeX
@article{danzelle2026primepull,
title = {{Prime-pull immunisation with yeast-based vaccine modifies cell trafficking and T-cell immunity in bovine mammary gland}},
author = {Célya Danzelle and Pablo Gomes Noleto and Patricia Cunha and Florence B Gilbert and Yves Le Vern and Olivier Boulesteix and Pierre Germon and Pascal Rainard and Marc Blondel and Ignacio Caballero-Posadas and Rodrigo Prado Martins},
journal = {NPJ vaccines},
year = {2026},
month = sep,
doi = {10.1038/s41541-026-01584-9},
url = {https://hal.science/hal-05787118v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Prime-pull immunisation with yeast-based vaccine modifies cell trafficking and T-cell immunity in bovine mammary gland
AU - Célya Danzelle
AU - Pablo Gomes Noleto
AU - Patricia Cunha
AU - Florence B Gilbert
AU - Yves Le Vern
AU - Olivier Boulesteix
AU - Pierre Germon
AU - Pascal Rainard
AU - Marc Blondel
AU - Ignacio Caballero-Posadas
AU - Rodrigo Prado Martins
PY - 2026
DA - 2026/09/26
JO - NPJ vaccines
DO - 10.1038/s41541-026-01584-9
UR - https://hal.science/hal-05787118v1
AB - Mastitis remains a major challenge for the dairy industry and the limited efficacy of commercially available vaccines is attributed to their inability to induce strong local cell-mediated immunity. As alternatives, yeast shows great promise, acting as antigen vectors, production platforms and natural adjuvants inducing robust cellular responses. We have previously shown that strategies to counterbalance the inherent immunogenicity of yeast-based vaccines are necessary to enhance host response to vaccinal antigens. In this study, we vaccinated cows with an improved bivalent Saccharomyces cerevisiae-based formulation using a prime-pull regimen and employed multiple approaches to provide deep insight into the impact of immunisation on the establishment of immune mechanisms in the mammary gland. We provide evidence that our vaccination strategy modulates cell trafficking and promotes memory T-cell responses in the mammary gland. Our results also suggest that immunising all quarters may not be necessary to elicit immune responses at the whole mammary gland.
SN - 2059-0105
ER -