Purified and HPCCC Fractionated Anthocyanin-Rich Extracts from Black Bean Hulls Modulate H2O2-Induced Intracellular ROS Generation in RAW 264.7 Cells
4 affiliations
- 1School of Engineering and Sciences, Tecnológico de Monterrey, Monterrey 64700, Nuevo León, Mexico
- 2Food Technology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Camino Arenero 1227, Col. El Bajío, Zapopan 45019, Jalisco, Mexico
- 3Biotechnology Engineering, Instituto Tecnológico y de Estudios Superiores de Occidente, Anillo Periférico Sur Manuel Gómez Morín 8585, Col. Santa María Tequepexpan, Tlaquepaque 45604, Jalisco, Mexico
- 4Industrial Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Camino Arenero 1227, Col. El Bajío, Zapopan 45019, Jalisco, Mexico
Abstract
This study aimed to obtain crude, purified, and fractionated anthocyanin-rich extracts of three black bean cultivars from the South Pacific Region of Mexico (Chiapas, Guerrero, and Oaxaca). The extracts were obtained using an Amberlite XAD-7HP column and high-performance countercurrent chromatography (HPCCC), and their modulatory effect on oxidative stress was evaluated in RAW 264.7 macrophages. Purification significantly increased the concentrations of phenolic compounds and anthocyanins across all cultivars. However, HPCCC fractionation produced a cultivar-dependent response, with marked anthocyanin enrichment observed only in the Guerrero cultivar. In contrast, a decrease was noted for Oaxaca and Chiapas, compared with the corresponding purified extracts. The anthocyanin content per gram of extract ranged from 12.1 to 21.4 mg/g for crude extracts, 27.4 to 85.5 mg/g for purified extracts, and 48 to 98.8 mg/g for fractionated extracts. The purification and fractionation processes enabled the obtaining of anthocyanin-rich extracts from black bean hulls that reduced H2O2-induced intracellular ROS generation in a cultivar- and treatment-dependent manner, with the black bean cultivar from Chiapas being the most effective, indicating that differences in its phenolic composition may contribute to the observed biological response. These findings provide valuable insights into the potential of high-performance countercurrent chromatography for the fractionation of black bean anthocyanins.
Related
- Potential anti-osteoporotic and anti-inflammatory potential of plant derived extracts and column semi-purified fractions of Epimedium brevicornum.
- Microfluidic platforms for CAR-T cell engineering: a multidisciplinary review on potential next generation intracellular delivery method
- Clinical Outcomes and Safety of Spatially Fractionated Radiotherapy for Malignant Tumors: A Systematic Review and Evidence Map
- Spatially fractionated (GRID) radiotherapy in a patient with metastatic pleomorphic rhabdomyosarcoma: a case report
- Pilot study on microwave preclinical system for targeted delivery of fractionated hyperthermia in murine solid tumors
BibTeX
@article{escobedo2026purified,
title = {{Purified and HPCCC Fractionated Anthocyanin-Rich Extracts from Black Bean Hulls Modulate H2O2-Induced Intracellular ROS Generation in RAW 264.7 Cells}},
author = {Alejandro Escobedo and Sofía Barcena and Luis Mojica and Anne Gschaedler and Eugenia Lugo-Cervantes and Montserrat Alcazar},
journal = {Molecules (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/molecules31193571},
url = {https://europepmc.org/article/PMC/PMC13667101},
note = {CC BY},
}
RIS
TY - JOUR
TI - Purified and HPCCC Fractionated Anthocyanin-Rich Extracts from Black Bean Hulls Modulate H2O2-Induced Intracellular ROS Generation in RAW 264.7 Cells
AU - Alejandro Escobedo
AU - Sofía Barcena
AU - Luis Mojica
AU - Anne Gschaedler
AU - Eugenia Lugo-Cervantes
AU - Montserrat Alcazar
PY - 2026
DA - 2026/10/01
JO - Molecules (Basel, Switzerland)
DO - 10.3390/molecules31193571
UR - https://europepmc.org/article/PMC/PMC13667101
AB - This study aimed to obtain crude, purified, and fractionated anthocyanin-rich extracts of three black bean cultivars from the South Pacific Region of Mexico (Chiapas, Guerrero, and Oaxaca). The extracts were obtained using an Amberlite XAD-7HP column and high-performance countercurrent chromatography (HPCCC), and their modulatory effect on oxidative stress was evaluated in RAW 264.7 macrophages. Purification significantly increased the concentrations of phenolic compounds and anthocyanins across all cultivars. However, HPCCC fractionation produced a cultivar-dependent response, with marked anthocyanin enrichment observed only in the Guerrero cultivar. In contrast, a decrease was noted for Oaxaca and Chiapas, compared with the corresponding purified extracts. The anthocyanin content per gram of extract ranged from 12.1 to 21.4 mg/g for crude extracts, 27.4 to 85.5 mg/g for purified extracts, and 48 to 98.8 mg/g for fractionated extracts. The purification and fractionation processes enabled the obtaining of anthocyanin-rich extracts from black bean hulls that reduced H2O2-induced intracellular ROS generation in a cultivar- and treatment-dependent manner, with the black bean cultivar from Chiapas being the most effective, indicating that differences in its phenolic composition may contribute to the observed biological response. These findings provide valuable insights into the potential of high-performance countercurrent chromatography for the fractionation of black bean anthocyanins.
SN - 1420-3049
ER -