Effects of Rosmarinic Acid on L-Methionine-Induced Behavioral Impairments and Disruptions in Hippocampal Neurogenesis in the Adult Rat.
5 affiliations
- 1Department of Basic Medical Science, Faculty of Medicine Vajira Hospital Navamindradhiraj University Bangkok Thailand.
- 2Neurogenesis Research Group, Department of Anatomy, Faculty of Medicine Khon Kaen University Khon Kaen Thailand.
- 3Department of Anatomy, Faculty of Medicine Khon Kaen University Khon Kaen Thailand.
- 4Faculty of Medicine Mahasarakham University Mahasarakham Thailand.
- 5School of Life Sciences, Medical School, Queen's Medical Centre University of Nottingham Nottingham UK.
Abstract
L-methionine (L-met) is an essential amino acid; however, excessive intake induces oxidative stress associated with memory impairment and reduced hippocampal neurogenesis. Rosmarinic acid (RA), a natural phenolic compound, has been reported to exhibit antioxidant and neuroprotective properties. This study aimed to investigate the effects of RA on L-met-induced memory deficits and alterations in hippocampal neurogenesis in adult rats. Fifty-four male Sprague-Dawley rats were divided into 6 groups: control, L-met (1.7 g/kg/day), RA10 (10 mg/kg/day), RA30 (30 mg/kg/day), L-met + RA10 (L-met: 1.7 g/kg/day and RA: 10 mg/kg/day), and L-met + RA30 (L-met: 1.7 g/kg/day and RA: 30 mg/kg/day). Memory performance was evaluated using the novel object location (NOL) and novel object recognition (NOR) tests. Hippocampal neurogenesis was assessed using Ki-67 and Ki-67/rat endothelial cell antigen-1 (RECA-1) as markers of cell proliferation and vascular-associated proliferating cells, 5-bromo-2'-deoxyuridine/neuronal nuclear protein (BrdU/NeuN) as a marker of neuronal survival associated with mature neurons and p21 as a marker of cell cycle arrest. The results revealed that L-met administration significantly impaired memory performance, reduced Ki-67-, Ki-67/RECA-1-, and BrdU/NeuN-positive cells, and increased p21-positive cells. However, co-administration of RA (10 and 30 mg/kg) significantly attenuated L-met-induced memory deficits and hippocampal neurogenesis-related impairments. These findings suggest that RA attenuates L-met-induced memory impairment and disruption of hippocampal neurogenesis in adult rats.
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BibTeX
@article{naewla2026effects,
title = {{Effects of Rosmarinic Acid on L-Methionine-Induced Behavioral Impairments and Disruptions in Hippocampal Neurogenesis in the Adult Rat.}},
author = {Salinee Naewla and Jirawadee Chaithum and Tanaporn Anosri and Nataya Sritawan and Anusara Aranarochana and Jariya Umka Welbat and Peter Wigmore and Apiwat Sirichoat},
journal = {Food science \& nutrition},
year = {2026},
month = oct,
doi = {10.1002/fsn3.72502},
url = {https://europepmc.org/article/MED/42948199},
note = {CC BY},
}
RIS
TY - JOUR
TI - Effects of Rosmarinic Acid on L-Methionine-Induced Behavioral Impairments and Disruptions in Hippocampal Neurogenesis in the Adult Rat.
AU - Salinee Naewla
AU - Jirawadee Chaithum
AU - Tanaporn Anosri
AU - Nataya Sritawan
AU - Anusara Aranarochana
AU - Jariya Umka Welbat
AU - Peter Wigmore
AU - Apiwat Sirichoat
PY - 2026
DA - 2026/10/26
JO - Food science & nutrition
DO - 10.1002/fsn3.72502
UR - https://europepmc.org/article/MED/42948199
AB - L-methionine (L-met) is an essential amino acid; however, excessive intake induces oxidative stress associated with memory impairment and reduced hippocampal neurogenesis. Rosmarinic acid (RA), a natural phenolic compound, has been reported to exhibit antioxidant and neuroprotective properties. This study aimed to investigate the effects of RA on L-met-induced memory deficits and alterations in hippocampal neurogenesis in adult rats. Fifty-four male Sprague-Dawley rats were divided into 6 groups: control, L-met (1.7 g/kg/day), RA10 (10 mg/kg/day), RA30 (30 mg/kg/day), L-met + RA10 (L-met: 1.7 g/kg/day and RA: 10 mg/kg/day), and L-met + RA30 (L-met: 1.7 g/kg/day and RA: 30 mg/kg/day). Memory performance was evaluated using the novel object location (NOL) and novel object recognition (NOR) tests. Hippocampal neurogenesis was assessed using Ki-67 and Ki-67/rat endothelial cell antigen-1 (RECA-1) as markers of cell proliferation and vascular-associated proliferating cells, 5-bromo-2'-deoxyuridine/neuronal nuclear protein (BrdU/NeuN) as a marker of neuronal survival associated with mature neurons and p21 as a marker of cell cycle arrest. The results revealed that L-met administration significantly impaired memory performance, reduced Ki-67-, Ki-67/RECA-1-, and BrdU/NeuN-positive cells, and increased p21-positive cells. However, co-administration of RA (10 and 30 mg/kg) significantly attenuated L-met-induced memory deficits and hippocampal neurogenesis-related impairments. These findings suggest that RA attenuates L-met-induced memory impairment and disruption of hippocampal neurogenesis in adult rats.
SN - 2048-7177
ER -