Skip to content
DraconiticResearch
Search Sources API
190,860 studies31 sourcesUpdated 2 min ago
PreprintCC BYbioRxiv

A transcriptome map reveals conserved and lineage-specific sRNAs that regulate motility and metabolism across Vibrio

  • Z. Jia
  • H. Zhang
  • D. Falush
  • Y. Chao
  • S. L. Svensson1
  1. 1Shanghai Institute of Materia Medica / Chinese Academy of Sciences, University of Wuerzburg
Read full textPDFDOICite

Abstract

Bacterial sRNAs are pervasive post-transcriptional regulators, yet how they arise, evolve, and decay remains poorly understood. Here, we provide a high-resolution transcriptome map and curated sRNA set for the pathogen Vibrio parahaemolyticus. We identify over 100 sRNAs, including broadly conserved, lineage-specific, and previously unidentified transcripts, as well as dual-function regulatory/coding sRNAs. Functional analysis of several examples highlights conserved and lineage-specific regulators of metabolism and flagella. Broadly conserved VcrX represses chitin utilization genes and may regulate Vibrio Spot 42, which we confirm is translated. We expand on FlaX regulation of polar flagella across the genus by demonstrating that the sRNA differentially activates/represses downstream flagellins, with a potential FlaX sponge mediating feedback in specific clades. We further show that V. parahaemolyticus, but not V. cholerae, RyhB is translated into a Cys-rich small protein that could regulate related pathways. Together, these findings establish a resource for Vibrio and a platform for comparative studies of post-transcriptional regulation, enabling investigation of how sRNAs and their regulatory networks evolve.

Related

  1. Cross-regulatory sRNA networks shape adaptive stress responses in Staphylococcus aureus: RsaC and RsaOI regulate galactose metabolism and cell wall homeostasis
    H. ROBIN, M. GERMAIN, C. SILARD +4
    bioRxivPreprintAll rights reservedFull textMicrobiologybioRxiv
  2. Genetic Variations in the Folate Regulatory Enzyme ALDH1L1: Impact on Cell Metabolism, Proliferation, and Motility
    Halle M. Meyers, Amira A. Abdellatef, Natalia I. Krupenko +5
    CellsPeer-reviewedCC BY 4.0Cell biologyCrossref
  3. Forward genetics reveals a conserved CNBHD-CNBHD interface that restrains EAG channel activation to regulate sleep
    Xinyu Huang, Sudharsan Kannan, Gail A. Robertson +1
    PLOS GeneticsPeer-reviewedCC BY 4.0Full textBiophysicsNeurosciencePLOS
  4. Transcriptomic profiling reveals estradiol and progesterone specific microRNA signatures differentially regulate mRNA targets in vaginal epithelial cells
    S. Joshi, A. Nazli, C. Verschoor +1
    bioRxivPreprintAll rights reservedFull textImmunologybioRxiv
  5. Sex-specific bone marrow populations regulate skeletal phenotype and identify CD74-marked osteoclast precursor subsets
    J. Koroth, I. Y. Karkache, E. K. Vu +8
    bioRxivPreprintAll rights reservedFull textCell biologybioRxiv

Cite

BibTeXRIS
BibTeX
@misc{jia2026transcriptome,
  title = {{A transcriptome map reveals conserved and lineage-specific sRNAs that regulate motility and metabolism across Vibrio}},
  author = {Z. Jia and H. Zhang and D. Falush and Y. Chao and S. L. Svensson},
  howpublished = {bioRxiv},
  year = {2026},
  month = aug,
  doi = {10.64898/2026.08.28.747715},
  url = {https://www.biorxiv.org/content/10.64898/2026.08.28.747715v2},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - A transcriptome map reveals conserved and lineage-specific sRNAs that regulate motility and metabolism across Vibrio
AU  - Z. Jia
AU  - H. Zhang
AU  - D. Falush
AU  - Y. Chao
AU  - S. L. Svensson
PY  - 2026
DA  - 2026/08/28
PB  - bioRxiv
DO  - 10.64898/2026.08.28.747715
UR  - https://www.biorxiv.org/content/10.64898/2026.08.28.747715v2
AB  - Bacterial sRNAs are pervasive post-transcriptional regulators, yet how they arise, evolve, and decay remains poorly understood. Here, we provide a high-resolution transcriptome map and curated sRNA set for the pathogen Vibrio parahaemolyticus. We identify over 100 sRNAs, including broadly conserved, lineage-specific, and previously unidentified transcripts, as well as dual-function regulatory/coding sRNAs. Functional analysis of several examples highlights conserved and lineage-specific regulators of metabolism and flagella. Broadly conserved VcrX represses chitin utilization genes and may regulate Vibrio Spot 42, which we confirm is translated. We expand on FlaX regulation of polar flagella across the genus by demonstrating that the sRNA differentially activates/represses downstream flagellins, with a potential FlaX sponge mediating feedback in specific clades. We further show that V. parahaemolyticus, but not V. cholerae, RyhB is translated into a Cys-rich small protein that could regulate related pathways. Together, these findings establish a resource for Vibrio and a platform for comparative studies of post-transcriptional regulation, enabling investigation of how sRNAs and their regulatory networks evolve.
ER  -