Shanyu Wan attenuates age-related muscle alterations in SAMP8 mice with improved mitochondrial function
- 1Rehabilitation Industry Institute, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China
Abstract
Background and aim: The traditional Chinese formula Shanyu Wan (SYW), comprising Panax ginseng, Dioscorea opposita Thunb and Atractylodes Rhizoma, has been empirically employed for metabolic regulation, although its mechanistic basis remains incompletely elucidated.
Experimental procedure: Senescence-accelerated mouse prone 8 (SAMP8) mice were orally administered SYW by gavage at 2.56 g/kg for 8 weeks, with age-matched senescence-accelerated mouse resistant 1 (SAMR1) mice serving as controls. Muscle phenotype was evaluated by forelimb grip strength, rotarod performance, spontaneous locomotor activity, and magnetic resonance imaging (MRI). Histopathological alterations were evaluated by Masson's trichrome staining. Mitochondrial ultrastructure was examined by transmission electron microscopy (TEM), and mitochondrial functional parameters were subsequently determined. Furthermore, the effects of SYW-containing serum on oxidative injury were examined in t-BHP-treated C2C12 myoblasts, together with pharmacological interference analysis of PGC-1α-related signaling.
Results and conclusion: Oral administration of SYW significantly improved muscle volume, grip strength, and motor performance in SAMP8 mice. These protective effects were accompanied by partial restoration of mitochondrial ultrastructure, increased mitochondrial respiratory chain complex activity, reduced oxidative stress, and changes in PGC-1α-related signaling. In vitro, SYW-containing serum attenuated t-BHP-induced oxidative and mitochondrial injury in C2C12 myoblasts, while pharmacological interference experiments suggested the involvement of PGC-1α-related signaling. These findings provide preclinical evidence that SYW may alleviate age-related muscle alterations by improving mitochondrial function and redox balance.
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BibTeX
@article{hu2026shanyu,
title = {{Shanyu Wan attenuates age-related muscle alterations in SAMP8 mice with improved mitochondrial function}},
author = {Yue Hu and Qing Xiang and Cai Jiang and Chunxiu Huang and Huanghao Zhou and Yanyan Lan and Huijuan Wu and Zuanfang Li and Xiaodan Wang and Cong Chen and Lidian Chen},
journal = {Journal of traditional and complementary medicine},
year = {2026},
month = jul,
doi = {10.1016/j.jtcme.2026.07.002},
url = {https://europepmc.org/article/PMC/PMC13729021},
note = {CC BY},
}
RIS
TY - JOUR
TI - Shanyu Wan attenuates age-related muscle alterations in SAMP8 mice with improved mitochondrial function
AU - Yue Hu
AU - Qing Xiang
AU - Cai Jiang
AU - Chunxiu Huang
AU - Huanghao Zhou
AU - Yanyan Lan
AU - Huijuan Wu
AU - Zuanfang Li
AU - Xiaodan Wang
AU - Cong Chen
AU - Lidian Chen
PY - 2026
DA - 2026/07/22
JO - Journal of traditional and complementary medicine
DO - 10.1016/j.jtcme.2026.07.002
UR - https://europepmc.org/article/PMC/PMC13729021
AB - Background and aim: The traditional Chinese formula Shanyu Wan (SYW), comprising Panax ginseng, Dioscorea opposita Thunb and Atractylodes Rhizoma, has been empirically employed for metabolic regulation, although its mechanistic basis remains incompletely elucidated. Experimental procedure: Senescence-accelerated mouse prone 8 (SAMP8) mice were orally administered SYW by gavage at 2.56 g/kg for 8 weeks, with age-matched senescence-accelerated mouse resistant 1 (SAMR1) mice serving as controls. Muscle phenotype was evaluated by forelimb grip strength, rotarod performance, spontaneous locomotor activity, and magnetic resonance imaging (MRI). Histopathological alterations were evaluated by Masson's trichrome staining. Mitochondrial ultrastructure was examined by transmission electron microscopy (TEM), and mitochondrial functional parameters were subsequently determined. Furthermore, the effects of SYW-containing serum on oxidative injury were examined in t-BHP-treated C2C12 myoblasts, together with pharmacological interference analysis of PGC-1α-related signaling. Results and conclusion: Oral administration of SYW significantly improved muscle volume, grip strength, and motor performance in SAMP8 mice. These protective effects were accompanied by partial restoration of mitochondrial ultrastructure, increased mitochondrial respiratory chain complex activity, reduced oxidative stress, and changes in PGC-1α-related signaling. In vitro, SYW-containing serum attenuated t-BHP-induced oxidative and mitochondrial injury in C2C12 myoblasts, while pharmacological interference experiments suggested the involvement of PGC-1α-related signaling. These findings provide preclinical evidence that SYW may alleviate age-related muscle alterations by improving mitochondrial function and redox balance.
SN - 2225-4110
ER -