CBL Gene Family in Brassica napus: Genome-Wide and Expression Profiling in Response to Phytohormones Under Diverse Stress Conditions
- 1Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China
Abstract
Brassica napus L. is a globally important crop and its productivity is constrained by multiple abiotic stresses (salinity, drought, and heat). Calcineurin B-like proteins (CBLs) act as calcium sensors and play key roles in regulating ion homeostasis and stress-responsive signaling pathways, thereby contributing to plant adaptation under unfavorable environmental conditions. Here, through detailed bioinformatics analyses, the BnCBL gene family has been identified along with its role in tolerance to multiple abiotic stresses. The identified 17 BnCBLs comprised four groups, as in Arabidopsis thaliana. The predicted molecular weights of the CBL proteins ranged from approximately 24.35 kDa (BnCBL3 and -9) to 29.7 kDa (BnCBL5), with protein lengths spanning 213 (BnCBL3, -9, -10, -12 and -15) to 260 amino acids (BnCBL5). Sequence, promoter, and structural analyses showed that BnCBL proteins harbor palmitoylation and myristoylation motifs in their EF-hand domains, contain hormone- and stress-responsive cis-elements, and exhibit characteristic post-translational modification sites and tertiary structures. RNA-seq and RT-qPCR expression analyses showed that several BnCBL genes (BnCBL2, -6, -9, -10, and -15) exhibit differential expression (3~6-fold) under NaCl, drought, and heat stresses, as well as in response to phytohormones (IAA, GA3, ABA, and JA). In addition, BnCBL2, -3, -6, -8, -9, -11, -12 and -16 showed significant expression (around 7-fold) against biotic stresses (Sclerotinia sclerotiorum (Lib.) de Bary and Plasmodiophora brassicae (Woronin, 1877), indicating their roles in both biotic and abiotic stress tolerance and potential utility in biotechnological breeding of stress-enduring B. napus cultivars.
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BibTeX
@article{zhang2026gene,
title = {{CBL Gene Family in Brassica napus: Genome-Wide and Expression Profiling in Response to Phytohormones Under Diverse Stress Conditions}},
author = {Renyi Zhang and Kexin Liang and Zimo Qiu and Dexi Shi and Shuang He and Guangqi Zhu and Beibei Xu and Iqbal Hussain and Jiabao Huang and Rana Muhammad Amir Gulzar},
journal = {Agriculture},
year = {2026},
month = may,
doi = {10.3390/agriculture16101088},
url = {https://doi.org/10.3390/agriculture16101088},
note = {CC BY},
}
RIS
TY - JOUR
TI - CBL Gene Family in Brassica napus: Genome-Wide and Expression Profiling in Response to Phytohormones Under Diverse Stress Conditions
AU - Renyi Zhang
AU - Kexin Liang
AU - Zimo Qiu
AU - Dexi Shi
AU - Shuang He
AU - Guangqi Zhu
AU - Beibei Xu
AU - Iqbal Hussain
AU - Jiabao Huang
AU - Rana Muhammad Amir Gulzar
PY - 2026
DA - 2026/05/15
JO - Agriculture
DO - 10.3390/agriculture16101088
UR - https://doi.org/10.3390/agriculture16101088
AB - Brassica napus L. is a globally important crop and its productivity is constrained by multiple abiotic stresses (salinity, drought, and heat). Calcineurin B-like proteins (CBLs) act as calcium sensors and play key roles in regulating ion homeostasis and stress-responsive signaling pathways, thereby contributing to plant adaptation under unfavorable environmental conditions. Here, through detailed bioinformatics analyses, the BnCBL gene family has been identified along with its role in tolerance to multiple abiotic stresses. The identified 17 BnCBLs comprised four groups, as in Arabidopsis thaliana. The predicted molecular weights of the CBL proteins ranged from approximately 24.35 kDa (BnCBL3 and -9) to 29.7 kDa (BnCBL5), with protein lengths spanning 213 (BnCBL3, -9, -10, -12 and -15) to 260 amino acids (BnCBL5). Sequence, promoter, and structural analyses showed that BnCBL proteins harbor palmitoylation and myristoylation motifs in their EF-hand domains, contain hormone- and stress-responsive cis-elements, and exhibit characteristic post-translational modification sites and tertiary structures. RNA-seq and RT-qPCR expression analyses showed that several BnCBL genes (BnCBL2, -6, -9, -10, and -15) exhibit differential expression (3~6-fold) under NaCl, drought, and heat stresses, as well as in response to phytohormones (IAA, GA3, ABA, and JA). In addition, BnCBL2, -3, -6, -8, -9, -11, -12 and -16 showed significant expression (around 7-fold) against biotic stresses (Sclerotinia sclerotiorum (Lib.) de Bary and Plasmodiophora brassicae (Woronin, 1877), indicating their roles in both biotic and abiotic stress tolerance and potential utility in biotechnological breeding of stress-enduring B. napus cultivars.
SN - 2077-0472
ER -