Multiscale Decoupling in Peptide-Membrane Interactions: From Elastic Softening to Domain-Mediated Stiffening
Abstract
Membrane composition plays a central role in determining how antimicrobial peptides (AMPs) interact with and alter the physical properties of lipid membranes. Here, we investigate the composition-dependent mechanical and structural response of lipid membranes to the AMP aurein using neutron spin-echo (NSE) spectroscopy, neutron membrane diffraction (NMD), and pressure (P)-area (A) isotherms, complemented by previously reported quasielastic neutron scattering (QENS) measurements. In a zwitterionic DMPC membrane, aurein induces progressive softening, manifested by a concentration-dependent decrease in both the bending rigidity and area compressibility modulus. NMD reveals a slight bilayer thinning but no prominent peptide-associated contribution within the deuterated hydrocarbon region, indicating that the progressive mechanical softening is not accompanied by increasingly deep peptide penetration into the hydrophobic core. In contrast, anionic phosphatidylglycerol (PG)-containing DMPC/DMPG membranes exhibit a non-monotonic response, with initial softening followed by pronounced stiffening at higher peptide concentrations. Complementary P-A isotherm measurements independently reproduce these contrasting mechanical responses, showing progressive softening of DMPC but stiffening of DMPC/DMPG. Most importantly, the combined NSE and QENS results reveal a striking multiscale decoupling: aurein suppresses molecular-scale lipid lateral diffusion while simultaneously reducing collective membrane stiffness at the mesoscopic scale, demonstrating that molecular lipid dynamics and collective membrane mechanics can evolve in opposite directions under the same peptide perturbation.Together, these results show that membrane composition governs the collective mechanical response to aurein, while molecular lipid dynamics can respond independently at shorter length and time scales.
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BibTeX
@misc{sharma2026multiscale,
title = {{Multiscale Decoupling in Peptide-Membrane Interactions: From Elastic Softening to Domain-Mediated Stiffening}},
author = {Veerendra K. Sharma and Shuo Qian and Gergely Nagy and Sonam Raghav and Sajal K. Ghosh},
howpublished = {arXiv},
year = {2026},
month = oct,
doi = {10.48550/arxiv.2610.09595},
eprint = {2610.09595},
archivePrefix = {arXiv},
url = {https://arxiv.org/abs/2610.09595},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - UNPB
TI - Multiscale Decoupling in Peptide-Membrane Interactions: From Elastic Softening to Domain-Mediated Stiffening
AU - Veerendra K. Sharma
AU - Shuo Qian
AU - Gergely Nagy
AU - Sonam Raghav
AU - Sajal K. Ghosh
PY - 2026
DA - 2026/10/07
PB - arXiv
DO - 10.48550/arxiv.2610.09595
UR - https://arxiv.org/abs/2610.09595
AB - Membrane composition plays a central role in determining how antimicrobial peptides (AMPs) interact with and alter the physical properties of lipid membranes. Here, we investigate the composition-dependent mechanical and structural response of lipid membranes to the AMP aurein using neutron spin-echo (NSE) spectroscopy, neutron membrane diffraction (NMD), and pressure (P)-area (A) isotherms, complemented by previously reported quasielastic neutron scattering (QENS) measurements. In a zwitterionic DMPC membrane, aurein induces progressive softening, manifested by a concentration-dependent decrease in both the bending rigidity and area compressibility modulus. NMD reveals a slight bilayer thinning but no prominent peptide-associated contribution within the deuterated hydrocarbon region, indicating that the progressive mechanical softening is not accompanied by increasingly deep peptide penetration into the hydrophobic core. In contrast, anionic phosphatidylglycerol (PG)-containing DMPC/DMPG membranes exhibit a non-monotonic response, with initial softening followed by pronounced stiffening at higher peptide concentrations. Complementary P-A isotherm measurements independently reproduce these contrasting mechanical responses, showing progressive softening of DMPC but stiffening of DMPC/DMPG. Most importantly, the combined NSE and QENS results reveal a striking multiscale decoupling: aurein suppresses molecular-scale lipid lateral diffusion while simultaneously reducing collective membrane stiffness at the mesoscopic scale, demonstrating that molecular lipid dynamics and collective membrane mechanics can evolve in opposite directions under the same peptide perturbation.Together, these results show that membrane composition governs the collective mechanical response to aurein, while molecular lipid dynamics can respond independently at shorter length and time scales.
ER -