Effect of type-2 diabetes on surface lipids and biophysical properties in lipoprotein populations

Abstract

Surface lipids in lipoproteins interact with proteins and receptors at the surface of cell membranes, mediating lipoprotein-cell signaling and trafficking events. Despite this, little is known about lipoproteins' surface lipid composition and any changes introduced by type-2 diabetes mellitus (T2DM). Herein, we investigate the surface lipid composition in purified VLDL, LDL and HDL populations isolated from patients with poor (PC, HbA1c>8.5%) and good glycemic control (GC, HbA1c<6.5%) using high-performance thin-layer chromatography (HPTLC) and targeted liquid chromatography-mass spectrometry (LC-MRM-MS) approaches, which were complemented with biophysical measurements. Composed largely of free cholesterol (Chol), phosphatidylcholine (PC), and sphingomyelin (SM), the surface of lipoproteins contains minor quantities of phosphatidylethanolamine (PE), phosphatidylinositol (PI), monoglycerides (MG), ceramides (Cer) and glucosyl-ceramides (GlcCer). Our findings show that PE levels are altered in VLDL and LDL in T2DM. Screening of predominant Chol and PC lipids derivatives, cholesterol sulfate (CholS) and oxidized phosphatidylcholines (oxPC), revealed pronounced changes in LDL. At the biophysical level, T2DM impacted VLDL and HDL surface properties but not those of LDL. The distinct behavior of lipoproteins' composition and biophysical parameters in T2DM patients and in response to medication, diet, and physical exercise, may contribute to particle-membrane interactions.

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BibTeX RIS

BibTeX

@article{reis2026effect,
  title = {{Effect of type-2 diabetes on surface lipids and biophysical properties in lipoprotein populations}},
  author = {Ana Reis and Ana Rita D. Araújo and Irundika Hk. Dias and Bruno Antonny and Antonio Perez and Jose Luis Sánchez-Quesada and Victor de Freitas},
  journal = {Archives of Biochemistry and Biophysics},
  year = {2026},
  month = nov,
  doi = {10.1016/j.abb.2026.110780},
  url = {https://hal.science/hal-05852351v1},
  note = {CC BY 4.0},
}

RIS

TY  - JOUR
TI  - Effect of type-2 diabetes on surface lipids and biophysical properties in lipoprotein populations
AU  - Ana Reis
AU  - Ana Rita D. Araújo
AU  - Irundika Hk. Dias
AU  - Bruno Antonny
AU  - Antonio Perez
AU  - Jose Luis Sánchez-Quesada
AU  - Victor de Freitas
PY  - 2026
DA  - 2026/11/04
JO  - Archives of Biochemistry and Biophysics
DO  - 10.1016/j.abb.2026.110780
UR  - https://hal.science/hal-05852351v1
AB  - Surface lipids in lipoproteins interact with proteins and receptors at the surface of cell membranes, mediating lipoprotein-cell signaling and trafficking events. Despite this, little is known about lipoproteins' surface lipid composition and any changes introduced by type-2 diabetes mellitus (T2DM). Herein, we investigate the surface lipid composition in purified VLDL, LDL and HDL populations isolated from patients with poor (PC, HbA1c>8.5%) and good glycemic control (GC, HbA1c<6.5%) using high-performance thin-layer chromatography (HPTLC) and targeted liquid chromatography-mass spectrometry (LC-MRM-MS) approaches, which were complemented with biophysical measurements. Composed largely of free cholesterol (Chol), phosphatidylcholine (PC), and sphingomyelin (SM), the surface of lipoproteins contains minor quantities of phosphatidylethanolamine (PE), phosphatidylinositol (PI), monoglycerides (MG), ceramides (Cer) and glucosyl-ceramides (GlcCer). Our findings show that PE levels are altered in VLDL and LDL in T2DM. Screening of predominant Chol and PC lipids derivatives, cholesterol sulfate (CholS) and oxidized phosphatidylcholines (oxPC), revealed pronounced changes in LDL. At the biophysical level, T2DM impacted VLDL and HDL surface properties but not those of LDL. The distinct behavior of lipoproteins' composition and biophysical parameters in T2DM patients and in response to medication, diet, and physical exercise, may contribute to particle-membrane interactions.
SN  - 0003-9861
SN  - 1096-0384
ER  -

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