The cell polarity landscape in Arabidopsis male and female gametophytes
- 1Wageningen University
Abstract
Plant cell polarity underlies the capacity to directionally transport auxin and other small molecules, but also precedes asymmetric cell division in the stomatal lineage. Except for specific contexts, mechanisms underlying cell polarization are poorly understood. Most insights on plant cell polarity have come from studies on flowering plant sporophytes, while no polar protein localization has yet been reported in flowering plant gametophytes, despite clear anisotropic growth and asymmetric cell divisions in this generation. Inspired by the identification of deeply conserved SOSEKI proteins and their associated partners that mark polar domains in bryophyte gametophytes and Arabidopsis sporophytes, we here investigate cell polarity in Arabidopsis gametophytes. We discover a rich landscape of polar domains throughout male and female gametophyte development, marked by one or more polar proteins. We find that successive steps in male gametophyte polarization can be mechanistically uncoupled, and the first polarization step precedes asymmetric cell division. Using ectopic expression, we find that there is a near-universal capacity for polar protein localization in gametophytes through a single polarity system. Our work offers the first insight into gametophyte cell polarity in Arabidopsis, identifies novel polar domains, and presents a starting point for future dissection of mechanisms underlying plant cell polarity.
Related
- An embryo-derived peptide signal directs endosperm polarity in Arabidopsis
- Polymorphism can extensively reshape the genome-wide crossover landscape in Arabidopsis thaliana
- Pulling the Back to Steer Cell Migration: Mechanisms of Structural Polarity in Confining Signaling and Protrusion
- Planar cell polarity aligns epithelial migration to coordinate intestinal architecture and functional zonation
- A cyclin-polarity feedback network ensures healthy cell proliferation
BibTeX
@misc{han2026cell,
title = {{The cell polarity landscape in Arabidopsis male and female gametophytes}},
author = {S. Koh Wee Han and M. van Schie and C. Albrecht and J. Ramalho and B. Voss and D. Weijers},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.30.763139},
url = {https://www.biorxiv.org/content/10.64898/2026.10.30.763139v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - The cell polarity landscape in Arabidopsis male and female gametophytes
AU - S. Koh Wee Han
AU - M. van Schie
AU - C. Albrecht
AU - J. Ramalho
AU - B. Voss
AU - D. Weijers
PY - 2026
DA - 2026/10/30
PB - bioRxiv
DO - 10.64898/2026.10.30.763139
UR - https://www.biorxiv.org/content/10.64898/2026.10.30.763139v1
AB - Plant cell polarity underlies the capacity to directionally transport auxin and other small molecules, but also precedes asymmetric cell division in the stomatal lineage. Except for specific contexts, mechanisms underlying cell polarization are poorly understood. Most insights on plant cell polarity have come from studies on flowering plant sporophytes, while no polar protein localization has yet been reported in flowering plant gametophytes, despite clear anisotropic growth and asymmetric cell divisions in this generation. Inspired by the identification of deeply conserved SOSEKI proteins and their associated partners that mark polar domains in bryophyte gametophytes and Arabidopsis sporophytes, we here investigate cell polarity in Arabidopsis gametophytes. We discover a rich landscape of polar domains throughout male and female gametophyte development, marked by one or more polar proteins. We find that successive steps in male gametophyte polarization can be mechanistically uncoupled, and the first polarization step precedes asymmetric cell division. Using ectopic expression, we find that there is a near-universal capacity for polar protein localization in gametophytes through a single polarity system. Our work offers the first insight into gametophyte cell polarity in Arabidopsis, identifies novel polar domains, and presents a starting point for future dissection of mechanisms underlying plant cell polarity.
ER -