Physicochemical quality, molecular composition, tocopherol profiling, and in vitro activity of cold-pressed Sacha inchi (Plukenetia volubilis L.) oil
Abstract
Sacha inchi ( Plukenetia volubilis L.) oil is among the richest plant-derived sources of alpha-linolenic acid (ALA), offering vegetarians a plant-based route to omega-3 fatty acid. This study encompasses around the characterization of cold-pressed Sacha inchi oil (SIO) combining physicochemical quality assessment and molecular composition. GC-FID analysis quantified five major fatty acids, with ALA as the predominant fatty acid (49.61 ± 0.05%), with total PUFA content of 84.25 ± 0.07%, with a ω-6:ω-3 ratio of 0.70:1. LC–MS/MS analysis revealed a total tocopherol content of 492.685 ± 0.049 mg kg⁻¹. SIO exhibited concentration-dependent DPPH (IC₅₀ = 24.94 ± 2.58 µg mL⁻¹) and ABTS radical scavenging activity (IC₅₀ = 31.99 ± 1.13 µg mL⁻¹). In RAW 264.7 macrophages, SIO showed concentration-dependent effects on cell viability, with IC₅₀ values of 213.40 ± 5.73 µg/mL in LPS untreated cells. SIO also reduced LPS-induced NO production by 6.05–49.62% at 10–75 µg mL⁻¹. And exhibits cytotoxicity against MDA-MB-231 triple-negative breast cancer cells (IC₅₀ = 15.08 µg mL⁻¹; 95% CI: 14.14–16.06 µg mL⁻¹) and HEK-293 cells (IC₅₀ = 45.53 µg mL⁻¹; 95% CI: 43.02–48.02 µg mL⁻¹). These findings highlight the compositional richness of SIO and support its potential as PUFA-rich functional food ingredient.
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BibTeX
@article{shanmugam2026physicochemical,
title = {{Physicochemical quality, molecular composition, tocopherol profiling, and in vitro activity of cold-pressed Sacha inchi (Plukenetia volubilis L.) oil}},
author = {Gobika Shanmugam and Renganathan Sahadevan},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-75571-4},
url = {https://www.nature.com/articles/s41598-026-75571-4},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Physicochemical quality, molecular composition, tocopherol profiling, and in vitro activity of cold-pressed Sacha inchi (Plukenetia volubilis L.) oil
AU - Gobika Shanmugam
AU - Renganathan Sahadevan
PY - 2026
DA - 2026/10/11
JO - Scientific Reports
DO - 10.1038/s41598-026-75571-4
UR - https://www.nature.com/articles/s41598-026-75571-4
AB - Sacha inchi ( Plukenetia volubilis L.) oil is among the richest plant-derived sources of alpha-linolenic acid (ALA), offering vegetarians a plant-based route to omega-3 fatty acid. This study encompasses around the characterization of cold-pressed Sacha inchi oil (SIO) combining physicochemical quality assessment and molecular composition. GC-FID analysis quantified five major fatty acids, with ALA as the predominant fatty acid (49.61 ± 0.05%), with total PUFA content of 84.25 ± 0.07%, with a ω-6:ω-3 ratio of 0.70:1. LC–MS/MS analysis revealed a total tocopherol content of 492.685 ± 0.049 mg kg⁻¹. SIO exhibited concentration-dependent DPPH (IC₅₀ = 24.94 ± 2.58 µg mL⁻¹) and ABTS radical scavenging activity (IC₅₀ = 31.99 ± 1.13 µg mL⁻¹). In RAW 264.7 macrophages, SIO showed concentration-dependent effects on cell viability, with IC₅₀ values of 213.40 ± 5.73 µg/mL in LPS untreated cells. SIO also reduced LPS-induced NO production by 6.05–49.62% at 10–75 µg mL⁻¹. And exhibits cytotoxicity against MDA-MB-231 triple-negative breast cancer cells (IC₅₀ = 15.08 µg mL⁻¹; 95% CI: 14.14–16.06 µg mL⁻¹) and HEK-293 cells (IC₅₀ = 45.53 µg mL⁻¹; 95% CI: 43.02–48.02 µg mL⁻¹). These findings highlight the compositional richness of SIO and support its potential as PUFA-rich functional food ingredient.
SN - 2045-2322
ER -