Ribosome profiling reveals translational dynamics between an archaeal virus and its host
- 1Johns Hopkins University
Abstract
Host-virus interactions remain poorly understood in archaea, whose information processing systems harbor a mosaic of bacterial and eukaryotic features. Here, we used complementary multiomics to investigate interactions between Haloferax tailed virus 1 (HFTV1) and the halophilic archaeon Haloferax gibbonsii (Hg), capturing global translational and transcriptional dynamics across the infection cycle. This revealed co-regulatory expression patterns across HFTV1 and Hg genes. Post-transcriptional regulation was observed for several early HFTV1 genes and the late HFTV1 gene gp17, and the HFTV1 tRNA co-sedimented with Hg ribosome complexes. Infection reshaped the translational landscape of Hg, causing a slow decline in information processing activity, a complex transcriptional response, and amino acid starvation. An unannotated Tat-dependent fimbrial system of Hg was co-regulated with early HFTV1 genes and was correlated with increased biofilm formation, suggesting a potential antiviral defense system. These findings provide high-resolution insight into archaeal host-virus interactions at the evolutionary interface between bacteria and eukaryotes.
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BibTeX
@misc{isada2026ribosome,
title = {{Ribosome profiling reveals translational dynamics between an archaeal virus and its host}},
author = {M. F. Isada and P. Gregorova and P. Sadana and P. Sarin and J. DiRuggiero},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.13.751334},
url = {https://www.biorxiv.org/content/10.64898/2026.09.13.751334v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Ribosome profiling reveals translational dynamics between an archaeal virus and its host
AU - M. F. Isada
AU - P. Gregorova
AU - P. Sadana
AU - P. Sarin
AU - J. DiRuggiero
PY - 2026
DA - 2026/09/13
PB - bioRxiv
DO - 10.64898/2026.09.13.751334
UR - https://www.biorxiv.org/content/10.64898/2026.09.13.751334v1
AB - Host-virus interactions remain poorly understood in archaea, whose information processing systems harbor a mosaic of bacterial and eukaryotic features. Here, we used complementary multiomics to investigate interactions between Haloferax tailed virus 1 (HFTV1) and the halophilic archaeon Haloferax gibbonsii (Hg), capturing global translational and transcriptional dynamics across the infection cycle. This revealed co-regulatory expression patterns across HFTV1 and Hg genes. Post-transcriptional regulation was observed for several early HFTV1 genes and the late HFTV1 gene gp17, and the HFTV1 tRNA co-sedimented with Hg ribosome complexes. Infection reshaped the translational landscape of Hg, causing a slow decline in information processing activity, a complex transcriptional response, and amino acid starvation. An unannotated Tat-dependent fimbrial system of Hg was co-regulated with early HFTV1 genes and was correlated with increased biofilm formation, suggesting a potential antiviral defense system. These findings provide high-resolution insight into archaeal host-virus interactions at the evolutionary interface between bacteria and eukaryotes.
ER -